SSR-based population structure, molecular diversity and linkage disequilibrium analysis of a collection of flax (Linum usitatissimum L.) varying for mucilage seed-coat content

Soto‐Cerda, Braulio J.; Maureira-Butler, Iván J.; Muñoz, Gastón; Rupayan, Annally; Cloutier, Sylvie

doi:10.1007/s11032-011-9670-y

Cited by 40 publications

(39 citation statements)

References 69 publications

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“…In AMOVA the F ST value was observed 0.21 supporting strong population structures. Our results were found to be similar to those reported earlier (Soto-Cerda et al 2012). The high broad sense heritability of days to 50% flowering and plant height and association among these traits suggests that breeding for early flowering and shorter plant type is feasible.…”

Section: Discussionsupporting

confidence: 90%

Genetic diversity, population structure and association analysis in linseed (Linum usitatissimum L.)

Chandrawati

Singh

Kumar³

et al. 2017

Physiol Mol Biol Plants

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The present investigation aimed to explore the level of genetic diversity, determine the population structure in a larger set of germplasm of linseed using microsatellite marker and identify linked markers through association mapping. A total of 168 accessions of linseed were evaluated for major agro-economic traits and SSRs markers deployed for diversity assessment. A total of 337 alleles were amplified by 50 SSRs ranging from 2 to 13 with an average of 6.74 ± 2.8 alleles per loci. The neighbor joining based clustering grouped all the accessions into three major clusters that were also confirmed by scatter plot of PCoA. While model based clustering determined four sub-populations (K = 4). Further, analysis of molecular variance analysis considering three population showed that maximum variation (79%) was within the population. We identified one putative SSR marker (Lu_3043) linked with days to 50% flowering through both GLM and MLM analysis of association mapping. The results of this preliminary study revealed genetic diversity, population structure in linseed and linked marker which could be utilized in future breeding program.

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Section: Discussionsupporting

confidence: 90%

Genetic diversity, population structure and association analysis in linseed (Linum usitatissimum L.)

Chandrawati

Singh

Kumar³

et al. 2017

Physiol Mol Biol Plants

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“…Comparisons between cultivars, landraces and wild relatives have been reported based on different marker systems. These studies showed a lower genetic diversity in cultivated flax compared to that of its wild relatives or landraces (Soto-Cerda et al 2012).…”

Section: Discussionmentioning

confidence: 96%

“…The center of origin of cultivated flax is believed to be the Middle East, although other secondary diversity centers have been proposed such as the Mediterranean basin, Ethiopia, Central Asia and India (Zohary and Hopf 2000). Flax is considered a multi-purpose crop because of its straw fiber, its seed that is rich in high linolenic acid and antioxidants and its soluble fiber present in the seed coat (in the form of mucilage, Soto-Cerda et al 2012). L. usitatissimum can hybridize with closely related species having a similar ploidy level, genome, and chromosome pairing (Jhala et al 2008).…”

Section: Introductionmentioning

confidence: 99%

SSR and EST-SSR-based population genetic structure of Linum L. (Linaceae) species in Iran

Habibollahi

Noormohammadi

Sheidai

et al. 2015

Genet Resour Crop Evol

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The genus Linum L. (Linaceae) has more than 200 species. These species have agricultural, industrial, and medicinal importance. Domestication and continuous selection reduce genetic diversity of plants and in order to develop new and improved varieties, we should utilize all available genetic diversity in that crop and its wild relatives. The cultivated flax (L. usitatissimum L.) is an important crop plant and is being evaluated as a crop platform for the production of bio-industrial and nutraceutical products. In present study, we studied 230 plants of 23 populations in four Linum species namely, pale flax (L. bienne Mill.), cultivated flax (L. usitatissimum L.), and two wild relatives, L. austriacum L. and L. album L. The objective of this study was to investigate population genetic structure and genetic variability within each species using SSR and EST-SSR markers.The low degree of genetic variability was obtained in these species. AMOVA revealed significant genetic difference among species and populations. The significant correlation was observed between genetic and geographical distances the studied species. Evanno test and K-Means clustering revealed genetic fragmentation in the studied Linum species. Networking and STRUCTURE analysis revealed genetic affinity of the populations. The population assignment test revealed absence of gene flow or the occurrence of very limited gene flow among populations.

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“…The GD and PIC from the present study showed the presence of high GD and PIC in Ethiopian linseed accessions as compared to the results obtained for samples collected from different countries (SotoCerda et al 2012(SotoCerda et al : Ž iarovská et al 2012. '' Soto-Cerda et al (2012) used a core set of linseed germplasm developed from 60 accessions as representative of 16 countries from three continents and obtained 0.34 mean value of GD from 150 microsatellite (85 gSSR and 65 EST-SSR). Ž iarovská et al (2012) reported PIC values from four ISSR and two IRAP primers ranged from 0.12 to 0.37 and from 0.22 to 0.28, respectively from 18 accessions collected from 11 countries.…”

Section: Discussionmentioning

confidence: 99%

Biodiversity in Ethiopian linseed (Linum usitatissimum L.): molecular characterization of landraces and some wild species

Mhiret

Heslop‐Harrison

2018

Genet Resour Crop Evol

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Molecular characterization of germplasm is important for sustainable exploitation of crops. DNA diversity was measured using inter-retrotransposon-amplified-polymorphism and inter-simple-sequence-repeat markers in 203 Ethiopian landraces and reference varieties of linseed (flax, Linum usitatissimum) and wild Linum species. Molecular diversity was high (PIC, 0.16; GD, 0.19) compared to other reports from the species. Genotyping separated reference from landrace accessions, and clustered landrace accessions from different altitudes and geographical regions. Collections showed evidence for recent introduction of varieties in some regions. The phylogeny supported L. bienne Mill. as the progenitor of domesticated L. usitatissimum. Markers developed here will be useful for genetic mapping and selection of breeding lines. The results show the range of characters that can be exploited in breeding lines appropriate for smallholder and commercial farmers in Ethiopia, producing a sustainable, secure, highvalue crop meeting agricultural, economic and cultural needs.

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SSR-based population structure, molecular diversity and linkage disequilibrium analysis of a collection of flax (Linum usitatissimum L.) varying for mucilage seed-coat content

Cited by 40 publications

References 69 publications

Genetic diversity, population structure and association analysis in linseed (Linum usitatissimum L.)

Genetic diversity, population structure and association analysis in linseed (Linum usitatissimum L.)

SSR and EST-SSR-based population genetic structure of Linum L. (Linaceae) species in Iran

Biodiversity in Ethiopian linseed (Linum usitatissimum L.): molecular characterization of landraces and some wild species

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