Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Bartošík, Martin; Paleček, Emil

doi:10.1002/elan.201100079

Cited by 17 publications

(12 citation statements)

References 59 publications

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“…Palecek defined and described the peak II SW , which has high similarity (in accordance with the obtained voltammograms) with our peak B, only in a native double-stranded DNA structure [24,43]. In our samples, only adenine single-stranded oligonucleotides were occurring.…”

Section: Resultssupporting

confidence: 82%

“…From dA 8 there is a probability of the intra-molecular binding of adenines due to the conformational freedom in individual adenine strands and thus the electrochemical signal of peak B is lowered. Peak B, as it has been assumed and confirmed by Palecek’s experiments [ 24 , 43 ], is connected with the formation of binding between various strands. The shape of the obtained curves changed dramatically for the borderline between dA 20 and longer oligonucleotides.…”

Section: Resultssupporting

confidence: 62%

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Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

et al. 2014

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The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected—peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.

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Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 62%

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

et al. 2014

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“…In one group, the changes of electroactivity of nucleotides, such as guanidine, cytosine and adenine have been accommodated upon hybridization. The pioneering work for such type of sensors was done by Paleček and coworkers [19][20][21][22][23][24][25]. The main drawback for this type of DNA biosensors are the quite weak analytical signals.…”

Section: Introductionmentioning

confidence: 98%

Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode

et al. 2012

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The working principle of a genosensor is based on the mechanism of ion‐channel mimetic sensors. The analytical signals generated upon hybridization processes were recorded by a redox active marker [Fe(CN)6]3−/4− present in the sample solution using voltammetric techniques. The developed genosensor was suitable for determination of 20‐mer complementary oligonucleotide sequence, and also of the PCR products containing the complementary 20‐mer sequence in various positions, with detection limits in the 10 pM range. The noncomplementary 20‐mer oligonucleotide sequence as well as the PCR product without complementary region generated very weak response. The good discrimination of the position of the complementary part in the PCR products was observed.

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“…The rst reported observation of electrochemical unwinding and denaturation of DNA at negative potentials was at a mercury drop electrode in 1974. 1,2 In the years since, potential driven changes in DNA conformation at the surfaces of mercury electrodes were studied extensively by the laboratories of Paleček [3][4][5][6][7][8] and Nurnberg. 9-12 A tentative scheme for DNA unwinding at negative electrode potentials was proposed based on electrostatic repulsion of the sugar-phosphate backbone of DNA.…”

Section: Introductionmentioning

confidence: 99%

Denaturation of dsDNA immobilised at a negatively charged gold electrode is not caused by electrostatic repulsion

et al. 2013

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Double-stranded DNA immobilised through a thiol anchor at a gold electrode surface can be unwound and denatured by applying a negative potential. One proposed mechanism for this electrochemical denaturation is that electrostatic field effects are responsible for the destabilisation of the dsDNA through repulsion of the DNA sugar-phosphate backbone away from the electrode surface. Herein, we demonstrate conclusively that electrochemical melting at gold electrodes cannot be explained solely as a simple repulsion mechanism by showing that immobilised DNA denatures at high ionic strengths, where the DNA base-pairs are situated outside of the electrochemical double-layer (and outside the influence of the electric field), and further, that oligomers comprised of the mimic peptide nucleic acid (PNA) can also be denatured at negative potentials, despite the absence of a negatively charged backbone

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Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Cited by 17 publications

References 59 publications

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode

Denaturation of dsDNA immobilised at a negatively charged gold electrode is not caused by electrostatic repulsion

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