SRPX2, an independent prognostic marker, promotes cell migration and invasion in hepatocellular carcinoma

Lin, Xiaolan; Chang, Weiping; Wang, Yuan; Tian, Ming; Yu, Zhaoxiang

doi:10.1016/j.biopha.2017.06.075

Cited by 21 publications

(23 citation statements)

References 22 publications

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“…[11] The expression of SRPX2 was also significantly higher in gastric cancer tissue than that in adjacent normal mucosa. In the current study, we showed that the expression of SRPX2 at both mRNA and protein levels was significantly upregulated in human prostate cancer tissues and cell lines.…”

Section: Discussionmentioning

confidence: 93%

“…[8] SRPX2 was reported to regulate endothelial cell migration and tube formation in the vascular endothelial cells. [10][11][12] It is reported that the expression of SRPX2 was significantly increased in human colorectal cancer tissues and cell lines, downregulation of SRPX2 greatly suppressed the proliferation, adhesion, migration, and invasion in human colorectal cancer cells. [10][11][12] It is reported that the expression of SRPX2 was significantly increased in human colorectal cancer tissues and cell lines, downregulation of SRPX2 greatly suppressed the proliferation, adhesion, migration, and invasion in human colorectal cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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Retracted: Knockdown of SRPX2 inhibits the proliferation, migration, and invasion of prostate cancer cells through the PI3K/Akt/mTOR signaling pathway

2018

J Biochem & Molecular Tox

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Sushi repeat‐containing protein X‐linked 2 (SRPX2), a novel chondroitin sulfate proteoglycan, is reported to play a critical role in tumorigenesis. However, the expression and functional role of SRPX2 in prostate cancer have not been defined. Thus, the aim of this study was to investigate the expression and functional role of SRPX2 in human prostate cancer. Our results showed that the expression of SRPX2 was obviously increased in human prostate cancer tissues and cell lines. In addition, knockdown of SRPX2 inhibited the proliferation, migration, and invasion of prostate cancer cells, as well as prevented the epithelial‐mesenchymal transition process in prostate cancer cells. Mechanically, knockdown of SRPX2 efficiently inhibited the activation of PI3K/Akt/mTOR pathway in prostate cancer cells. Taken together, these data demonstrated that knockdown of SRPX2 inhibits the proliferation and metastasis in human prostate cancer cells, partly through the PI3K/Akt/mTOR signaling pathway. Thus, SRPX2 may be a novel therapeutic target for the treatment of prostate cancer.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Retracted: Knockdown of SRPX2 inhibits the proliferation, migration, and invasion of prostate cancer cells through the PI3K/Akt/mTOR signaling pathway

2018

J Biochem & Molecular Tox

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“…Wu et al of cells. 8 Therefore, we further investigated the role and mechanism of SRPX2 in the proliferation and invasion of OS cells. In vitro experiments indicated that SRPX2 knockdown inhibited the viability, invasion and colony formation ability in 143B and U2OS cell lines, while SRPX2 overexpression promoted the viability, invasion and colony formation ability in Mg63 and Saos2 cell lines (Figures 2 and 3).…”

Section: Dovepressmentioning

confidence: 99%

<p>SRPX2 Promotes Cell Proliferation and Invasion in Osteosarcoma Through Regulating Hippo Signaling Pathway</p>

Wu¹,

Wang²,

Chen³

et al. 2020

OTT

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Background/Purpose: Osteosarcoma (OS), a primary bone malignancy, is characterized by a high rate of metastasis. It has been found that Sushi repeat containing protein X-linked 2 (SRPX2) is involved in tumor cell proliferation, adhesion, invasion and migration. The current work aimed to explore the effect of SRPX2 on OS cell invasion and proliferation. Methods: Immunohistochemistry (IHC), Western blotting and reverse transcriptionpolymerase chain reaction (RT-PCR) were used to detect the expression of the associated protein in OS tissues and cell lines. Cell counting kit-8 (CCK8), transwell and colony formation assays were used to determine cell viability, invasion, and proliferation, respectively. The in vivo tumorigenic ability of SRPX2 gene was determined using nude mouse tumorigenesis test. Results: SRPX2 knockdown suppressed the viability, while SRPX2 overexpression increased the invasion and colony formation ability of the cells in vitro. In vivo experiments demonstrated that SRPX2 knockdown inhibited tumor growth and invasion as evidenced by decreased Ki67 and N-cadherin levels, and increased E-cadherin level. Downregulation of SRPX2 increased YAP phosphorylation resulting in reduced nuclear translocation to activate Hippo signaling pathway. The promotion of cell viability, colony-forming ability, and invasion, and the inhibition of CTGF, Cyr61, and Birc5 levels promoted by SRPX2 overexpression were reversed by YAP inhibition. Conclusion: SRPX2 increased cell proliferation and invasion in osteosarcoma by activating Hippo signaling pathway.

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“…Studies of glioblastoma (Tang et al, ), gastric cancer (Tanaka et al, ), liver cancer (Lin, Chang, Wang, Tian, & Yu, ), and colorectal cancer (Liu, Wu, Zhou, & Fan, ; Øster et al, ) showed that SRPX2 is a secreted protein whose expression increases angiogenesis and adhesion of cancer cells. An increased level of tissue SRPX2 is a predictive biomarker for a poor outcome in patients with hepatocellular carcinoma (Lin et al, ). The function of SRPX2 in cancer cells requires interaction with uPAR on the cell surface and engagement of membrane co‐receptors, such as α v β3 integrin and PDGFRβ receptors.…”

Section: Introductionmentioning

confidence: 99%

“…The function of SRPX2 in cancer cells requires interaction with uPAR on the cell surface and engagement of membrane co‐receptors, such as α v β3 integrin and PDGFRβ receptors. This results in intracellular activation of the P12k/Akt, Ras/MAPK, and P‐FAK/Akt pathways, eventually activating MMP2 and MMP9 matrix metalloproteinases (Lin et al, ; Liu, Fan, & Wu, ; Tang et al, ; Yamada et al, ). Expression of SRPX2 in colorectal cancer is regulated by nonCpG island promoter hypomethylation and post‐transcriptionally by miR‐149 (Øster et al, ).…”

Section: Introductionmentioning

confidence: 99%

Sushi repeat‐containing protein X‐linked 2: A novel phylogenetically conserved hypothalamo‐pituitary protein

Anwer

Bolkvadze

Ndode-Ekane

et al. 2018

J of Comparative Neurology

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Sushi repeat-containing protein X-linked 2 (SRPX2) is a novel protein associated with language development, synaptic plasticity, tissue remodeling, and angiogenesis. We investigated the expression and spatial localization of SRPX2 in normal mouse, rat, monkey, and human brain using in situ hybridization and immunohistochemistry. Antibody specificity was determined using in vitro siRNA based silencing of SRPX2. Cell type-specific expression was verified by double-labeling with oxytocin or vasopressin. Western blot was used to detect SRPX2 protein in rat and human plasma and cerebrospinal fluid. Unexpectedly, SRPX2 mRNA expression levels were strikingly higher in the hypothalamus as compared to the cortex. All SRPX2 immunoreactive (ir) neurons were localized in the hypothalamic paraventricular, periventricular, and supraoptic nuclei in mouse, rat, monkey, and human brain. SRPX2 colocalized with vasopressin or oxytocin in paraventricular and supraoptic neurons. Hypothalamic SRPX2-ir positive neurons gave origin to dense projections traveling ventrally and caudally toward the hypophysis. Intense axonal varicosities and terminal arborizations were identified in the rat and human neurohypophysis. SRPX2-ir cells were also found in the adenohypophysis. Light SRPX2-ir projections were observed in the dorsal and ventral raphe, locus coeruleus, and the nucleus of the solitary tract in mouse, rat and monkey. SRPX2 protein was also detected in plasma and CSF. Our data revealed intense phylogenetically conserved expression of SRPX2 protein in distinct hypothalamic nuclei and the hypophysis, suggesting its active role in the hypothalamo-pituitary axis. The presence of SRPX2 protein in the plasma and CSF suggests that some of its functions depend on secretion into body fluids.

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SRPX2, an independent prognostic marker, promotes cell migration and invasion in hepatocellular carcinoma

Cited by 21 publications

References 22 publications

Retracted: Knockdown of SRPX2 inhibits the proliferation, migration, and invasion of prostate cancer cells through the PI3K/Akt/mTOR signaling pathway

Retracted: Knockdown of SRPX2 inhibits the proliferation, migration, and invasion of prostate cancer cells through the PI3K/Akt/mTOR signaling pathway

<p>SRPX2 Promotes Cell Proliferation and Invasion in Osteosarcoma Through Regulating Hippo Signaling Pathway</p>

Sushi repeat‐containing protein X‐linked 2: A novel phylogenetically conserved hypothalamo‐pituitary protein

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