ST8SIA6-AS1 promotes the development of hepatocellular carcinoma cells through miR-338-3p/NONO Axis

Kuai, Jing-Hua; Li, Zheng; Yi, Xin; Liu, Zengli; Qiu, Bo; Lu, Zhihua; Jiang, Yuanhui

doi:10.1016/j.dld.2021.02.012

Cited by 9 publications

(7 citation statements)

References 17 publications

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“…Consistent with these data, Qin et al ( 51 ) revealed that the upregulation of ST8SIA6-AS1 is associated with higher tumor stages and metastasis, and thus has potential as a diagnostic biomarker for liver cancer progression. Although the role of ST8SIA6-AS1 in HBV-liver cancer cells has not been reported previously, to the best of our knowledge, the promotive effects of ST8SIA6-AS1 on cell proliferation, migration and invasion have already been elucidated in liver cancer cells ( 52 , 53 ). Consistent with the present data showing that LINC01093 was downregulated in HBV-expressing liver cancer cells and suppressed proliferation and mobility, Mou et al ( 54 ) identified LINC01093 as an optimal diagnostic and prognostic lncRNA biomarker for HBV-associated liver cancer.…”

Section: Discussionmentioning

confidence: 87%

Integrated analysis of multiple transcriptomic data identifies ST8SIA6‑AS1 and LINC01093 as potential biomarkers in HBV‑associated liver cancer

Xue¹,

Zhao²,

Fu³

et al. 2023

Oncol Lett

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The mechanisms of long-non-coding RNAs (lncRNAs) in hepatitis B virus (HBV) infection-associated liver cancer remain largely unclear. Therefore, the aim of the present study was to investigate the regulatory mechanisms of lncRNAs in this disease. HBV-liver cancer related transcriptome expression profile data (GSE121248 and GSE55092) from the Gene Expression Omnibus database and survival prognosis information from The Cancer Genome Atlas (TCGA) database were obtained for analysis. The limma package was used to identify the overlapped differentially expressed RNAs (DERs), including DElncRNAs and DEmRNAs, in the GSE121248 and GSE55092 datasets. The screened optimized lncRNA signatures were used to develop a nomogram model based on the GSE121248 dataset, which was validated using the GSE55092 and TCGA datasets. A competitive endogenous RNA (ceRNA) network was constructed based on the screened prognosis-associated lncRNA signatures from TCGA dataset. In addition, the levels of specific lncRNAs were evaluated in HBV-infected human liver cancer tissues and cells, and Cell Counting Kit-8, ELISA and Transwell assays were performed to evaluate the effects of the lncRNAs in HBV-expressing liver cancer cells. A total of 535 overlapped DERs, including 30 DElncRNAs and 505 DEmRNAs, were identified in the GSE121248 and GSE55092 datasets. An optimized DElncRNA signature comprising 10 lncRNAs was used to establish a nomogram. ST8SIA6-AS1 and LINC01093 were identified as lncRNAs associated with HBV-liver cancer prognosis in TCGA dataset, and were applied to construct a ceRNA network. Reverse transcription-quantitative PCR analysis showed that ST8SIA6-AS1 was upregulated and LINC01093 was downregulated in HBV-infected human liver cancer tissues and HBV-expressing liver cancer cells compared with non-HBV-infected controls. ST8SIA6-AS1 knockdown and LINC01093 overexpression independently reduced the number of copies of HBV DNA, the levels of hepatitis B surface antigen and hepatitis B e antigen, as well as cell proliferation, migration and invasion. In summary, the present study identified ST8SIA6-AS1 and LINC01093 as two potential biomarkers that may be effective therapeutic targets for HBV-associated liver cancer.

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Section: Discussionmentioning

confidence: 87%

Integrated analysis of multiple transcriptomic data identifies ST8SIA6‑AS1 and LINC01093 as potential biomarkers in HBV‑associated liver cancer

Xue¹,

Zhao²,

Fu³

et al. 2023

Oncol Lett

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“…Furthermore, silencing ST8SIA6-AS1 inhibits HCC cell proliferative, migratory and invasive potential [17]. Therefore, ST8SIA6-AS1 facilitates aggressive tumor phenotypes during HCC progression [11–13]. In line with the previous investigation, we also examined the highly expressed T8SIA6-AS1 in HCC tissues.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Notably, ST8SIA6-AS1 was demonstrated to display cancer-promoting properties in HCC by its ceRNA activity for targeted miRNAs and inhibiting cellular malignant behaviors [11,13]. For example, ST8SIA6-AS1 targeting miR-5195-3p hastens HCC cell proliferation, migration and resistance to apoptosis [10].…”

Section: Discussionmentioning

confidence: 99%

“…LncRNA ST8SIA6-AS1 is reported as a critical player in various cell progresses and related with different cancers, where, it confers significant oncogenic potential [8]. In HCC, ST8SIA6-AS1 was associated with advanced clinicopathological characteristics of HCC patients and regarded as a superior diagnostic biomarker [9] and plays the oncogenic role during HCC malignancy [10][11][12][13]. However, the mechanism of ST8SIA6-AS1 in HCC has not been fully understood.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA ST8SIA6-AS1 facilitates hepatocellular carcinoma progression by governing miR-651-5p/TM4SF4 axis

Mou

Ding

2022

Anti-Cancer Drugs

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The oncogenic role of ST8SIA6-AS1 in different cancers was reported, including hepatocellular carcinoma (HCC). However, the underlying mechanism has not been completely explored. Real time quantitative PCR analysis was conducted to assess the ST8SIA6-AS1, miR-651-5p and TM4SF4 expression in HCC tissues and cells. Cell counting kit-8 and wound-healing migration assays were adopted to evaluate the HCC cell proliferation and migration, respectively. The expression of apoptosis-related proteins (Bax and Bcl-2) in human colorectal cancer cells (HCC) was determined by western blotting. In addition to bioinformatics analysis, RNA immunoprecipitation studies and luciferase reporter assays were undertaken to investigate the direct target relationship among ST8SIA6-AS1 and miR-651-5p or TM4SF4. Highly expressed ST8SIA6-AS1 and TM4SF4 as well as poorly expressed miR-651-5p were detected in HCC tissues and cells. Clinically, miR-651-5p expression in HCC tissues is negatively correlated with ST8SIA6-AS1 or TM4SF4. Cell functional assays demonstrated that ST8SIA6-AS1 silencing resulted in weakened proliferative and migratory capacities in HCC cells in addition to increase Bax expression and reduced Bcl-2 expression. ST8SIA6-AS1 exhibited its oncogenic function by sponging tumor suppressor miR-651-5p, and the anti-oncogenic of miR-651-5p was offset by its TM4SF4. The manipulation of ST8SIA6-AS1/miR-651-5p/TM4SF4 axis-mediated oncogenicity in HCC might shed new light on HCC diagnosis and therapy.

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Functional roles of folic acid in alleviating dexamethasone‐induced fatty liver syndrome in laying hens

Sun,

Ma,

Wang

et al. 2024

Animal Research and One Health

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Fatty liver syndrome (FLS) poses a threat to the poultry industry due to its high occurrence and mortality rate. Folic acid (FA) is a coenzyme crucial for one‐carbon metabolism. However, the mechanism by which FA mitigates FLS in laying hens remains elusive. In this study, 60 21‐week‐old Hy‐Line Brown layers were divided into three groups: the Control (Con) group, the dexamethasone (DXM) group, and the DXM + FA group. Results showed that liver index was significantly increased in the DXM group. H&E and oil red O staining showed the accumulation of lipid droplets in the liver was intensified, confirming the successful establishment of an early fatty liver model without inflammation. FA significantly reversed hepatic lipid deposition, and 57 differentially expressed genes affected by FA were identified in the transcriptome analysis. Their transcriptional and translational levels indicate that in the early FLS, insulin‐like growth factor 2/phosphatidylinositol‐3‐kinase/protein kinase B pathway related to lipid metabolism was activated; folate cycling was inhibited, while endoplasmic reticulum (ER) stress and apoptosis‐related protein abundance were elevated. Dietary FA enhanced the folate circulation, reduced lipogenesis and ER stress, and apoptosis‐related protein expression, thereby mitigating the lipid metabolism disturbance in FLS. Metabolomics identified 151 differential metabolites involved in early FLS occurrence, 34 of which were reversed by FA. Metabolites were also enriched in pathways related to lipid metabolism and hepatic damage. Collectively, these findings can be concluded that FA can alleviate early FLS by affecting lipogenesis, ER stress and apoptosis, which may be mediated by enhanced folate metabolism.

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ST8SIA6-AS1 promotes the development of hepatocellular carcinoma cells through miR-338-3p/NONO Axis

Cited by 9 publications

References 17 publications

Integrated analysis of multiple transcriptomic data identifies ST8SIA6‑AS1 and LINC01093 as potential biomarkers in HBV‑associated liver cancer

Integrated analysis of multiple transcriptomic data identifies ST8SIA6‑AS1 and LINC01093 as potential biomarkers in HBV‑associated liver cancer

LncRNA ST8SIA6-AS1 facilitates hepatocellular carcinoma progression by governing miR-651-5p/TM4SF4 axis

Functional roles of folic acid in alleviating dexamethasone‐induced fatty liver syndrome in laying hens

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