sRNA STnc150 is involved in virulence regulation of Salmonella Typhimurium by targeting fimA mRNA

Li, Jing; Li, Na; Ning, Chengcheng; Guo, Yun; Ji, Chunhui; Zhu, Xiaozhen; Zhang, Xingxing; Meng, Qingling; Shang, Yunxia; Xiao, Chencheng; Xia, Xianzhu; Xue-peng, Cai; Qiao, Jun

doi:10.1093/femsle/fnab124

Cited by 7 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The dual plasmid reporter system (DPRS) was performed according to the described protocol [19]. Briefly, pUT18C- STnc1280 and pMR- gldA-lacZ were constructed according to the protocol of this system.…”

Section: Methodsmentioning

confidence: 99%

STnc1280, a trans-coding sRNA is involved in virulence modulation via targeting gldA mRNA in Salmonella Typhimurium

Ning,

Li,

Wang

et al. 2024

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Introduction. Salmonella Typhimurium (STM) is a food-borne Gram-negative bacterium, which can infect humans and a wide range of livestock and poultry, causing a variety of diseases such as septicaemia, enteritis and abortion. Hypothesis/Gap Statement. We will decipher the impacts of sRNA STnc1280 on STM virulence and provide a theoretical basis to reveal the regulatory role and molecular mechanism of STnc1280. Aim. The main objective of this study was to clarify whether sRNA STnc1280 exerts regulatory roles on STM pathogenicity. Methodology. The STnc1280 gene was amplified and its molecular characteristics were analysed in this study. Then, STnc1280 gene deletion strain (STM-ΔSTnc1280) and the complementary strain (ΔSTnc1280/STnc1280) were constructed by λ-Red homologous recombination method, respectively, to analyse of adhesion and invasive ability and pathogenicity of different strains. Subsequently, the potential target gene regulated by STnc1280 was predicted using target RNA2 software, followed by the verification of the interaction between STnc1280 and target mRNA using the dual plasmid reporter system (DPRS). Furthermore, the mRNA and protein level of target gene was determined using qRT-PCR and Western blot, respectively. Results. The results revealed that the cell adhesion and invasive ability and pathogenicity of STM-ΔSTnc1280 were significantly reduced compared to STM-SL1344 strain, indicating that the deficiency of STnc1280 gene significantly influenced STM pathogenicity. The DPRS results showed that STnc1280 can interact with the mRNA of target gene gldA, thus suppressing the expression of lacZ gene. Furthermore, the level of gldA mRNA was not influenced in STM-ΔSTnc1280, but the expression of GldA protein decreased significantly. Conclusion. Combining the bioinformatic analysis, these findings suggested that STnc1280 may bind to the SD sequence of gldA mRNA, hindering the binding of ribosomes to gldA mRNA, thereby inhibiting the expression of GldA protein to modulate the virulence of STM.

show abstract

Section: Methodsmentioning

confidence: 99%

STnc1280, a trans-coding sRNA is involved in virulence modulation via targeting gldA mRNA in Salmonella Typhimurium

Ning,

Li,

Wang

et al. 2024

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Construction of complementation strains was performed as described previously ( 52 ), with some modifications. The forward primer tonB-compl-F with the NdeI restriction enzyme site and the reverse primer tonB-compl-R containing the XbaI site were used for PCR amplification of tonB .…”

Section: Methodsmentioning

confidence: 99%

Resistance Mechanism and Physiological Effects of Microcin Y in Salmonella enterica subsp. enterica Serovar Typhimurium

et al. 2022

View full text Add to dashboard Cite

show abstract

“…STnc150, another sRNA of S. Typhimurium, downregulates the protein expression of FimA by base-pairing with the 5’-end of fimA mRNA. Deletion of STnc150 enhanced the adhesion ability of S. Typhimurium to macrophages and reduced LD50 in mice [ 48 ].…”

Section: Srnas’ Functions In Salmonella –Host Inte...mentioning

confidence: 99%

Functions of Small Non-Coding RNAs in Salmonella–Host Interactions

Meng

Xia

et al. 2022

Biology

View full text Add to dashboard Cite

Salmonella species infect hosts by entering phagocytic and non-phagocytic cells, causing diverse disease symptoms, such as fever, gastroenteritis, and even death. Therefore, Salmonella has attracted much attention. Many factors are involved in pathogenesis, for example, the capsule, enterotoxins, Salmonella pathogenicity islands (SPIs), and corresponding regulators. These factors are all traditional proteins associated with virulence and regulation. Recently, small non-coding RNAs (sRNAs) have also been reported to function as critical regulators. Salmonella has become a model organism for studying sRNAs. sRNAs regulate gene expression by imperfect base-pairing with targets at the post-transcriptional level. sRNAs are involved in diverse biological processes, such as virulence, substance metabolism, and adaptation to stress environments. Although some studies have reported the crucial roles of sRNAs in regulating host–pathogen interactions, the function of sRNAs in host–Salmonella interactions has rarely been reviewed. Here, we review the functions of sRNAs during the infection of host cells by Salmonella, aiming to deepen our understanding of sRNA functions and the pathogenic mechanism of Salmonella.

show abstract

sRNA STnc150 is involved in virulence regulation of Salmonella Typhimurium by targeting fimA mRNA

Cited by 7 publications

References 25 publications

STnc1280, a trans-coding sRNA is involved in virulence modulation via targeting gldA mRNA in Salmonella Typhimurium

STnc1280, a trans-coding sRNA is involved in virulence modulation via targeting gldA mRNA in Salmonella Typhimurium

Resistance Mechanism and Physiological Effects of Microcin Y in Salmonella enterica subsp. enterica Serovar Typhimurium

Functions of Small Non-Coding RNAs in Salmonella–Host Interactions

Contact Info

Product

Resources

About