SSU rDNA analysis of Kudoa rosenbuschi (Myxosporea) from the Argentinean hake Merluccius hubbsi

Abollo, Elvira; Novoa, Beatriz; Figueras, Antonio

doi:10.3354/dao064135

Cited by 18 publications

(10 citation statements)

References 12 publications

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“…However, the resemblance of K. alliaria spores (Fig. 1) to those of K. rosenbuschi provided in figures by Sardella (1988) and Abollo et al (2005) is striking. This is not to say that these authors made a misidentification, but simply illustrates some of the morphological similarities between these parasites.…”

Section: Resultsmentioning

confidence: 80%

“…This sequence was compared to those of other related Kudoa species as determined from previous analyses (Blaylock et al 2004, Abollo et al 2005, Diamant et al 2005) and using the basic local alignment search tool (BLAST) on GenBank (Altschul et al 1990). Sequences were aligned with Clustal X (Thompson et al 1997) and pairwise sequence similarities were generated with BioEdit version 7.0.5 (Hall 1999).…”

Section: Methodsmentioning

confidence: 99%

“…Given that myxozoan spores, when compared to other metazoan phyla, possess relatively few features from which identifications and descriptions can be made, it has been suggested that molecular characterizations should accompany descriptions of new species (Kent et al 2001). Recently, Abollo et al (2005) sequenced the small subunit ribosomal DNA (SSU rDNA) gene of K. rosenbuschi. Based on a phylogentic analysis of this sequence, Abollo et al (2005) found that K. rosenbuschi was closely related to the morphologically similar parasites K. clupeidae and K. funduli.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Abollo et al (2005) sequenced the small subunit ribosomal DNA (SSU rDNA) gene of K. rosenbuschi. Based on a phylogentic analysis of this sequence, Abollo et al (2005) found that K. rosenbuschi was closely related to the morphologically similar parasites K. clupeidae and K. funduli. In 2002, we received specimens of Argentinian hoki Macruronus magellanicus infected with a Kudoa species.…”

Section: Introductionmentioning

confidence: 99%

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Kudoa alliaria in flesh of Argentinian hoki Macruronus magellanicus (Gadiformes; Merlucciidae)

Whipps¹,

Diggles²

2006

Dis. Aquat. Org.

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Section: Resultsmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Kudoa alliaria in flesh of Argentinian hoki Macruronus magellanicus (Gadiformes; Merlucciidae)

Whipps¹,

Diggles²

2006

Dis. Aquat. Org.

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“…It has been reported that there are many Kudoa spp. and that they infect both freshwater fish and sea fish (Abollo et al 2005); however, only K. septempunctata has been proposed to be detrimental to human health. One characteristic of the foodborne sickness caused by K. septempunctata is a rapid recovery, and the disease has a good prognosis ).…”

Section: Introductionmentioning

confidence: 99%

Kudoa septempunctatawas recognised by Toll-like receptor 2 produced by a RAW 264 macrophage-like cell line

Ohnishi

Oyama

Furusawa

et al. 2013

Food Additives & Contaminants: Part A

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Kudoa septempunctata is a myxosporean parasite that infects Paralichthys olivaceus (olive flounder). Previously, we reported that the consumption of raw P. olivaceus meat containing a high concentration of K. septempunctata spores induces transient but severe diarrhoea and emesis. In this study, we investigated the cytokine production of mouse macrophage-like RAW 264 cells stimulated with K. septempunctata. When the RAW 264 cells were incubated with the spores of K. septempunctata for 24 h, they secreted tumour necrosis factor α (TNF-α) and several chemokines, such as IP-10, MIP-1β, and MIP-2. The secretion of TNF-α was induced in a dose-dependent manner in a bioassay using L929 cells and mouse TNF-α-specific enzyme-linked immunosorbent assay (ELISA). To identify the macrophage receptor of K. septempunctata, activation of HEK 293 cells expressing one of the Toll-like receptors (TLR) was measured using an NF-κB-dependent reporter assay. TLR2-expressing HEK 293 cells were strongly activated following stimulation with the spores. These results suggested that K. septempunctata was recognised by TLR2 on the macrophages, which were then activated and produced TNF-α.

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Characterization of the ribosomal RNA gene of Kudoa neothunni (Myxosporea: Multivalvulida) in tunas (Thunnus spp.) and Kudoa scomberi n. sp. in a chub mackerel (Scomber japonicus)

Sato

Tanaka

et al. 2013

Parasitol Res

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Kudoa neothunni is the first described Kudoa species having six shell valves and polar capsules, previously assigned to the genus Hexacapsula Arai and Matsumoto, 1953. Since its genetic analyses remain to be conducted, the present study characterizes the ribosomal RNA gene (rDNA) using two isolates from a yellowfin tuna (Thunnus albacares) with post-harvest myoliquefaction and a northern bluefin tuna (Thunnus thynnus) without tissue degradation. Spores of the two isolates localized in the myofiber of trunk muscles, forming pseudocysts, and showed typical morphology of K. neothunni with six equal-sized shell valves radially arranged in apical view: spores (n = 15) measuring 9.5-11.4 μm in width, 7.3-8.6 μm in suture width, 8.9-10.9 μm in thickness, and 7.3-7.7 μm in length; and polar capsules measuring 3.6-4.1 μm by 1.8-2.3 μm. In lateral view, the spores were pyramidal in shape without apical protrusions. Their 18S and 5.8S rDNA sequences were essentially identical, but variations in the ITS1 (62.4 % similarity across 757-bp length), ITS2 (66.9 % similarity across 599-bp length), and 28S (99.0 % similarity across 2,245-bp length) rDNA regions existed between the two isolates. On phylogenetic trees based on the 18S or 28S rDNA sequence, K. neothunni formed a clade with Kudoa spp. with more than four shell valves and polar capsules, particularly K. grammatorcyni and K. scomberomori. Semiquadrate spores of a kudoid species with four shell valves and polar capsules were detected from minute cysts (0.30-0.75 mm by 0.20-0.40 mm) embedded in the trunk muscle of a chub mackerel (Scomber japonicus) fished in the Sea of Japan. Morphologically, it resembled K. caudata described from a chub mackerel fished in the southeastern Pacific Ocean off Peru; however, it lacked filamentous projections on the shell valves of spores. Additionally, it morphologically resembled K. thunni described from a yellowfin tuna also fished in the Pacific Ocean; spores (n = 30) measuring 8.2-10.5 μm in width, 7.0-8.8 μm in thickness, and 6.1-6.8 μm in length; and polar capsule measuring 2.5-3.4 μm by 1.3-2.0 μm. The similarities of the 18S and 28S rDNA sequences between these two species were 98.5 % and 96.3 %, respectively. Simultaneously, the dimensions of cysts in the trunk muscle formed by K. thunni are clearly larger than those of the present species from a chub mackerel: 1.3-2.0 mm by 1.1-1.4 mm (n = 14) vs. 0.30-0.75 mm by 0.20-0.40 mm (n = 7), respectively. Thus, Kudoa scomberi n. sp. is proposed for this multivalvulid species found in the chub mackerel.

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SSU rDNA analysis of Kudoa rosenbuschi (Myxosporea) from the Argentinean hake Merluccius hubbsi

Cited by 18 publications

References 12 publications

Kudoa alliaria in flesh of Argentinian hoki Macruronus magellanicus (Gadiformes; Merlucciidae)

Kudoa alliaria in flesh of Argentinian hoki Macruronus magellanicus (Gadiformes; Merlucciidae)

Kudoa septempunctatawas recognised by Toll-like receptor 2 produced by a RAW 264 macrophage-like cell line

Characterization of the ribosomal RNA gene of Kudoa neothunni (Myxosporea: Multivalvulida) in tunas (Thunnus spp.) and Kudoa scomberi n. sp. in a chub mackerel (Scomber japonicus)

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