SSR markers associated with fertility restoration genes against Triticum timopheevii cytoplasm in Triticum aestivum

Abstract

Heterosis is an important way to improve yield and quality for many crops. Hybrid rice and hybrid maize contributed to enhanced productivity which is essential to supply enough food for the increasing world population. The success of hybrid rice in China has led to a continuous interest in hybrid wheat, even when most research on hybrid wheat has been discontinued in other countries for various reasons including low heterosis and high seed production costs. The Timopheevii cytoplasmic male sterile system is ideal for producing hybrid wheat seeds when fertility restoration lines with strong fertility restoration ability are available. To develop PCR-based molecular markers for use in marker-assisted selection of fertility restorer lines, two F 2 populations derived from crosses R18/ND36 and R9034/ND36 were used to map fertility restoration genes in the two elite fertility restorer lines (R-lines) R18 and R9034. Over 678 SSR markers were analyzed, and markers closely linked to fertility restoration genes were identified. Using SSR markers, a major fertility restoration gene, Rf3, was located on the 1B chromosome in both populations. This gene was partially dominant in conferring fertility restoration in the two restorer lines. SSR markers Xbarc207, Xgwm131, and Xbarc61 are close to this gene. These markers may be useful in marker-assisted selection of new restorer lines with T. timopheevii cytoplasm. Two minor QTL conferring fertility restoration were also identified on chromosomes 5A (in R18) and 7D (in R9034) in two R-lines.

Identification of Rf9, a Gene Contributing to the Genetic Complexity of Fertility Restoration in Hybrid Wheat

“…This indicates that Rf3 was not present in BC 1 Gerek 79 or BC 1 71R1203. A comparison of flanking SNP markers and genetic distances also showed that none of these minor QTL was linked to a region associated with a major Rf gene previously detected (Ma et al, 1995;Ahmed et al, 2001;Zhou et al, 2005;Stojałowski et al, 2013).…”

“…Composite interval mapping also detected minor QTL QRf.lfl-4AL, QStS.lfl-2AL, and QStS.lfl-6BS in BC 1 Gerek 79 and QRf.lfl-1BS, QRf.lfl-5BL, and QStS.lfl-1DS in BC 1 71R1203 ( Table 2). The two QTL on chromosomes 1BS and 6BS may be identical to previously identified minor QTL (Ahmed et al, 2001;Zhou et al, 2005;Geyer et al, 2018). A KASP assay developed based on the SNP IWB72107 was used to determine whether QTL QRf.lfl-1BS could be associated with Rf3 located on chromosome 1BS (Geyer et al, 2018).…”

A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)

“…The mapping analysis indicated that the male sterile gene wptms3 located on chromosome 1B, flanked by Xgwm413 and Xgwm182, differed from those reported by Guo et al (2006b) and thus is a new gene. To date, several fertility restoring genes against CMS in wheat have been mapped on chromosome 1B (Ahmed et al, 2001;Li et al, 2005;Zhou et al, 2005). Therefore, there are regions on chromosome 1B related to fertility performance.…”

“…It has been widely utilized to improve yield and quality in many crops such as maize, rice, sorghum, oilseed rape, and cotton. The production of hybrid seeds is undertaken using the following systems: mechanical emasculation, chemical hybridizing agent (CHA), and application of male sterility (Zhou et al, 2005). Due to the laborious work of mechanical emasculation and difficulty in the right usage of CHA, the male sterile system is preferred by breeders.…”

Application of LW7 marker for identification of progenies with male sterility gene in sweet sorghum population

“…DNA-based molecular markers tightly linked to fertility restorer genes have also been identified in several other species such as rice (Akagi et al 1996, Ichikawa et al 1997, Zhang et al 1997, petunia (Bentolila et al 1998, Bentolila andHanson 2001), barley (Matsu et al 2001), sunflower (Horn et al 2003, cotton (Gossypium hirsutum; Liu et al 2003), wheat (Zhou et al 2005), chili pepper (Kim et al 2006) and sorghum (Klein et al 2001, Jordan et al 2010, Jordan et al 2011. In sweet sorghum, DNA markers associated with several morphological traits (Shehzad et al 2009), agronomic traits (Yun-long et al 2006, Ritter et al 2008) and stem sugar-related traits (Ritter et al 2008, Shiringani et al 2010) have been identified and mapped into sorghum genetic linkage map (Ritter et al 2007, Srinivas et al 2009 (Klein et al 2001); SSR Xtxp304 (Jordan et al 2010); LW7; LW8; and LW9 marker (Wen et al 2002).…”