SRSF1 is crucial for male meiosis through alternative splicing during homologous pairing and synapsis in mice

Sun, Longjie; Chen, Juan; Ye, Rong; Lv, Zheng; Chen, Xuexue; Xie, Xiaomei; Wang, Chaofan; Lv, Pengbo; Yan, Lu; Tian, Shuang; Yao, Xiaohong; Chen, Chen; Cui, Sheng; Liu, Jiali

doi:10.1016/j.scib.2023.04.030

Cited by 7 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concurrently, the results of SRSF1 and PLZF co-staining revealed that SRSF1 was highly expressed in the nuclei of spermatogonia ( Figure 1D ). To further explore the function of SRSF1 in regulating SSC self-renewal and differentiation, crosslinking immunoprecipitation and sequencing (CLIP-seq) was performed in adult mouse testes ( Sun et al, 2023a ). Gene Ontology (GO) enrichment analyses of the SRSF1 peak-containing genes revealed that spermatogenesis-related genes were regulated by SRSF1 ( Figure 2A and Supplementary file 1 ).…”

Section: Resultsmentioning

confidence: 99%

Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

Sun,

Lv,

Chen

et al. 2024

eLife

Self Cite

View full text Add to dashboard Cite

Spermatogonial stem cells (SSCs) are essential for continuous spermatogenesis and male fertility. The underlying mechanisms of alternative splicing (AS) in mouse SSCs are still largely unclear. We demonstrated that SRSF1 is essential for gene expression and splicing in mouse SSCs. Crosslinking immunoprecipitation and sequencing (CLIP-seq) data revealed that spermatogonia-related genes (e.g., Plzf, Id4, Setdb1, Stra8, Tial1/Tiar, Bcas2, Ddx5, Srsf10, Uhrf1, and Bud31) were bound by SRSF1 in the mouse testes. Specific deletion of Srsf1 in mouse germ cells impairs homing and self-renewal of SSCs leading to male infertility. Whole-mount staining data showed the absence of germ cells in the testes of adult cKO mice, which indicates Sertoli cell-only syndrome (SCOS) in cKO mice. The expression of spermatogonia-related genes (Gfra1, Pou5f1, Plzf, Dnd1, Stra8, and Taf4b) was significantly reduced in the testes of conditional knockout (cKO) mice. Moreover, multiomics analysis suggests that SRSF1 directly binds and regulates the expression of Tial1/Tiar via AS to implement SSC homing and self-renewal. In addition, immunoprecipitation mass spectrometry (IP-MS) data showed that SRSF1 interacts with RNA splicing-related proteins (e.g., SRSF10, SART1, RBM15, SRRM2, SF3B6, and SF3A2). Collectively, our data reveal the critical role of SRSF1-mediated AS in SSC homing and self-renewal, which may provide a framework to elucidate the molecular mechanisms of the posttranscriptional network underlying the formation of SSC pools and the establishment of niches.

show abstract

Section: Resultsmentioning

confidence: 99%

Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

Sun,

Lv,

Chen

et al. 2024

eLife

Self Cite

View full text Add to dashboard Cite

show abstract

“…Concurrently, the results of SRSF1 and PLZF co-staining revealed that SRSF1 was highly expressed in the nuclei of spermatogonia (Figure 1D). To further explore the function of SRSF1 in regulating SSC self-renewal and differentiation, CLIP-seq was performed in adult mouse testes (Sun et al, 2023a). GO enrichment analyses of the SRSF1 peak-containing genes revealed that spermatogenesis-related genes were regulated by SRSF1 (Figure 2A and Table S1).…”

Section: Resultsmentioning

confidence: 99%

“…The RNA-seq data were deposited in GEO (https://www.ncbi.nlm.nih.gov/geo/) under accession number GSE227575. The CLIP-seq data were deposited in GEO under accession number GSE227422 (Sun et al ., 2023a).…”

Section: Data Availabilitymentioning

confidence: 99%

“…Serine/arginine-rich splicing factor 1 (SRSF1; previously SF2/ASF) is a widely studied and important splicing factor involved in cancer progression, heart development, and thymus development (Du et al, 2021;Katsuyama et al, 2019;Katsuyama and Moulton, 2021;Liu et al, 2021;Lv et al, 2021;Qi et al, 2021;Xu et al, 2005). Our previous work has shown that SRSF1 deficiency impairs primordial follicle formation during meiotic prophase I and leads to primary ovarian insufficiency (POI) (Sun et al, 2023b).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

Sun

Zheng

Chen

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Spermatogonial stem cells (SSCs) are essential for continuous spermatogenesis and male fertility. The underlying mechanisms of alternative splicing (AS) in mouse SSCs are still largely unclear. We demonstrated that SRSF1 is essential for gene expression and splicing in mouse SSCs. Crosslinking immunoprecipitation and sequencing (CLIP-seq) data revealed that spermatogonia-related genes (e.g.,Plzf,Id4,Setdb1,Stra8,Tial1/Tiar,Bcas2,Ddx5,Srsf10,Uhrf1, andBud31) were bound by SRSF1 in the mouse testes. Specific deletion ofSrsf1in mouse germ cells impairs homing and self-renewal of SSCs leading to male infertility. Whole-mount staining data showed the absence of germ cells in the testes of adult cKO mice, which indicates Sertoli cell-only syndrome (SCOS) in cKO mice. The expression of spermatogonia-related genes (Gfra1,Pou5f1,Plzf,Dnd1,Stra8, andTaf4b) was significantly reduced in the testes of conditional knockout (cKO) mice. Moreover, multiomics analysis suggests that SRSF1 directly binds and regulates the expression ofTial1/Tiarvia AS to implement SSC homing and self-renewal. In addition, immunoprecipitation mass spectrometry (IP-MS) data showed that SRSF1 interacts with RNA splicing-related proteins (e.g., SRSF10, SART1, RBM15, SRRM2, SF3B6, and SF3A2). Collectively, our data reveal the critical role of SRSF1-mediated AS in SSC homing and self-renewal, which may provide a framework to elucidate the molecular mechanisms of the posttranscriptional network underlying the formation of SSC pools and the establishment of niches.

show abstract

New functions and mechanisms of RNA regulation in spermatogenesis and male infertility

PAN,

YIN,

LIU

2024

Sci. Sin.-Vitae

View full text Add to dashboard Cite

SRSF1 is crucial for male meiosis through alternative splicing during homologous pairing and synapsis in mice

Cited by 7 publications

References 14 publications

Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

New functions and mechanisms of RNA regulation in spermatogenesis and male infertility

Contact Info

Product

Resources

About