SRY: A transcriptional activator of mammalian testis determination

Sekido, Ryohei

doi:10.1016/j.biocel.2009.12.005

Cited by 69 publications

(56 citation statements)

References 36 publications

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“…Sry (sex-determining region Y) together with Nr5a1 (nuclear receptor subfamily 5, group A, member 1) and Wt1 regulate activation of Sox9 expression [49,50]. The expression of Nr5a1, Wt1, and Sox9 were all decreased during mouse testis development and their expression levels were much higher in PN1 than in PN2 and PN3 ( Figure S5).…”

Section: Differentially Expressed Transcription Factorsmentioning

confidence: 99%

“…Sry and its related genes are also similarly down-regulated. Sry is the sex-determining gene on the mammalian Y chromosome and can activate a series of genes that trigger the development of the male genital gland [49]. The expression of Sry is detectable in mice from 10.5 to 12.5 days post coitum until 28 dpn [79].…”

Section: Differential Expression Of Transcription Factorsmentioning

confidence: 99%

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Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

Gong

Pan

Lin

et al. 2012

Sci. China Life Sci.

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Mammalian testis development is a complex and highly sophisticated process. To study the dynamic change of normal testis development at the transcriptional level, we investigated mouse testes at three postnatal ages: 6 days postnatal, 4 weeks old, and 10 weeks old, representing infant (PN1), juvenile (PN2), and adult (PN3) stages, respectively. Using ultra high-throughput RNA sequencing (RNA-seq) technology, we obtained 211 million reads with a length of 35 bp. We identified 18837 genes that were expressed in mouse testes, and found that genes expressed at the highest level were involved in spermatogenesis. The gene expression pattern in PN1 was distinct from that in PN2 and PN3, which indicates that spermatogenesis has commenced in PN2. We analyzed a large number of genes related to spermatogenesis and somatic development of the testis, which play important roles at different developmental stages. We also found that the MAPK, Hedgehog, and Wnt signaling pathways were significantly involved at different developmental stages. These findings further our understanding of the molecular mechanisms that regulate testis development. Our study also demonstrates significant advantages of RNA-seq technology for studying transcriptome during development. next-generation sequencing, transcriptome, mouse testis, development Citation:Gong W, Pan L L, Lin Q, et al. Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing.

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Section: Differentially Expressed Transcription Factorsmentioning

confidence: 99%

Section: Differential Expression Of Transcription Factorsmentioning

confidence: 99%

Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

Gong

Pan

Lin

et al. 2012

Sci. China Life Sci.

View full text Add to dashboard Cite

show abstract

“…whose expression in the embryonic gonadal ridge activates a developmental program leading to testis formation (3). The key initial step is Sry-directed transcriptional activation of autosomal gene Sox9 in pre-Sertoli cells (Fig.…”

mentioning

confidence: 99%

“…Here, we have exploited a DSD-associated mutation in SRY (9) to dissect a key structure-function relationship in the signature domain of an architectural TF. 3 Sry binds and bends specific DNA sites through a conserved protein motif, designated the high mobility group (HMG) box (10). In human SRY, the box is flanked by N-and C-terminal segments lacking conserved or recognizable structural motifs (Fig.…”

mentioning

confidence: 99%

Structure-Function Relationships in Human Testis-determining Factor SRY

Racca

Chen

Maloy

et al. 2014

Journal of Biological Chemistry

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Background: Testis-determining factor SRY provides a model of bent protein⅐DNA complex. Results: Mutation of an invariant Trp perturbs multiple biochemical, cellular, and transcriptional activities. Conclusion: Folding and function of a sequence-specific HMG box require a core "aromatic buttress." Significance: Mutations in SRY causing human sex reversal probe the architecture and evolution of a DNA-bending motif.

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“…There is a proposed model for the SRY/SF1-mediated SOX9 transcriptional regulation in the developing genital ridge (Sekido & Lovell-Badge 2008, Kashimada & Koopman 2010, Sekido 2010). Before E10.5, SF1 may, alone or cooperating with other partners, bind to three putative SF1 consensus-binding sites located within the evolutionarily conserved 1.4 kb TESCO and initiates a very low expression level of Sox9 in the bipotential genital ridge of both sexes (Fig.…”

Section: Genes Implicated In the Regulation Of Sry Expressionmentioning

confidence: 99%

Molecular mechanisms involved in mammalian primary sex determination

She¹,

Yang²

2014

Journal of Molecular Endocrinology

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Sex determination refers to the developmental decision that directs the bipotential genital ridge to develop as a testis or an ovary. Genetic studies on mice and humans have led to crucial advances in understanding the molecular fundamentals of sex determination and the mutually antagonistic signaling pathway. In this review, we summarize the current molecular mechanisms of sex determination by focusing on the known critical sex determining genes and their related signaling pathways in mammalian vertebrates from mice to humans. We also discuss the underlying delicate balance between testis and ovary sex determination pathways, concentrating on the antagonisms between major sex determining genes.

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SRY: A transcriptional activator of mammalian testis determination

Cited by 69 publications

References 36 publications

Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

Structure-Function Relationships in Human Testis-determining Factor SRY

Molecular mechanisms involved in mammalian primary sex determination

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