SR8—The establishment and characterisation of a new ovarian carcinoma cell line and xenograft model

Han, Xin; Papadopoulos, A.; Jones, T. A.; Sheer, Denise; Raju, K. S.

doi:10.1016/0959-8049(95)00549-8

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…The histological examination of the xenografts showed moderately differentiated invasive tumour (s.c. tumour invaded directly into muscle tissue and i.p. tumour into the liver parenchyma) with similar morphology to the original ovarian carcinoma as previously reported 1 . However, inoculation of SR8CT cells into two further groups of eight female nude mice resulted in s.c. xenograft in only one mouse (1/8, 12.5%), with no i.p.…”

Section: Resultssupporting

confidence: 73%

“…The SR8 cell line has been recently established and characterized in this laboratory 1 . This cell line was developed from a patient with advanced epithelial ovarian carcinoma (International Federation of Gynaecology and Obstetrics (FIGO) stage IIIb).…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, we report the investigation of the CT‐induced MET effect with reference to certain measurable cellular parameters using our recently established ovarian carcinoma cell line, SR8 1 …”

Section: Introductionmentioning

confidence: 99%

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Cholera toxin‐induced alteration of the phenotype and behaviour of an ovarian carcinoma cell line, SR8

et al. 1999

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Cholera toxin (CT) has been reported to cause a variety of effects on several different cell types. Recently, CT has been shown to increase the susceptibility of ovarian carcinoma cells to cytotoxicity mediated by a variety of effector cells (natural killer, lymphokine-activated killer cells and tumour-associated lymphocytes derived from ascites of ovarian cancer patients) of both autologous and allogenic background. In the present study, CT demonstrated several effects on a newly established ovarian carcinoma line (SR8)1 when added to the culture medium at a concentration of 12.5 ng/mL for 2 days. Cholera toxin altered SR8 morphology to a uniform polygonal cellular shape, with less cell dispersion than the non-CT treated cells. Cholera toxin prolonged the population doubling time by approximately 10 h. The CT-treated SR8 cells exhibited reduced epidermal growth factor receptor expression (39 versus 50%), and increased carbohydrate antigen 125 expression (45 versus 2%) in both immunocytochemical and quantitative flow cytometric analyses. These changes in morphology and tumour marker expression were reversible when CT was removed from the culture. The CT-treated SR8 cells showed reduced capacity to generate tumours in female nude mice in comparison with non-CT treated cells, which produce both subcutaneous and intraperitoneal xenografts with local invasion in an animal model. Cytogenetic analysis of the cell line SR8 before and during treatment with CT showed no new clonal rearrangements. The possible mechanisms involved and the influence of CT on the biological behaviour of ovarian tumour cells are discussed.

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Section: Resultssupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

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Cholera toxin‐induced alteration of the phenotype and behaviour of an ovarian carcinoma cell line, SR8

et al. 1999

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“…However, accurate in vivo models that mimic the clinical presentation of ovarian cancer are rare. Most of them have been developed in mice [17], which are not relevant models for laparoscopy. In addition, numerous xenograft models are characterized by no-take rates ranging from 10% to 50%, a lack of reproducibility, and poor in vivo passages potential [10,19].…”

Section: Commentsmentioning

confidence: 99%

Description of two new human ovarian carcinoma models in nude rats suitable for laparoscopic experimentation

et al. 2001

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“…Большинство из используемых моделей -это экспериментальные РЯ у мышей [13], которые неудобны для исследования химиоперфузионного лечения. Более того, наиболее часто в литературе встречаются ксенографтные модели, для создания которых линии клеток РЯ человека вводятся иммунодефицитным животным.…”

Section: Discussionunclassified

Ascitic ovarian cancer is an adequate preclinical model of carcinomatosis to study intraperitoneal chemoperfusion treatment

et al. 2019

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To study the mechanisms underlying the effects of intraperitoneal chemoperfusion and to develop the optimal chemoperfusion regimen, an animal model of peritoneal carcinomatosis closely resembles a human model of peritoneal carcinomatosis is required. In our study, the model of peritoneal carcinomatosis in rats with ascitic ovarian cancer was used. material and methods. There were three groups of rats with ascitic ovarian cancer: 1 – the control group (n=15) having no treatment; 2 – rats receiving normothermic intraperitoneal chemoperfusion with cisplatin, 40 mg/kg (n=12); 3 – rats receiving hyperthermic intraperitoneal chemoperfusion with cisplatin, 20 mg/kg (n=14). All animals were euthanized with subsequent autopsy. results. Ascitic ovarian cancer developed in 100 % of the animals injected with the tumor cells. The median overall survival of rats in the control group was 9.5 days. At autopsy, all rats had ascites, and rats surviving 15‒17 days after the tumor cell injection had white tumor nodes measuring 1–3 mm in the greater omentum, intestinal mesentery, parietal and visceral peritoneum. The nodes were histologically verified as metastases of low-differentiated ovarian tumor. In 2 and 5 rats from groups 2 and 3 respectively, metastases in paratracheal lymph nodes and tumor hydrothorax were detected with no evidence of peritoneal carcinomatosis. The median survival of rats in groups 2 and 3 was significantly higher than that in the control group, being 37.5 and 25.5 months, respectively (р=0,256). conclusion. This in vivo study proved that localization of ascitic ovarian tumor, development of the tumor in all animals injected with tumor cells, fast ascites progression and peritoneal carcinomatosis make this ascitic ovarian cancer an adequate preclinical model of peritoneal carcinomatosis to study intraperitoneal chemoperfusion. Further studies are needed to understand the reasons and mechanisms of the tumor hydrothorax development in rats after intraperitoneal chemoperfusion.

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SR8—The establishment and characterisation of a new ovarian carcinoma cell line and xenograft model

Cited by 6 publications

References 20 publications

Cholera toxin‐induced alteration of the phenotype and behaviour of an ovarian carcinoma cell line, SR8

Cholera toxin‐induced alteration of the phenotype and behaviour of an ovarian carcinoma cell line, SR8

Description of two new human ovarian carcinoma models in nude rats suitable for laparoscopic experimentation

Ascitic ovarian cancer is an adequate preclinical model of carcinomatosis to study intraperitoneal chemoperfusion treatment

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