Squalene monooxygenase（SQLE) protects ovarian cancer cells from ferroptosis

Abstract

Background: Ovarian cancer is one of the three major gynecological cancers, which is closely related to squalene monooxygenase (SQLE). We aim to clarify the role of SQLE in ovarian cancer. Methods: The expression of SQLE was detected by qRT-PCR, Western Bolt and immunohistochemistry. The association between SQLE and ferroptosis was demonstrated by TCGA, GTEx database, TMT protein sequencing, qRT-PCR, Western Bolt, immunofluorescence, ROS detection, and lipid peroxide detection. Animal experiments verified the relationship between SQLE and ferroptosis in ovarian cancer. Results: The expression of SQLE increased in ovarian cancer tissues and cell lines. The decreased expression of SQLE caused ferroptosis of ovarian cancer cells, and enhanced the sensitivity of ovarian cancer cells to ferroptosis inducers. Conclusion: Our study shows that SQLE is highly expressed in ovarian cancer tissues and cells, and the high expression of SQLE in ovarian cancer may promote the occurrence and development of ovarian cancer by protecting ovarian cancer cells from ferroptosis, thus enlightening new treatment methods for ovarian cancer.