SPS, a sulfated galactoglucan of Laetiporus sulphureus, exhibited anti-inflammatory activities

Lu, Mei-Kuang; Jen, Chia-I; Chao, Chi-Hsein; Hsu, Yu-Chi; Ng, Lean‐Teik

doi:10.1016/j.ijbiomac.2022.11.237

Cited by 12 publications

(4 citation statements)

References 44 publications

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“…The antioxidant activity of plant-derived polysaccharides is influenced by factors such as uronic acid content, monosaccharide composition, types of glycosidic linkage, and conformation. 46 . In this context, the reducing property is often linked to the presence of reductones, contributing to antioxidant ability by breaking free radical chains.…”

Section: Discussionmentioning

confidence: 99%

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of heteroglycans from Hibiscus sabdariffa calyx

Kanemoto Ngomoyogoli,

Ella,

Kanemoto

et al. 2024

J. Drug Delivery Ther.

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The hot-water extraction method has been employed to enhance the diffusion rate of heteroglycans, thereby augmenting the extraction efficiency. This study investigates the antioxidant activities and immunological properties of water-soluble polysaccharides derived from the Hibiscus sabdariffa calyx (HWPF and LWPF). These polysaccharides were isolated through two distinct extraction methods involving hot water followed by ethanol precipitation. The physicochemical characterization was conducted through a series of experiments encompassing monosaccharide composition, ultraviolet-visible spectrum analysis, and Fourier-transform infrared (FT-IR) spectroscopy. The evaluation of antioxidative properties employed various techniques, including DPPH and ABTS radical scavenging activities, Ferric Reducing Antioxidant Power, ferrous chelating power, total reducing power, and protection against DNA damage. The assessment of anti-inflammatory potential involved the inhibition of hemolytic activity induced by a hypotonic solution and heat. Furthermore, the impact of the polysaccharides on the production of pro-inflammatory cytokines was analyzed through Luminex assay and RTPCR assay in PBMCs and LPS-stimulated RAW 264.7 cells, respectively. The findings revealed that heteroglycans (LWPF and HWPF) exhibit a content of Ara-Man-Rha-GluA-GalA (41.2:17.2:15.7:9.2:8.8) and Ara-Glu-Rha-Xyl-GluA-Man (31.5:23.8:12.16:10.4:7:6) with molecular weights of approximately 4.5 KDa and 41.3 KDa, respectively. HWPF demonstrated robust metal chelating and scavenging activities. Additionally, each fraction exhibited substantial protection of DNA against damage induced by H2O2. LWPF was observed to significantly down-regulate the expression of pro-inflammatory cytokines IL-6 and COX-2 at both transcriptional and translational levels, along with inhibiting NO production. In summary, these results indicate that the choice of extraction methods influences both the structure and biological activities of heteroglycans, providing valuable insights into the structure-activity relationship. Keywords: Hibiscus sabdariffa, heteroglycans, hot water extraction, physicochemical properties, Antioxidant activity, Pro-inflammatory cytokines.

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Section: Discussionmentioning

confidence: 99%

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of heteroglycans from Hibiscus sabdariffa calyx

Kanemoto Ngomoyogoli,

Ella,

Kanemoto

et al. 2024

J. Drug Delivery Ther.

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“…The structural attributes of polysaccharides generated from natural sources are significantly correlated with their biological activity [49]. A galactoglucan of Laetiporus sulphureus inhibited secretion of inflammatory factor in LPSinduced RAW 264.7 macrophages with promising anti-inflammatory activity [50]. Likewise, both Pellinus polysaccharide, primarily composed of lactose, and Poria cocos polysaccharide demonstrate efficacy against inflammatory disorders [51,52].…”

Section: Discussionmentioning

confidence: 99%

Chroogomphus rutilus Regulates Bone Metabolism to Prevent Periodontal Bone Loss during Orthodontic Tooth Movement in Osteoporotic Rats

Zhou,

Zhu,

Jin

et al. 2023

Nutrients

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Osteoporosis (OP) leads to the acceleration of tooth movement and aggravation of periodontal bone loss during orthodontic treatment. Chroogomphus rutilus (CR) is abundant in nutrients and demonstrates remarkable antioxidant and anti-inflammatory properties. In the present study, the components of CR, including 35.00% total sugar, 0.69% reducing sugar, 14.40% crude protein, 7.30% total ash, 6.10% crude fat, 0.51% total flavonoids, 1.94% total triterpenoids, 0.32% total sterol, 1.30% total saponins, 1.69% total alkaloids, and 1.02% total phenol, were first systematically examined, followed by an investigation into its regulatory effects on bone metabolism in order to mitigate bone loss during orthodontic tooth movement in osteoporotic rats. The results of the imaging tests revealed that CR treatment reduced periodontal bone loss and normalized tooth movement in the OP. In conjunction with analyses of intestinal flora and metabolomics, CR enhances the prevalence of anti-inflammatory genera while reducing the production of inflammatory metabolites. Meanwhile, CR reduced the levels of periodontal inflammatory factors, including TNF-α, IL-1β, and IL-6, by activating Wnt/β-catenin signaling, and promoted periodontal bone formation. These findings imply that CR is a potent supplementary therapy for controlling periodontal bone remodeling in patients with OP undergoing orthodontic treatment.

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“…But the peak of ASPPs had a bit tailing peak, which might be caused by partial hydrolysis of polysaccharide. [22] The Mw has been confirmed as an important parameter to affect the biological activities of polysaccharides, so the Mw of ASPs were analyzed. The standard curve was established with logMw and retention time of the dextran standard.…”

Section: Homogeneity and Mw Analysismentioning

confidence: 99%

Phosphorylation Modification, Structural Characterization, Antioxidant and DNA Protection Capacities of Polysaccharides from Asarum Sieboldii Miq

Yang,

Wang,

Zhang

et al. 2024

Chemistry & Biodiversity

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Polysaccharide from Asarum sieboldii Miq (ASP) was extracted and five phosphorylation polysaccharides with different degree of substitution were obtained, namely ASPP1, ASPP2, ASPP3, ASPP4, and ASPP5 (ASPPs). The physical and chemical structure and biological activities were studied. The results suggested that the carbohydrate and protein content were reduced while uronic acid was increased after phosphorylation modification. The molecular weight of ASPPs was significantly lower than that of ASP. ASPPs were acidic heteropolysaccharides mainly composed of galacturonic acid, galactose, glucose, fructose, and arabinose. The UV‐vis spectrum indicated that the polysaccharides did not contain nucleic acid or protein after modification. The Fourier transform infrared spectrum demonstrated that ASPPs contained characteristic absorption peaks of P=O and P‐O‐C near 1270 and 980 cm‐1. ASPPs presented a triple helix conformation, but it was not presented in ASP. The scanning electron microscopy analysis showed that the surface topography and particle structure of ASP were different after modification. Compared with ASP, ASPPs enhanced the activity to scavenge DPPH and ABTS free radicals and possessed more protective ability to DNA oxidation caused by OH·, GS·, and AAPH free radicals. These results suggest that chemical modification is beneficial for the exploitation and utilization of natural polysaccharides.

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SPS, a sulfated galactoglucan of Laetiporus sulphureus, exhibited anti-inflammatory activities

Cited by 12 publications

References 44 publications

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of heteroglycans from Hibiscus sabdariffa calyx

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of heteroglycans from Hibiscus sabdariffa calyx

Chroogomphus rutilus Regulates Bone Metabolism to Prevent Periodontal Bone Loss during Orthodontic Tooth Movement in Osteoporotic Rats

Phosphorylation Modification, Structural Characterization, Antioxidant and DNA Protection Capacities of Polysaccharides from Asarum Sieboldii Miq

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