SS18 Break-Apart Fluorescence In Situ Hybridization is a Practical and Effective Method for Diagnosing Microsecretory Adenocarcinoma of Salivary Glands

New entity of microsecretory adenocarcinoma of salivary glands: first case with recurrence and metastases — proof of malignancy

“…It exhibits uniform microcystic tubules with unilayered, cuboideal epithelium and PAS-positive luminal secretion and a consistent, but not specific immunophenotype (co-expression of CK14, CK18, S100, SOX10, p63 and negativity for p40, mammaglobin, and calponin) [1][2][3]. On molecular basis, MSA is characterized by an obviously obligate and specific MEF2C::SS18 gene fusion, which can be detected using RT-PCR or FISH [1,4,5].…”

Salivary gland polymorphous adenocarcinoma: Clinicopathological features and gene alterations in 36 Japanese patients

“…carefully revaluated and confirmed the histological diagnosis of PAC based on the 2017 WHO Classification of Head and Neck Tumors , and any discrepant results were discussed by an expert panel to achieve consensus. Other entities, such as myoepithelial carcinoma and microsecretory adenocarcinoma were eliminated from this study via ancillary testing, including immunohistochemistry for specific myoepithelial markers and SS18 fluorescence in situ hybridization (FISH), if necessary 16,17 . After excluding cases with poor‐quality DNA samples, 36 patients were enrolled for further clinicopathologic and molecular analyses.…”

Targeted RNA sequencing in the routine clinical detection of fusion genes in salivary gland tumors

“…Previously constructed tissue microarrays (TMAs) 43 containing 437 salivary gland tumors were investigated using immunohistochemistry (IHC) for MYB (clone: EP769Y; Abcam #ab45150); Pan‐Trk (clone: EPR17341; Abcam #ab181560); and, HMGA2 (polyclonal; Biocheck, 59170AP). Tissue sections were cut at 4 μm thickness and stained using a Dako OMNIS autostainer (Alignet, Santa Clara, California) as per the manufacturer's instructions.…”

“…Previously constructed tissue microarrays (TMAs) 43 containing 437 salivary gland tumors were investigated using immunohistochemistry (IHC) for MYB (clone: EP769Y; Abcam #ab45150);…”