SsHKT1;1 is coordinated with SsSOS1 and SsNHX1 to regulate Na+ homeostasis in Suaeda salsa under saline conditions

Wang, Wenying; Liu, Yaqi; Duan, Huirong; Yin, Xiu-Xia; Cui, Yan-Nong; Chai, Wei-Wei; Song, Xin; Flowers, T. J.; Wang, Suo-Min

doi:10.1007/s11104-020-04463-x

Cited by 40 publications

(38 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7). Secondly, NHX transporters facilitate the vacuolar sequestration of excess cytosolic Na + , which effectively avoids the accumulation of Na + in the cytoplasm [48]. In this study, ST 47 showed more signi cant up-regulation of genes related to the NHX transporter, compared with SS 212.…”

Section: Discussionmentioning

confidence: 55%

Unravelling the Strategies for Cell wall Biosynthesis used by Salt-Tolerant Proso Millet (Panicum miliaceum L.) under Salt Stress: From Root Structure to Molecular Mechanism

Yuan

Liu

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Considering the twin global problems of increasingly serious energy shortage and effects of salt stress on biofuel plants, breeding of salt-resistant biofuel plant and the discovery of mechanisms for biomass accumulation under salt stress is necessary for energy shortage. Proso millet (Panicum miliaceum L.) is very resilient to abiotic stress, especially to land degradation caused by soil salinization, and its promising as dedicated bioenergy crops for the production of renewable fuels and forage, due to its high photosynthetic efficiency C4 plant and ability to grow in a range of environmental conditions. However, the mechanisms by which the roots of proso millet adapt and tolerate salt-stress are obscure.Results: In this study, plants of a salt-sensitive cultivar (SS 212) and a salt-tolerant cultivar (ST 47) of proso millet were exposed to severe salt stress and subsequent re-watering. ST 47 exhibited greater salt tolerance and faster recovery than SS 212, as evidenced by higher increases in total root length (TRL), root surface area (RSA), root tip number (RTN), biomass. Moreover, microstructural analysis showed that relative to SS 212, the roots of ST 47 could maintain more intact internal structures, and thicker cell wall under salt stress, thereby stronger resistance to salt toxicity and maintenance of growth. Digital RNA sequence analysis suggested more genes involved in salt stress resistance were induced in ST 47 than in SS 212. In ST 47 also, re-watering restored most genes that had been induced by salt stress. Results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis revealed that ST 47 maintained better Na+/ K+ balance to resist Na+ toxicity via a higher capability to restrict Na+ uptake, vacuolar Na+ sequestration, and Na+ exclusion. The mechanism for cell wall biosynthesis in cultivar ST 47 involved the promotion of cell wall composition changes, via efficient regulation of galactose metabolism and biosynthesis of cellulose and phenylpropanoids. Conclusions: Overall, this study provides valuable salt-resistant biofuel resources and mechanisms for relieving the world energy shortage, which could be applied for the rehabilitation of saline lands.

show abstract

Section: Discussionmentioning

confidence: 55%

Unravelling the Strategies for Cell wall Biosynthesis used by Salt-Tolerant Proso Millet (Panicum miliaceum L.) under Salt Stress: From Root Structure to Molecular Mechanism

Yuan

Liu

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Had this occurred though, there would have been a reduction in the rate of Na + accumulation in the shoots. It has also been hypothesized that SOS1 in the roots functions by loading Na + into the xylem ( Zhang et al, 2017 ; Wang et al, 2020 ). Considering the converse activity of GhHKT1.A1 , the relative expression of GhSOS1.A12 was slightly higher in the roots of the tolerant genotypes under severe stress and may have resulted in both reduced Na + in the root and increased Na + in the shoot.…”

Section: Discussionmentioning

confidence: 99%

Networks of Physiological Adjustments and Defenses, and Their Synergy With Sodium (Na+) Homeostasis Explain the Hidden Variation for Salinity Tolerance Across the Cultivated Gossypium hirsutum Germplasm

et al. 2020

View full text Add to dashboard Cite

The abilities to mobilize and/or sequester excess ions within and outside the plant cell are important components of salt-tolerance mechanisms. Mobilization and sequestration of Na+ involves three transport systems facilitated by the plasma membrane H+/Na+ antiporter (SOS1), vacuolar H+/Na+ antiporter (NHX1), and Na+/K+ transporter in vascular tissues (HKT1). Many of these mechanisms are conserved across the plant kingdom. While Gossypium hirsutum (upland cotton) is significantly more salt-tolerant relative to other crops, the critical factors contributing to the phenotypic variation hidden across the germplasm have not been fully unraveled. In this study, the spatio-temporal patterns of Na+ accumulation along with other physiological and biochemical interactions were investigated at different severities of salinity across a meaningful genetic diversity panel across cultivated upland Gossypium. The aim was to define the importance of holistic or integrated effects relative to the direct effects of Na+ homeostasis mechanisms mediated by GhHKT1, GhSOS1, and GhNHX1. Multi-dimensional physio-morphometric attributes were investigated in a systems-level context using univariate and multivariate statistics, randomForest, and path analysis. Results showed that mobilized or sequestered Na+ contributes significantly to the baseline tolerance mechanisms. However, the observed variance in overall tolerance potential across a meaningful diversity panel were more significantly attributed to antioxidant capacity, maintenance of stomatal conductance, chlorophyll content, and divalent cation (Mg2+) contents other than Ca2+ through a complex interaction with Na+ homeostasis. The multi-tier macro-physiological, biochemical and molecular data generated in this study, and the networks of interactions uncovered strongly suggest that a complex physiological and biochemical synergy beyond the first-line-of defense (Na+ sequestration and mobilization) accounts for the total phenotypic variance across the primary germplasm of Gossypium hirsutum. These findings are consistent with the recently proposed Omnigenic Theory for quantitative traits and should contribute to a modern look at phenotypic selection for salt tolerance in cotton breeding.

show abstract

“…The accumulation of Na is largely restricted to salt-accumulation organs of halophytes. Some halophytes species, like Phragmites australis, can recirculate Na from the shoots back to the roots, keeping low Na concentrations in shoot vacuoles and high Na in root vacuoles (Wang et al 2020;Fujimaki et al 2015). Salt ions in salt-dilution halophytes accumulate in the vacuoles of succulent leaf tissues and other green tissues, as well as in the succulent column.…”

Section: The Effect Of Salt Tolerance Type On Element Concentrations In Different Plant Partsmentioning

confidence: 99%

“…Without Na, the health and development of halophytes cannot be guaranteed (Flowers and Colmer 2008). Na can regulate the osmotic pressure in plants, affecting their water balance and cell expansion (Wang et al 2020;Huang et al 2012). Another essential nutrient element is K (Zakery-Asl et al 2014).…”

Section: Introductionmentioning

confidence: 99%

The Element Composition Strategies in Chenopodiaceae Halophytes Reveal Complex Plant-Soil Interactions in Saline-Alkali Grassland

Song

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

With the continuous increase of saline-alkali land, sustainable development of the global environment and ecology has been seriously affected. Chenopodiaceae is a pioneer plant family living in saline-alkali land, which plays a crucial role in sustaining the stability, composition and function of saline-alkali arid ecosystems. In this study, 11 elements were analyzed in different plant organs and rhizosphere soil of eight Chenopodiaceae species. Descriptive statistics of the leaf, stem and root element concentrations in eight halophyte species of contrasting salt tolerance types (salt-dilution halophytes and recretohalophytes) were calculated. Spearman’s rank correlation coefficients were used to compare the correlations of elements among the leaf, stem, root and soil property, as well as between the plant and soil. General linear model was employed to explore how the variance of element concentrations in leaves, stems and roots depends on salt tolerance type, soil salinity property and soil mineral elements. The results showed that salt tolerance type and soil mineral element concentrations explained most of the variation observed in element concentrations in Chenopodiaceae plants; the soil salinity property played only a minor role. It was concluded that the genetic factors are the prerequisite in the composition pattern of leaf elements in Chenopodiaceae, and soil factors are the key to determine element accumulation. These conclusions provide an effective reference for evaluating plant breeding and its response to environmental change in saline-alkali arid areas in Hulunbuir grassland and other parts of the world.

show abstract

SsHKT1;1 is coordinated with SsSOS1 and SsNHX1 to regulate Na+ homeostasis in Suaeda salsa under saline conditions

Cited by 40 publications

References 78 publications

Unravelling the Strategies for Cell wall Biosynthesis used by Salt-Tolerant Proso Millet (Panicum miliaceum L.) under Salt Stress: From Root Structure to Molecular Mechanism

Unravelling the Strategies for Cell wall Biosynthesis used by Salt-Tolerant Proso Millet (Panicum miliaceum L.) under Salt Stress: From Root Structure to Molecular Mechanism

Networks of Physiological Adjustments and Defenses, and Their Synergy With Sodium (Na+) Homeostasis Explain the Hidden Variation for Salinity Tolerance Across the Cultivated Gossypium hirsutum Germplasm

The Element Composition Strategies in Chenopodiaceae Halophytes Reveal Complex Plant-Soil Interactions in Saline-Alkali Grassland

Contact Info

Product

Resources

About