Squid p196, a new member of the myosin-V class of motor proteins, is associated with motile axoplasmic organelles

Cohen, Darien L.

doi:10.1016/s0006-8993(00)03165-6

Cited by 3 publications

(3 citation statements)

References 48 publications

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“…Indeed, actin‐based motors may be required for transported virus to travel from the end of one microtubule to the next, which is hypothesized to occur on actin filaments (Kuznetsov et al ., 1992; Bearer & Reese, 1999). Actin‐based motors are also present in the axon (Bearer et al ., 1993, 1996a,b; Langford et al ., 1994; Tabb et al ., 1998; Molyneaux et al ., 2000; Cohen, 2001; DeGiorgis et al ., 2002), and isolated axoplasmic organelles move on actin‐filaments in vitro (Kuznetsov et al ., 1992; Bearer et al ., 1993, 1996a,b). Both myosin V and myosin II are found on the surfaces of axoplasmic organelles (Kuznetsov et al ., 1992; Bearer et al ., 1993, 1996a,b; Molyneaux & Langford, 1997; Tabb et al ., 1998; Cohen, 2001; DeGiorgis et al ., 2002).…”

Section: Discussionmentioning

confidence: 99%

Fast anterograde transport of Herpes Simplex Virus: Role for the amyloid precursor protein of Alzheimer's disease

2003

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SummaryAnterograde transport of herpes simplex virus (HSV) from its site of synthesis in the neuronal cell body out the neuronal process to the mucosal membrane is crucial for transmission of the virus from one person to another, yet the molecular mechanism is not known. By injecting GFP-labeled HSV into the giant axon of the squid, we reconstitute fast anterograde transport of human HSV and use this as an assay to uncover the underlying molecular mechanism. HSV travels by fast axonal transport at velocities four-fold faster (0.9 µ µ µ µ m/sec average, 1.2 µ µ µ µ m/sec maximal) than that of mitochondria moving in the same axon (0.2 µ µ µ µ m/sec) and ten-fold faster than negatively charged beads (0.08 µ µ µ µ m/sec). Transport of HSV utilizes cellular transport mechanisms because it appears to be driven from inside cellular membranes as revealed by negative stain electron microscopy and by the association of TGN46, a component of the cellular secretory pathway, with GFP-labeled viral particles. Finally, we show that amyloid precursor protein (APP), a putative receptor for the microtubule motor, kinesin, is a major component of viral particles, at least as abundant as any viral encoded protein, while another putative motor receptor, JIP 1/2, is not detected. Conventional kinesin is also associated with viral particles. This work links fast anterograde transport of the common pathogen, HSV, with the neurodegenerative Alzheimer's disease. This novel connection should prompt new ideas for treatment and prevention strategies.

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Section: Discussionmentioning

confidence: 99%

Fast anterograde transport of Herpes Simplex Virus: Role for the amyloid precursor protein of Alzheimer's disease

2003

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“…However, protein components and functions for regulated protein synthesis at the presynaptic terminal are not well characterized and need to be explored. Myosin V, an actin-based molecular motor ubiquitously found in eukaryotes, has been isolated and partially characterized from squid optic lobes and giant axons (Molyneaux et al, 2000;Cohen, 2001;Brown et al, 2004;Tabb et al, 1998). Myosin Vs represent one of the most well characterized classes of unconventional myosins (Langford, 2002;Trybus, 2008;Desnos et al, 2007).…”

mentioning

confidence: 99%

A novel 65 kDa RNA-binding protein in squid presynaptic terminals

et al. 2010

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“…Myosin Va is highly expressed in the human brain 19, as well as in neurons of most other eukaryotes such as rats 25, chickens 26 and squid 27. Taken together, these demonstrations of the ubiquitous localization of myosin Va to neurons and nervous tissue over a broad phylogenetic range underscore the importance of this molecular motor to fundamental neuronal processes.…”

Section: Discussionmentioning

confidence: 83%

Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age

Souza

Dombroski

Machado

et al. 2013

Braz J Med Biol Res

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Myosin Va functions as a processive, actin-based motor molecule highly enriched in the nervous system, which transports and/or tethers organelles, vesicles, and mRNA and protein translation machinery. Mutation of myosin Va leads to Griscelli disease that is associated with severe neurological deficits and a short life span. Despite playing a critical role in development, the expression of myosin Va in the central nervous system throughout the human life span has not been reported. To address this issue, the cerebellar expression of myosin Va from newborns to elderly humans was studied by immunohistochemistry using an affinity-purified anti-myosin Va antibody. Myosin Va was expressed at all ages from the 10th postnatal day to the 98th year of life, in molecular, Purkinje and granular cerebellar layers. Cerebellar myosin Va expression did not differ essentially in localization or intensity from childhood to old age, except during the postnatal developmental period. Structures resembling granules and climbing fibers in Purkinje cells were deeply stained. In dentate neurons, long processes were deeply stained by anti-myosin Va, as were punctate nuclear structures. During the first postnatal year, myosin Va was differentially expressed in the external granular layer (EGL). In the EGL, proliferating prospective granule cells were not stained by anti-myosin Va antibody. In contrast, premigratory granule cells in the EGL stained moderately. Granule cells exhibiting a migratory profile in the molecular layer were also moderately stained. In conclusion, neuronal myosin Va is developmentally regulated, and appears to be required for cerebellar function from early postnatal life to senescence.

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Squid p196, a new member of the myosin-V class of motor proteins, is associated with motile axoplasmic organelles

Cited by 3 publications

References 48 publications

Fast anterograde transport of Herpes Simplex Virus: Role for the amyloid precursor protein of Alzheimer's disease

Fast anterograde transport of Herpes Simplex Virus: Role for the amyloid precursor protein of Alzheimer's disease

A novel 65 kDa RNA-binding protein in squid presynaptic terminals

Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age

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