Sputum zinc concentration and clinical outcome in older asthmatics

Jayaram, Lata; Chunilal, Sanjeev; Pickering, Sandy; Ruffin, Richard E.; Zalewski, Peter D.

doi:10.1111/j.1440-1843.2011.01932.x

Cited by 26 publications

(22 citation statements)

References 42 publications

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“…Plasma Zn constitutes less than 1% of total body Zn [42] and estimations based on blood may therefore not be representative for the level of free or loosely bound levels of Zn in airway tissue. We have recently shown that the levels of Zn ions in induced sputum are decreased in older patients with asthma [43]. Measuring Zn levels in bronchial brushings presents a technical problem in that these brushings are not routinely performed in asthmatic subjects.…”

Section: Discussionmentioning

confidence: 99%

Apical Localization of Zinc Transporter ZnT4 in Human Airway Epithelial Cells and Its Loss in a Murine Model of Allergic Airway Inflammation

et al. 2011

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The apical cytoplasm of airway epithelium (AE) contains abundant labile zinc (Zn) ions that are involved in the protection of AE from oxidants and inhaled noxious substances. A major question is how dietary Zn traffics to this compartment. In rat airways, in vivo selenite autometallographic (Se-AMG)-electron microscopy revealed labile Zn-selenium nanocrystals in structures resembling secretory vesicles in the apical cytoplasm. This observation was consistent with the starry-sky Zinquin fluorescence staining of labile Zn ions confined to the same region. The vesicular Zn transporter ZnT4 was likewise prominent in both the apical and basal parts of the epithelium both in rodent and human AE, although the apical pools were more obvious. Expression of ZnT4 mRNA was unaffected by changes in the extracellular Zn concentration. However, levels increased 3-fold during growth of cells in air liquid interface cultures and decreased sharply in the presence of retinoic acid. When comparing nasal versus bronchial human AE cells, there were significant positive correlations between levels of ZnT4 from the same subject, suggesting that nasal brushings may allow monitoring of airway Zn transporter expression. Finally, there were marked losses of both basally-located ZnT4 protein and labile Zn in the bronchial epithelium of mice with allergic airway inflammation. This study is the first to describe co-localization of zinc vesicles with the specific zinc transporter ZnT4 in airway epithelium and loss of ZnT4 protein in inflamed airways. Direct evidence that ZnT4 regulates Zn levels in the epithelium still needs to be provided. We speculate that ZnT4 is an important regulator of zinc ion accumulation in secretory apical vesicles and that the loss of labile Zn and ZnT4 in airway inflammation contributes to AE vulnerability in diseases such as asthma.

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Section: Discussionmentioning

confidence: 99%

Apical Localization of Zinc Transporter ZnT4 in Human Airway Epithelial Cells and Its Loss in a Murine Model of Allergic Airway Inflammation

et al. 2011

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“…8,11 Several studies observed the association between low zinc statuses either in serum, hair or sputum and asthma prevalence. 12-17 A small number of studies have been conducted to evaluate the efficacy of zinc supplementation for improvement lung functions or clinical symptoms of asthma during non-exacerbation; however, the results were not consistent. 18-20 Up to present, there is no report of zinc supplementation improving the clinical severity during asthmatic exacerbation.…”

Section: Introductionmentioning

confidence: 99%

Zinc Supplementation in Children with Asthma Exacerbation

Rerksuppaphol

2016

Pediatric Reports

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Zinc deficiency has demonstrated an association with the risk of asthma. This study aimed to evaluate the efficacy of zinc supplementation in reducing the severity of childhood asthma exacerbation. A number of 42 children with asthma exacerbation admitted to the hospital were randomized to receive either zinc bis-glycinate (30 mg elemental zinc/day) or a placebo in adjuvant to the standard treatment. The pediatric respiratory assessment measure (PRAM) was used to measure the asthma severity. The primary outcome was a change in asthma severity from the baseline to the end of study. The study found that PRAM score in the zinc group showed a more rapid decrease compared to the control group at the 24-hour (2.2±1.3 vs. 1.2±1.3; P = 0.015) and 48-hour (3.4±2.0 vs. 2.2±1.8; P = 0.042) intervals. At admission, overall mean serum zinc level was 63.8 mg/dL and 57.1% of children had zinc deficiency with no difference in prevalence between groups. PRAM scores did not differ between children with low and normal zinc status. In conclusion, zinc supplementation as the adjuvant therapy to the standard treatment during asthma exacerbation resulted in rapid lessening of severity.

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“…17 Başka bir çalışmada ise 114 yaşlı astımlıda astım şiddeti ve FEV1 değeri labil balgam çinko düzeyi ile korele bulunmuştur. 42 El Khoyl ve ark. tarafından yapılan 22 astımlı hasta ve 20 kontrol grubunun karşılaştırıldığı bir çalışmada saç ve serum çinko düzeyleri düşük bulunmuştur.…”

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Role of Zinc Deficiency in Allergic Diseases

Özdemir¹,

Tabanlı²

2016

Sakarya Med J

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ÖzÇinko organizma için esansiyel olup vücutta en bol bulunan ikinci eser elementtir. Hücrelerin yapısal ve fonksiyonel bütünlüğü için kritik bir role sahiptir. Ciddi çinko eksikliğinde büyüme geriliği, hipogonadizm, cilt hastalıkları, tat ve koku bozuklukları, bağışıklık sisteminde bozukluk, nöronal ve sensoral bozukluklar gibi birçok patolojiler ortaya çıkabilir. Günümüzde çinko eksikliği tüm dünyada yaygın bir sorun olarak bilinmektedir. Bu derlemede çinko eksikliğinin insan sağlığı üzerindeki ciddi etkileri ve alerjik hastalıklar ile ilişkisi anlatılmaktadır. Abstract Zinc is an essential for the organism, is the second most abundant trace element in the body. It is critical for the structural and functional integrity of cells. Many pathologies may occur in

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Sputum zinc concentration and clinical outcome in older asthmatics

Abstract: These findings suggest that sputum [Zn] reflect clinical outcomes and underlying AI, suggesting a potential role for zinc as a biomarker in asthma.

Cited by 26 publications

References 42 publications

Apical Localization of Zinc Transporter ZnT4 in Human Airway Epithelial Cells and Its Loss in a Murine Model of Allergic Airway Inflammation

Apical Localization of Zinc Transporter ZnT4 in Human Airway Epithelial Cells and Its Loss in a Murine Model of Allergic Airway Inflammation

Zinc Supplementation in Children with Asthma Exacerbation

Role of Zinc Deficiency in Allergic Diseases

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