SsNAC23, a member of the NAC domain protein family, is associated with cold, herbivory and water stress in sugarcane

Nogueira, Fábio Tebaldi Silveira; Schlögl, Paulo Sérgio; Camargo, Sandra R.; Fernández, Jorge Hernández; Rosa, Vicente E. De; Pompermayer, Patrícia; Arruda, Paulo

doi:10.1016/j.plantsci.2005.03.008

Cited by 78 publications

(56 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nogueira et al (2005) showed that expression of SsNAC23 is induced in sugarcane plantlets cultivated in vitro and exposed to 4ºC for 48 h. Thus, SsNAC23 cold induction is not an artifact of in vitro cultivation or a specific phenomenon for a particular sugarcane variety, confirming its significance. Previously, it was demonstrated that SsNAC23 contains a degenerate bipartite nuclear localization signal (NLS), conserved in other NAC protein domain, and that it is localized in the nucleus of onion epidermal cells (Nogueira et al, 2005), further supporting its role as a transcription factor. Regarding tissue expression, SsNAC23 was found to be present in leaves and roots at high to moderate levels, in stems at lower levels, and undetected in the apical meristem and floral organs by northern hybridization (Nogueira et al, 2005).…”

Section: Resultsmentioning

confidence: 71%

“…cv. SP80-3280), propagated axenically in vitro, were maintained as described previously (Nogueira et al, 2005). Plants from the variety Q117 were grown under greenhouse conditions in 200 mL plastic cups containing a commercial planting mix plus ammonium sulfate and commercial 4-20-20 fertilizer.…”

Section: Methodsmentioning

confidence: 99%

“…The RNA was separated by electrophoresis in a formaldehyde agarose (1%) gel and transferred to nylon membranes (Hybond N, Amersham Bio-sciences, USA). The blots were hybridized with a conserved 3'-end fragment of SsNAC23, as described previously (Nogueira et al, 2005).…”

Section: Methodsmentioning

confidence: 99%

“…To allow a better understanding of the oxidized and reduced forms of SsNAC23 dimeric structure, a molecular model already described (Nogueira et al, 2005) was submitted for additional energy minimization and equilibrating molecular dynamics simulations. To this end, the Gromacs 4.0.2 program (Van Der Spoel et al, 2005) was used on a quadcore Linux workstation using the GROMOS 53a6 force field.…”

Section: Methodsmentioning

confidence: 99%

“…Modeled SsNAC23 dimer (as described in Nogueira et al, (2005)) was solvated in a water box using the SPC/E model (Brunne et al, 1993). Reduced and oxidized topologies of the protein were obtained using the pdb2gmx tool.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

Ditt

Gentile

Tavares

et al. 2011

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a coldsensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC). With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.

show abstract

Section: Resultsmentioning

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

Ditt

Gentile

Tavares

et al. 2011

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

show abstract

Biochemistry and Molecular Biology in Fruits during Cold Storage

Cainelli

Ruperti

2019

Annual Plant Reviews Online

View full text Add to dashboard Cite

Cold storage of fruits is exploited to prolong postharvest life and marketing period by minimising their metabolic activity. However, exposure of fruits to nonfreezing low temperatures for prolonged periods of time may also lead to the development of cold‐inducible physiological disorders termed chilling injuries. Chilling injuries represent an economically relevant problem that may occur in an unpredictable way depending on the sensitivity of the fruits to low temperature. In this article, we consider the main aspects of cold stress signalling and responses taking place in fruits during storage and during the following period of warming at room temperature that often results in the further induction of chilling injury symptoms. A vast amount of research has contributed to the identification of several biochemical and molecular events underlying cold stress responses in fruits. Convincing evidence shows that ethylene plays a pivotal regulatory role, through a complex interplay with other hormones, but early inducing factors remain elusive. Recent studies based on system analyses of cold‐dependent trancriptomic and metabolomic changes have contributed to shed light on some early regulatory events which may contribute to determining the fruits' sensitivity to cold stress. A better understanding of these phenomena is essential to optimise cold storage conditions and for an efficient use of resources to deliver a more sustainable fruit supply chain.

show abstract

Role of Transcription Factors in Abiotic Stress Tolerance in Crop Plants

Yadav¹,

Rani²,

Aneja³

et al. 2013

Climate Change and Plant Abiotic Stress Tolerance

View full text Add to dashboard Cite

Plants are continuously exposed to a myriad of abiotic stresses that limit plant growth and productivity due to altered physiological and molecular responses. Considerable efforts have been made to identify abiotic stress-induced genes and to establish whether they have a role in stress tolerance. The stress responses of plants are regulated by multiple signaling pathways and there is significant overlap between the patterns of gene expression that are induced in plants in response to different stresses. Stress-related genes are induced primarily at the level of transcription, and regulating the temporal and spatial expression patterns of specific stress genes is an important part of the plant stress response. cDNA microarrays and genomics approaches reveal a clear overlap in the genes expressed in response to different stresses. Responses and adaptation under abiotic stress require differential gene expression, which is regulated by specific transcription factors. Transcription factors are proteins with a DNA domain that binds to the cisacting element present in the promoter of a target gene. This chapter gives an overview of the role of important plant transcription factors that control the expression of multiple target genes involved in abiotic stress tolerance. ABRE, MYC/MYB, CBF/DREB, AP2/ERF, and NAC are major plant-specific families of transcription factors that regulate abiotic stress-induced multiple gene expression in an abscisic acid-dependent or -independent manner. Application of forward and reverse genetic analysis and the growing power of genomics and proteomics has accelerated our knowledge about the complex role of transcription factors in stress tolerance mechanisms. Since transcription factor genes have the potential for the coordinated regulartion of stress-induced genes relevant to stress tolerance, there is a growing interest in the use of regulatory genes for developing abiotic stresstolerant genotypes.

show abstract

SsNAC23, a member of the NAC domain protein family, is associated with cold, herbivory and water stress in sugarcane

Cited by 78 publications

References 49 publications

Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

Biochemistry and Molecular Biology in Fruits during Cold Storage

Role of Transcription Factors in Abiotic Stress Tolerance in Crop Plants

Contact Info

Product

Resources

About