Sry‐negative XX sex reversal in the American cocker spaniel dog

Meyers-Wallen, Vicki N.; Palmer, Victoria L.; Acland, G. M.; Hershfield, Bennett

doi:10.1002/mrd.1080410304

Cited by 48 publications

(31 citation statements)

References 29 publications

(55 reference statements)

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“…Coyote-derived faeces were sexed based on the presence of PCR product from the Y-chromosome SRY gene (Meyers-Wallen et al 1995). PCR of mtDNA and SRY used 50 ng of DNA isolated from blood or 5 ml of DNA extracted from faeces in a 20 ml reaction volume containing 1.0 mM MgCl 2 , 1.0 mg ml À1 bovine serum albumin (BSA), 0.05 mM of each dNTP, 5.0 pmoles of each primer, 1 ÂPCR reaction bu¡er and 0.3 units of Taq.…”

Section: (C) Faecal Typingmentioning

confidence: 99%

Estimating population size by genotyping faeces

Kohn

York

Kamradt

et al. 1999

Proc. R. Soc. Lond. B

435

445

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Population size is a fundamental biological parameter that is di¤cult to estimate. By genotyping coyote (Canis latrans) faeces systematically collected in the Santa Monica Mountains near Los Angeles, California, we exemplify a general, non-invasive method to census large mammals. Four steps are involved in the estimation. First, presumed coyote faeces are collected along paths or roadways where coyotes, like most carnivores, often defaecate and mark territorial boundaries. Second, DNA is extracted from the faeces and species identity and sex is determined by mitochondrial DNA and Y-chromosome typing. Third, hypervariable microsatellite loci are typed from the faeces. Lastly, rarefaction analysis is used to estimate population size from faecal genotypes. This method readily provides a point count estimate of population size and sex ratio. Additionally, we show that home range use, paternity and kinship can be inferred from the distribution and relatedness patterns of faecal genotypes.

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Section: (C) Faecal Typingmentioning

confidence: 99%

Estimating population size by genotyping faeces

Kohn

York

Kamradt

et al. 1999

Proc. R. Soc. Lond. B

435

445

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“…, Teppei KANDA 1) , Masashi NAGANO 2) , Akihiko SUGIYAMA 3) , Yoshiharu OKAMOTO 4) and Yoshiaki HIKASA 5) 1) Departments of Veterinary Internal Medicine, 2) Theriogenology, 3) Laboratory Medicine, 4) Veterinary Neurology and Oncology and 5) Veterinary ABSTRACT. A 10-year-old Miniature Schnauzer with bilateral cryptorchidism and male external genitalia was referred with a history of abdominal enlargement.…”

mentioning

confidence: 99%

“…To investigate the karyotype of the dog, chromosome preparations were obtained from a peripheral blood lymphocyte culture and stained with Giemsa [4]. Cytogenetic evaluation revealed a normal male chromosome compliment, 78, XY.…”

mentioning

confidence: 99%

A Case of Persistent Muellerian Duct Syndrome with Sertoli Cell Tumor and Hydrometra in a dog

Matsuu

Hashizume

Kanda

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. A 10-year-old Miniature Schnauzer with bilateral cryptorchidism and male external genitalia was referred with a history of abdominal enlargement. Upon exploratory laparotomy, two tumors and a connecting structure similar to fluid-filled uterus were recognized. After cytological and bacterial examinations of the fluid and histological examination, this dog was diagnosed with bilateral Sertoli cell tumor with hydrometra. The karyotype of this dog was 78, XY and the sry gene was detected positive by PCR. We diagnosed this dog as a case of persistent Müllerian duct syndrome (PMDS), which is male pseudohermaphroditism. This is the first report regarding the incidence of PMDS in Miniature Schnauzers in Japan, and it suggests the involvement of a gene carrier. A 10-year-old male Miniature Schnauzer was referred to Tottori University Animal Teaching Hospital with a history of abdominal enlargement. Physical examination revealed bilateral cryptorchidism with male external genitalia and non-pruritic dorsal lumbar alopecia. On palpation, a large mass was felt in the abdomen. The general condition seemed to be normal, and visible feminization was not recognized.Full CBC and serum biochemistry tests were performed. Slight elevations were observed in the levels of alanine amino transferase (182 U/l), alkaline phosphatase (1,363 U/ l), and total cholesterol (259 mg/dl). The concentrations of serum estradiol and testosterone were 103 pg/ml (reference range of normal male: < 15 pg/ml) and 246 ng/dl (reference range of normal male: 100-300 ng/dl), respectively. Abdominal radiographs detected large masses compressing the normal intestinal canal. Abdominal ultrasonography revealed two masses with mixed echogenecity. Additionally, hypoechogenecity tubular cysts cranial to the bladder were observed, which appeared to be fluid-filled like pyometra or hydrometra.Although the tubular cysts were unidentifiable, the abovementioned findings suggested the possibility of testicular tumors; thus, exploratory laparotomy was performed to explore the possibility of resecting the masses and cysts. A large and a small mass, which were speculated to represent the testicles, were located at the center of the abdomen and attached to the structure like the uterus which was inflated with fluid material. This uterus-like structure had two horns that caudally joined into the body of the uterus, the vagina terminated cranially into the prostate gland. Due to the growing mass, obvious structures like the fallopian duct or the testicular canal were not recognized. After the vagina was ligated, both the tumor and the uterus were extracted (Fig 1). Cytodiagnosis revealed intact erythrocyte and keratinizing epitheliums without pathogenic microorganisms. To identify the presence of aerobic bacteria, culture was performed and any multiplication of bacteria were not observed. The walls of both the uterine horns were thin. Histological examination confirmed bilateral Sertoli cell tumor and normal uterus; no ovarian tissue was found. The postoperative gen...

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“…In a previous report by Meyers-Wallen et (1999) a similar case was described but karyotypic confirmation of sex chromosomes was lacking. A retrospective study described a similar case (Meyers-Wallen et al 1995b), which indicated that three American Spaniel dogs had testicular tissue on the basis of gonadal histology but were negative for the presence of Sry based on PCR assays of genomic DNA. Although a large number of important genes are considered to be involved in testis differentiation, the genetic aetiology of SRY-negative XXSR in mammals is unexplained.…”

Section: Discussionmentioning

confidence: 99%

SRY-negative XX sex reversal in an English Cocker Spaniel: a case report

Nak¹,

Gulten²,

Karkucak³

et al. 2015

Vet. Med. - Czech

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A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosome which supports the hypothesis that some other genes are also responsible for testicular development. The present case report describes a one-year-old dog brought to the hospital with a complaint of no oestrus cycle. Apparently normal masculine bitch had an enlarged clitoris with an os on radiograph. A gonado-hysterectomy was performed. Instead of ovarian structures both testicular masses were present and a normal complete bicornuate uterus with a normal cervix was observed. The histology of both gonads showed two testes, each with its ductus deferens and the uterus with typical histological structure. Cytogenetic evaluation of peripheral blood lymphocytes revealed a female with a normal karyotype (78, XX). Polymerase chain reaction (PCR) analysis of genomic DNA showed that the SRY gene was absent.

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Sry‐negative XX sex reversal in the American cocker spaniel dog

Cited by 48 publications

References 29 publications

Estimating population size by genotyping faeces

Estimating population size by genotyping faeces

A Case of Persistent Muellerian Duct Syndrome with Sertoli Cell Tumor and Hydrometra in a dog

SRY-negative XX sex reversal in an English Cocker Spaniel: a case report

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