SRF is a non-histone methylation target of KDM2B and SET7 in the regulation of myogenesis

Kook, Hyun; Joung, Hosouk; Kang, Joo-Young; Kim, Jiyoung; Kwon, Duk-Hwa; Jeong, Anna; Min, Hyun-Ki; Shin, Sera; Lee, Yun-Gyeong; Kim, Young‐Kook; Seo, Sang-Beom

doi:10.1101/2020.04.17.046342

Cited by 2 publications

(2 citation statements)

References 48 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KDM2B is generally associated with the demethylation of histones H3K4, H3K36, and H3K79, and repression of transcription. Recently, KDM2B was suggested to demethylate the non-histone target serum response factor (SRF), consequently inhibiting skeletal muscle cell differentiation and myogenesis [ 53 ]. On the other hand, KDM2B has also been heavily linked with an increased inflammatory response through epigenetic regulation of interleukin 6 [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Albuquerque

Óvilo

Núñez

et al. 2021

Animals

View full text Add to dashboard Cite

Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed to explore the transcriptomics of the Longissimus lumborum muscle in two local pig breeds with distinct genetic background using next-generation sequencing technology and Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed in the Alentejano (AL) and 15 in the Bísaro (BI) breed. Specific slow type myosin heavy chain components were associated with AL (MYH7) and BI (MYH3) pigs, while an overexpression of MAP3K14 in AL may be associated with their lower loin proportion, induced insulin resistance, and increased inflammatory response via NFkB activation. Overexpression of RUFY1 in AL pigs may explain the higher intramuscular (IMF) content via higher GLUT4 recruitment and consequently higher glucose uptake that can be stored as fat. Several candidate genes for lipid metabolism, excluded in the RNA-seq analysis due to low counts, such as ACLY, ADIPOQ, ELOVL6, LEP and ME1 were identified by qPCR as main gene factors defining the processes that influence meat composition and quality. These results agree with the fatter profile of the AL pig breed and adiponectin resistance can be postulated as responsible for the overexpression of MAP3K14′s coding product NIK, failing to restore insulin sensitivity.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Albuquerque

Óvilo

Núñez

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…SRF acetylation promotes its binding to its serum response element at the muscle-specific gene Acta1 to promote its expression. This regulation is necessary for differentiation and is revered by KDM2B [ 98 ]. These data suggest an indirect function of SETD7 in the regulation of differentiation.…”

Section: Epigenetic Regulation Of Adult Myogenesismentioning

confidence: 99%

Epigenetic regulation of satellite cell fate during skeletal muscle regeneration

et al. 2021

View full text Add to dashboard Cite

In response to muscle injury, muscle stem cells integrate environmental cues in the damaged tissue to mediate regeneration. These environmental cues are tightly regulated to ensure expansion of muscle stem cell population to repair the damaged myofibers while allowing repopulation of the stem cell niche. These changes in muscle stem cell fate result from changes in gene expression that occur in response to cell signaling from the muscle environment.Integration of signals from the muscle environment leads to changes in gene expression through epigenetic mechanisms. Such mechanisms, including post-translational modification of chromatin and nucleosome repositioning, act to make specific gene loci more, or less, accessible to the transcriptional machinery. In youth, the muscle environment is ideally structured to allow for coordinated signaling that mediates efficient regeneration. Both age and disease alter the muscle environment such that the signaling pathways that shape the healthy muscle stem cell epigenome are altered. Altered epigenome reduces the efficiency of cell fate transitions required for muscle repair and contributes to muscle pathology. However, the reversible nature of epigenetic changes holds out potential for restoring cell fate potential to improve muscle repair in myopathies.In this review, we will describe the current knowledge of the mechanisms allowing muscle stem cell fate transitions during regeneration and how it is altered in muscle disease. In addition, we provide some examples of how epigenetics could be harnessed therapeutically to improve regeneration in various muscle pathologies.

show abstract

SRF is a non-histone methylation target of KDM2B and SET7 in the regulation of myogenesis

Cited by 2 publications

References 48 publications

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Epigenetic regulation of satellite cell fate during skeletal muscle regeneration

Contact Info

Product

Resources

About