SRY-Box 21 Antisense RNA 1 Knockdown Diminishes Amyloid Beta25–35-Induced Neuronal Damage by miR-132/PI3K/AKT Pathway

Gu, Fengming; Ji, Daofei; Ni, Hongzao; Chen, Depeng

doi:10.1007/s11064-021-03373-3

Cited by 10 publications

(10 citation statements)

References 46 publications

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“…Approximately 2 × 10 5 transfected A549 and PC9 cells were seeded into each well of a 96-well plate, which was then placed in a 5% CO 2 incubator at 37°C for 48 h. The cells were lysed with a chilled lysis buffer provided with the kit for 10 min. Then, 50 μL of 2× reaction buffer containing 10 mM dithiothreitol and 5 μL of DEVD-p-NA substrate was added to the supernatant for 2 h at 37°C, followed by measurement of the absorbance at 400 nm on a microplate reader (Cat#: Elx808; Biotek, USA) [ 57 ].…”

Section: Methodsmentioning

confidence: 99%

Circular RNA (circ)_0129047 upregulates bone morphogenetic protein receptor type 2 expression to inhibit lung adenocarcinoma progression by sponging microRNA (miR)-1206

Xue

Chen

2022

Bioengineered

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Circular RNAs (circRNAs) play significant roles in the tumorigenesis and progression of various cancers, including lung adenocarcinoma (LAC). However, their underlying biological functions in LAC remain unclear. Here, we investigated the tumor suppressor role of the newly identified circRNA, circ_0129047, in LAC tumorigenesis and progression. The expression levels of circ_0129047, microRNA (miR)-1206, and bone morphogenetic protein receptor type 2 (BMPR2) mRNA in LAC cells and tissues were monitored using reverse transcription-quantitative polymerase chain reaction. Dual-luciferase reporter, RNA immunoprecipitation, and RNA pull-down assays were used to confirm the targeting relationships among circ_0129047, miR-1206, and BMPR2 mRNA. Functional experiments for A549 and PC9 cells were performed using cell counting kit-8, bromodeoxyuridine enzyme-linked immunosorbent, caspase-3 activity, cell adhesion, wound healing, and transwell assays. Circ_0129047 expression levels were reduced in LAC cells and tissues. Mechanistically, we discovered that circ_0129047 could sponge miR-1206, and miR-1206 could directly target BMPR2. In addition, circ_0129047 or BMPR2 knockdown facilitated the viability, proliferation, adhesion, migration, and invasion, while inhibiting the apoptosis of LAC cells. Furthermore, the inhibitory effects of circ_0129047 or BMPR2 overexpression on the malignant phenotype of LAC cells could be reversed by the overexpression of miR-1206. In conclusion, circ _0129047 was found to play a tumor suppressive role in LAC progression; it upregulated BMPR2 expression to inhibit LAC progression by sponging miR-1206. Abbreviations: non-small cell lung cancer (NSCLC); small cell lung cancer (SCLC); lung adenocarcinoma (LAC); Circular RNA (circRNA); MicroRNA (miRNA); bone morphogenetic protein (BMP); squamous cell lung cancer (SCC); RNA immunoprecipitation (RIP)

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Section: Methodsmentioning

confidence: 99%

Circular RNA (circ)_0129047 upregulates bone morphogenetic protein receptor type 2 expression to inhibit lung adenocarcinoma progression by sponging microRNA (miR)-1206

Xue

Chen

2022

Bioengineered

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“…LncRNA SOX21‐AS1 was upregulated in Aβ‐induced (Aβ 25‐35 ‐induced and Aβ 42 ‐induced) IMR‐32, SH‐SY5Y, and SK‐N‐SH cells 64,65 . SOX21‐AS1 acted as a sponge for miR‐107 and miR‐137 64,65 .…”

Section: Ncrnas Regulate Aβ‐induced Cell Apoptosis In Vitromentioning

confidence: 99%

NcRNAs: A synergistically antiapoptosis therapeutic tool in Alzheimer's disease

Li,

Jin,

Tan

et al. 2023

CNS Neurosci Ther

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AimsThe aim of this review is to systematically summarize and analyze the noncoding RNAs (ncRNAs), especially microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), in the cell apoptosis among Alzheimer's disease (AD) in recent years to demonstrate their value in the diagnosis and treatment of AD.MethodsWe systematically summarized in vitro and in vivo studies focusing on the ncRNAs in the regulation of cell apoptosis among AD in PubMed, ScienceDirect, and Google Scholar.ResultsWe discover three patterns of ncRNAs (including ‘miRNA‐mRNA’, ‘lncRNA‐miRNA‐mRNA’, and ‘circRNA‐miRNA‐mRNA’) form the ncRNA‐based regulatory networks in regulating cell apoptosis in AD.ConclusionsThis review provides a future diagnosis and treatment strategy for AD patients based on ncRNAs.

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“…Furthermore, a higher level of miR-138 in the SH-SY5Y cells as an AD model contributed to apoptosis by suppressing the PI3K/Akt cascade (Miao et al, 2020). SRY-Box 21 antisense RNA 1 (SOX21-AS1) lncRNA is reported to be upregulated in AD leading to miR-132 down-expression which causes dysregulation in PI3K/Akt pathway, and ultimately results in oxidative stress and apoptosis in Aβ-induced human primary neurons and IMR-32 neuroblastoma cells (F. Gu et al, 2021). Another study reported that miR-132 is a direct target of high-mobility group AT-hook 2 (HM-GA2), a gene transcription regulator, and modulate its expression in reverse order.…”

Section: Ad and The Pten/pi3k/akt Signaling Pathway With Respect To T...mentioning

confidence: 99%

A novel approach to type 3 diabetes mechanism: The interplay between noncoding RNAs and insulin signaling pathway in Alzheimer's disease

Moayedi

Orandi

Ebrahimi

et al. 2022

Journal Cellular Physiology

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Today, growing evidence indicates that patients with type 2 diabetes (T2D) are at a higher risk of developing Alzheimer's disease (AD). Indeed, AD as one of the main causes of dementia in people aged more than 65 years can be aggravated by insulin resistance (IR) and other metabolic risk factors related to T2D which are also linked to the function of the brain. Remarkably, a new term called “type 3 diabetes” has been suggested for those people who are diagnosed with AD while also showing the symptoms of IR and T2D. In this regard, the role of genetic and epigenetic changes associated with AD has been confirmed by many studies. On the other hand, it should be noted that the insulin signaling pathway is highly regulated by various mechanisms, including epigenetic factors. Among these, the role of noncoding RNAs (ncRNAs), including microRNAs and long noncoding RNAs has been comprehensively studied with respect to the pathology of AD and the most well‐known underlying mechanisms. Nevertheless, the number of studies exploring the association between ncRNAs and the downstream targets of the insulin signaling pathway in the development of AD has notably increased in recent years. With this in view, the present study aimed to review the interplay between different ncRNAs and the insulin signaling pathway targets in the pathogenesis of AD to find a new approach in the field of combining biomarkers or therapeutic targets for this disease.

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SRY-Box 21 Antisense RNA 1 Knockdown Diminishes Amyloid Beta25–35-Induced Neuronal Damage by miR-132/PI3K/AKT Pathway

Cited by 10 publications

References 46 publications

Circular RNA (circ)_0129047 upregulates bone morphogenetic protein receptor type 2 expression to inhibit lung adenocarcinoma progression by sponging microRNA (miR)-1206

Circular RNA (circ)_0129047 upregulates bone morphogenetic protein receptor type 2 expression to inhibit lung adenocarcinoma progression by sponging microRNA (miR)-1206

NcRNAs: A synergistically antiapoptosis therapeutic tool in Alzheimer's disease

A novel approach to type 3 diabetes mechanism: The interplay between noncoding RNAs and insulin signaling pathway in Alzheimer's disease

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