Squamous cell carcinoma antigen concentration in fine needle aspiration samples: A new method to detect cervical lymph node metastases of head and neck squamous cell carcinoma

Schaik, Jeroen E. van; Kobold, Anneke Muller; Laan, Bernard F. A. M. van der; Vegt, Bert van der; Hemel, Bettien M. van; Plaat, Boudewijn E. C.

doi:10.1002/hed.25733

Cited by 5 publications

(8 citation statements)

References 12 publications

(17 reference statements)

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“…In our previous retrospective study, we reported a sensitivity of 95.8% and specificity of 74.4% for the detection of SCC lymph node metastases 2 . In the current prospective study, there was a slightly lower sensitivity of 89.4% and a slightly higher specificity of 79.3% for the detection of SCC lymph node metastases.…”

Section: Discussioncontrasting

confidence: 55%

“…In our previous retrospective study, we reported a sensitivity of 95.8% and specificity of 74.4% for the detection of SCC lymph node metastases. 2 In the current prospective study, there was a slightly lower sensitivity of 89.4% and a slightly higher specificity of 79.3% for the detection of SCC lymph node metastases. This in comparison with FNAC, for which we found an 80.0% sensitivity and 100% specificity for the detection of SCC lymph node metastases in only histologyconfirmed samples.…”

Section: Discussioncontrasting

confidence: 54%

“…We recently demonstrated in a retrospective pilot study a 96% sensitivity and 74% specificity for the detection of head and neck SCC lymph node metastases by measuring the SCC antigen (SCC‐Ag) concentration in fine‐needle aspiration (FNA) samples in a population of patients with a suspicious neck mass 2 . This objective measurement of biomarker concentration in FNA samples could serve as an adjunct tool with or without cytologic examination to detect SCC lymph node metastases.…”

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confidence: 99%

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SCC Antigen Concentrations in Fine‐Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis

Schaik

Kobold

Laan

et al. 2022

Otolaryngol.--head neck surg.

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Objective To determine the diagnostic value of measuring squamous cell carcinoma antigen (SCC-Ag) and cancer antigen 15-3 (CA15-3) concentrations in fine-needle aspiration (FNA) samples for the detection of squamous cell carcinoma (SCC) metastases in cervical lymph nodes. Study Design A prospective study with patients consecutively included between November 2018 and May 2021. Setting A tertiary head and neck oncologic center. Methods Out of 138 patients, SCC-Ag concentrations were analyzed in 168 FNA cervical lymph node samples and CA15-3 in 152 samples. Results were compared with FNA cytology (FNAC) or definitive histology to establish sensitivity and specificity rates. Results For the detection of cervical SCC lymph node metastases, SCC-Ag measurement had an 89.4% sensitivity and 79.3% specificity at a cutoff concentration of 0.1 µg/L. Measurement of CA15-3 concentration in addition to SCC-Ag concentration did not lead to improved accuracy for the detection of SCC. In histology-confirmed cases, FNAC had an 80.0% sensitivity and 100% specificity, as opposed to 93.3% and 57.1%, respectively, for SCC-Ag. Conclusion Measurement of SCC-Ag concentration for detection of SCC lymph node metastases has a sensitivity at least comparable to FNAC and could be used as a relatively cheap screening tool in samples with nondiagnostic or indeterminate FNAC results or when multiple lymph nodes are sampled. However, SCC-Ag in FNA samples has a lower specificity than FNAC assessed by pathologists experienced in head and neck oncology. Addition of CA15-3 measurement did not lead to improved accuracy.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussioncontrasting

confidence: 54%

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confidence: 99%

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SCC Antigen Concentrations in Fine‐Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis

Schaik

Kobold

Laan

et al. 2022

Otolaryngol.--head neck surg.

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“…Extensive necrosis is known to accompany not only metastatic malignancy, but also acute inflammation or granulomatous inflammation. In addition, focal necrosis is seen in LNs associated with systemic lupus erythematosus, infectious mononucleosis, and brucellosis [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

“…This trend was consistent with the findings of previous reports. In squamous cell carcinoma, metastatic LNs of the head and neck are often the first signs of malignancy in the inconspicuous organs of this region [ 1 ]. Additionally, cystic changes are often observed in metastatic LNs in squamous cell carcinoma [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Causes of necrotic features in fine-needle aspirates from cervical lymph nodes

Seo

Shin

Lee

et al. 2021

J Pathol Transl Med

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Fine-needle aspiration (FNA) is an excellent, minimally invasive diagnostic technique for evaluating a mass or lesion. In particular, FNA is commonly used for evaluating enlarged lymph nodes (LNs). In many cases, the aspirate can provide conclusive evidence for a diagnosis; sometimes, the cytology reveals only necrosis. Necrosis of LNs is found in various diseases. Malignant neoplasms (including lymphomas and metastatic carcinomas) must first be excluded. In addition, numerous benign conditions, such as tuberculosis (TB) and Kikuchi disease, also cause LN necrosis. When necrosis is identified in an LN FNA cytology sample, pathologists should consider various entities and attempt to find clues that lead to a final diagnosis. However, LN FNA cytology sometimes reveals necrosis alone, and few studies have investigated this situation. The aim of this study was to evaluate the causes of necrosis in FNA of enlarged LNs. MATERIALS AND METHODS We searched the electronic medical record system of the Keimyung University Dongsan Hospital from 2003-2017 to find patients who underwent FNA on cervical LNs. We selected cases with a description of necrotic features on the pathology report. In addition, we also collected the clinical parameters of these cases, including patient age, sex, biopsy findings, other ancillary tests for Mycobacterium tuberculosis (MTB; including acid-fast bacilli staining), and molecular studies carried out using conventional polymerase chain reaction (PCR) or real-time PCR. We classified these cases into five categories: granulomatous inflammation, Kikuchi disease, suppurative inflammation, malignant neoplasm, and necrosis only. When granuloma was included in the microscopic description (e.g., granulomatous inflammation with caseous necrosis, granulomatous inflammation with a necrotic background, or necrosis with a vague granuloma), it was classi

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Evaluation of squamous cell carcinoma antigen 1 expression in oral squamous cell carcinoma (tumor cells and peritumoral T-lymphocytes) and verrucous carcinoma and comparison with normal oral mucosa

et al. 2021

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Evaluation of squamous cell carcinoma antigen 1 expression in oral squamous cell carcinoma (tumor cells and peritumoral T-lymphocytes) and verrucous carcinoma and comparison with normal oral mucosa Background: Squamous cell carcinoma antigen (SCCA) is used as a prognostic marker for recurrence of squamous cell carcinoma in various sites, including head and neck. Studies suggest that its high serum levels are correlated to some clinical features, such as nodal metastasis. However, it is still unknown if high SCCA in patients with SCCA tissue expression in tumor cells are related to peripheral T-lymphocytes. Therefore, we did this study to evaluate SCCA expression in squamous cell carcinoma and verrucous carcinoma and to compare it with normal oral mucosa, also investigating the correlation between serum-based and tissue-based antigen levels.Methodology: In this study, the immunohistochemistry (IHC) technique was used to determine the SCCA1 expression pattern in 81 specimens divided into 3 groups, including oral squamous cell carcinoma, verrucous carcinoma, and normal oral mucosa. Serum-based and tissue-based antigen levels of 20 oral squamous cell carcinoma cases were compared by the western blot assay. SCCA expression was also evaluated and compared in both tumor cells and peripheral T-lymphocytes by the immunofluorescence assay. Results:Our results showed that the SCCA levels in SCC specimens were significantly lower than in verrucous carcinoma and normal and hyperplastic oral mucosa specimens. We found no correlation between the IHC expression of SCCA and serum levels. SCCA was well expressed in both tumor cells and peripheral T-lymphocytes. Conclusion: Decreasing SCCA in SCC specimens suggested that SCC tumor cells may affect more than the serum levels of SCCA in some patients. In addition, expression of SCCA in peripheral T-lymphocytes showed that both tumor cells and T-lymphocytes may cause serum SCCA.

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Squamous cell carcinoma antigen concentration in fine needle aspiration samples: A new method to detect cervical lymph node metastases of head and neck squamous cell carcinoma

Cited by 5 publications

References 12 publications

SCC Antigen Concentrations in Fine‐Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis

SCC Antigen Concentrations in Fine‐Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis

Causes of necrotic features in fine-needle aspirates from cervical lymph nodes

Evaluation of squamous cell carcinoma antigen 1 expression in oral squamous cell carcinoma (tumor cells and peritumoral T-lymphocytes) and verrucous carcinoma and comparison with normal oral mucosa

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