SSR analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories

Ignjatović-Micić, Dragana; Mladenovic-Drinic, Snezana; Nikolić, Ana; Lazic-Jancic, Vesna

doi:10.1134/s1022795408110100

Cited by 12 publications

(3 citation statements)

References 18 publications

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“…The mean number of alleles per locus within accessions in the Uruguayan white dent collection (A = 2.92) was larger than in other landraces sets collected outside of the maize center of origin, such as the Sahara (Aci et al, 2013), Switzerland (Eschholz et al, 2010), and former Yugoslavia (Ignjatović-Micić et al, 2008). In contrast, greater values of A were found in maize landraces from Sinaloa, Mexico (Pineda-Hidalgo et al, 2013), and in a collection from the Andean region, Chile (Salazar et al, 2017).…”

Section: Diversity Within Accessionsmentioning

confidence: 72%

Genetic Structure, Core Collection, and Regeneration Quality in White Dent Corn Landraces

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Franco³

et al. 2018

Crop Science

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Genetic diversity and structure of plant germplasm collections, frequently determined by molecular markers, can be used to assist breeding programs, to validate core collections determined by other methodologies, to identify priority accessions for conservation, and to confirm genetic integrity after regeneration. This research aimed to study the genetic diversity and structure of the Uruguayan white dent maize collection, to evaluate the genetic representativeness of its core collection (previously defined by phenotypic traits), and to confirm the genetic integrity of seven regenerations made in Mexico and Uruguay in comparison with the original accessions. Ninety accessions were fingerprinted using 26 simple sequence repeat (SSR) markers. Genetic structure was analyzed by Ward clustering, canonical analysis, and a Bayesian approach based on allelic frequencies. All SSR markers were polymorphic with a mean number of alleles (A) of 7.43, an effective allele number (Ae) of 3.04, and expected heterozygosity of 0.579. The genetic variation between accessions was 0.251, and variation within accessions was 0.749. Four genetic groups were obtained using the three approaches. The core collection represented the structure of the whole collection because the four genetic groups were proportionally represented. The genetic diversity in the core collection did not differ from the entire collection in A, Ae, expected heterozygosity (He) percentage of polymorphic loci (%P), and expected heterozygosity within accessions (Hs). Most regenerations (9 out of 14) preserved the genetic integrity of original accessions, whereas in other cases, either new or lost alleles caused genetic differences. The analysis of genetic structure and diversity of germplasm collections, in combination with morphological characterizations, helps to define ex situ conservation strategies and usage in breeding programs.

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Section: Diversity Within Accessionsmentioning

confidence: 72%

Genetic Structure, Core Collection, and Regeneration Quality in White Dent Corn Landraces

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Condón

Franco³

et al. 2018

Crop Science

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“…Sharma et al (2010) reportaron el mayor número de alelos por locus (13), hasta donde se tiene conocimiento, al estudiar 48 accesiones de la región de la India del Estado de Sikkim, con 42 SSRs. En estudios realizados de germoplasma en la ex-Yugoslavia se han reportado valores de solo 2.5 alelos por locus al utilizar 25 SSRs en solo 21 poblaciones (Ignjatović-Micić et al, 2008).…”

Section: Caracterización De La Diversidad Genéticaunclassified

Diversidad Genética en Maíces Nativos Mexicanos Tropicales

et al. 2013

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México es considerado como el centro de origen y domesticación del maíz (Zea mays L.) y uno de los centros más reconocidos de su diversidad. La evaluación de la diversidad en maíces nativos es importante para el planteamiento de estrategias de conservación, caracterización y uso del germoplasma en el mejoramiento genético, dado su potencial como fuente de características nuevas, exóticas y favorables. En este estudio se utilizaron 30 marcadores moleculares tipo microsatélite con el objetivo de caracterizar la diversidad genética interpoblacional e intrarracial presente en 196 poblaciones del trópico de México representativas de 20 razas de maíz y provenientes de 21 estados de la República Mexicana. Los resultados obtenidos indican que dichas accesiones pueden ser agrupadas en tres áreas ecológicas: Maíces del Golfo de México, Pacífico Sur y Península de Yucatán (A), Maíces de la Zona Noroeste y Occidente (B), y Maíces de Tierras Bajas e Intermedias de Oaxaca y Chiapas (C). A través de las 196 poblaciones se encontró un promedio de 9 alelos por locus y una diversidad genética promedio de 0.57. Se encontró mayor variabilidad entre razas (23.18) que dentro de cada raza (0.99 a 8.72). En razas como Jala, Zapalote Grande y Zapalote Chico, se evidenció la erosión genética debido a su limitada distribución geográfica, lo que plantea la necesidad de dedicar esfuerzos a su conservación. Índices de diversidad genética de 0.53 encontrados para los Tuxpeños corroboran que pese a que ha sido ampliamente utilizado en los programas de mejoramiento, aún hay amplio potencial genético por explorar en esta raza.

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“…In the first study 21 landraces belonging to seven different agro ecological groups have been subjected to SSR analysis with aim to develop genetic fingerprint for characterization, identification and classification of the landraces, as well as for estimation of their genetic diversity (Ignjatovic-Micic et al, 2008). In the second study eighteen landraces from six different agro-ecological groups were assayed with AFLP and SSR markers (Ignjatovic-Micic et al, 2007).…”

Section: Molecular Marker Analysismentioning

confidence: 99%