ssRNA base pairing at a bilayer interface can be controlled by the acyl chain order

Michanek, Agnes; Nylander, Tommy; Sparr, Emma; Björklund, Mathias

doi:10.1039/c2sm06700e

Cited by 8 publications

(8 citation statements)

References 58 publications

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“…The two-regime behaviour observed for the adsorption of TO3-4 and TS3 indicates that, at low surface coverage, these RNA structures adsorb as a thin layer comparable to that observed for linear RNA molecules (e.g. RNA 9-10 and previous studies 36 ). When the number of RNA structures at the surface increases, the surface layer starts to protrude into the bulk and there is room for more RNA assemblies at the surface.…”

Section: Qcm-d Probes the Protrusion Of Rna Nanostructures From The B...supporting

confidence: 71%

“…The small shift in both Δ F and Δ D observed for the RNA 9-10 , indicates that the oligomer complexes adsorb to form a flat and thin layer at the surface, consistent with previous observations of adsorbed layers of other linear RNA and DNA. 27,36 In contrast, the large changes in both Δ F and Δ D observed during adsorption of the TO3-4 polyhedrons and the TS3 and TS4 RNA tectosquares onto the bilayer, imply that there is a significant amount of coupled water associated with the RNA structures, and that the adsorbed structures retain their 3D shapes. Thus, the RNA assemblies are not an integral part of the bilayer but rather form a protruding layer.…”

Section: Resultsmentioning

confidence: 99%

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Assembly of RNA nanostructures on supported lipid bilayers

et al. 2015

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The assembly of nucleic acid nanostructures with controlled size and shape has large impact in the fields of nanotechnology, nanomedicine and synthetic biology. The directed arrangement of nanostructures at interfaces is important for many applications. In spite of this, the use of laterally mobile lipid bilayers to control RNA three-dimensional nanostructure formation on surfaces remains largely unexplored. Here, we direct the self-assembly of RNA building blocks into three-dimensional structures of RNA on fluid lipid bilayers composed of cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or mixtures of zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) and cationic sphingosine. We demonstrate the stepwise supramolecular assembly of discrete building blocks through specific and selective RNA-RNA interactions, based on results from quartz crystal microbalance with dissipation (QCM-D), ellipsometry, fluorescence recovery after photobleaching (FRAP) and total internal reflection fluorescence microscopy (TIRF) experiments. The assembly can be controlled to give a densely packed single layer of RNA polyhedrons at the fluid lipid bilayer surface. We show that assembly of the 3D structure can be modulated by sequence specific interactions, surface charge and changes in the salt composition and concentration. In addition, the tertiary structure of the RNA polyhedron can be controllably switched from an extended structure to one that is dense and compact. The versatile approach to building up three-dimensional structures of RNA does not require modification of the surface or the RNA molecules, and can be used as a bottom-up means of nanofabrication of functionalized bio-mimicking surfaces.

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Section: Qcm-d Probes the Protrusion Of Rna Nanostructures From The B...supporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Assembly of RNA nanostructures on supported lipid bilayers

et al. 2015

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“…This seems to indicate that the single stranded nature of the structure is important to allow this penetration into the lipid layer, which is possibly due to the less bulky nature of the NA under these conditions and/or that such a configuration allows access to the more hydrophobic parts of the nucleotides promoting hydrophobic interactions with the lipid acyl chains, as proposed previously. [74][75][76] Although the model fitting of the NR data for the polyU is somewhat ambiguous, the presence of the broad peak in Fig. 7 indicates multilayer formation.…”

Section: Interaction Between Nucleic Acids and The Lipid Layermentioning

confidence: 99%

On the interactions between RNA and titrateable lipid layers: implications for RNA delivery with lipid nanoparticles

Gilbert,

Ermilova,

Fornasier

et al. 2024

Nanoscale

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“…Supported lipid bilayers are versatile substrates that can be used to emulate the biomembrane interactions of proteins, 1,2 cells, 3 polymer microgels, 4 vesicles 5 and nucleic acids. 6,7 Such systems can be used not only to simulate and study processes at a cell membrane surface, but also to construct biomimetic systems for e.g. bioanalytical purposes.…”

Section: Introductionmentioning

confidence: 99%

Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates

et al. 2015

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The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.

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ssRNA base pairing at a bilayer interface can be controlled by the acyl chain order

Cited by 8 publications

References 58 publications

Assembly of RNA nanostructures on supported lipid bilayers

Assembly of RNA nanostructures on supported lipid bilayers

On the interactions between RNA and titrateable lipid layers: implications for RNA delivery with lipid nanoparticles

Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates

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