Sprühreagens zum Nachweis von Aflatoxinen

Mücke, W.; Kiermeier, Friedrich

doi:10.1007/bf01810716

Cited by 10 publications

(4 citation statements)

References 2 publications

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“…a) internal standard of aflatoxins, added on a chromatoplate b) spraying chromatograms with 25% sulphuric acid [11] c) spraying chromatograms with di-o-anisidine [7] d) formation of water adducts and acetate adducts [21] e) measurement of growth inhibition zone of Bacillus megateviurn on PETRI dishes [3]…”

Section: Results Of Tests Which Confirm the Jwesence Of Aflatoxins Inmentioning

confidence: 99%

“…The presence of some fluorescent impurities in crops grains extracted in the same way like aflatoxins with similar fluorescent properties make it necessary to perform additional tests in order to confirm the presence of aflatoxins in the extracts. a) internal standard of aflatoxins, added on a chromatoplate b) spraying chromatograms with 25% sulphuric acid [11] c) spraying chromatograms with di-o-anisidine [7] d) formation of water adducts and acetate adducts [21] e) measurement of growth inhibition zone of Bacillus megateviurn on PETRI dishes [3]…”

Section: Results Of Tests Which Confirm the Jwesence Of Aflatoxins Inmentioning

confidence: 99%

“…Change of fluorescence colour was performed according to PONS et al [11] and the colour test with di-o-anisidine recommended by MUCKE et al [7] was used.…”

Section: Water Adducts Were Prepared As Followsmentioning

confidence: 99%

“…Mean recovery of aflatoxin B, standard after column was about 96%. and oats (7,8). Extracts from wheat (2).…”

Section: Purification Of Extractmentioning

confidence: 99%

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Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B₁, B₂, G₁ and G₂ contents in Grain crops¹

Janicki

Szebiotko

Kokorniak

et al. 1974

Nahrung

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The simple method for determination of small amounts of aflatoxins (about 5 -10 yg/kg) was described. The method was adopted for wheat, barley, rye and oats. Difficulties of aflatoxins determination in cereals are discussed, mainly observed during purification of extracts and resolution by TLC. Different tests were compared for confirmation of aflatoxins in cereals.Results of cereal crops control for contamination with aflatoxins are presented.The differences between results obtained in collaborative studies on the determination of aflatoxin in corn are very considerable, especially for samples of corn with content of toxins lower than IOO Fg per kg [IS, 211. Standards admitted by WHO, on contents of aflatoxins of about 10 yg per kg request the elaboration of considerably more sensitive methods. The detection as well as the precise determination of such small amounts of aflatoxins in a sample creates additionally analytical difficulties. In the greater part of products contaminated by aflatoxins there is a considerable amount of impurities which make impossible their determination without a previous thorough removal of the impurities from the extract [I, 4, 6 , 8-10, 12, 13, 15, 16, 20, 221.As it appears from literature information and from our own experience the reasons for the diversified results in the quantitative determination of aflatoxins by chromatographic methods can be listed as follows:I. Distribution of aflatoxins material is often not uniform. Such a distribution causes considerable differences in the results of parallel average samples. 2. Losses during the extraction caused by the use of improper solvents for instance in methanol by decomposition under influence of light, etc. 3. Losses during the purification on chromatographic columns. 4. Improper resolution on thin-layer chromatograms.5. Decomposition on chromatograms especially under the influence of light.6. Masking the fluorescence of aflatoxins spots by impurities which fluoresce or quench the fluorescence, especially in grain extracts.Our investigations have confirmed these observation;, because in each stage we have stated losses of aflatoxins. For this reason we have controlled on each stage (extraction, purification, TLC resolution and fluorodensitometry) in order to find out the causes of the losses.We also investigated the possibility of a most simple method elaboration for aflatoxin control in grain crops.

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Section: Results Of Tests Which Confirm the Jwesence Of Aflatoxins Inmentioning

confidence: 99%

Section: Results Of Tests Which Confirm the Jwesence Of Aflatoxins Inmentioning

confidence: 99%

“…Change of fluorescence colour was performed according to PONS et al [11] and the colour test with di-o-anisidine recommended by MUCKE et al [7] was used.…”

Section: Water Adducts Were Prepared As Followsmentioning

confidence: 99%

“…Mean recovery of aflatoxin B, standard after column was about 96%. and oats (7,8). Extracts from wheat (2).…”

Section: Purification Of Extractmentioning

confidence: 99%

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Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B₁, B₂, G₁ and G₂ contents in Grain crops¹

Janicki

Szebiotko

Kokorniak

et al. 1974

Nahrung

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Aflatoxins in Foods and Feedstuffs. Part 3. Fluorodensitometric method for determination of aflatoxins M₁ and M₂ in presence of aflatoxins B₁, B₂, G₁ and G₂¹

Szebiotko

Chełkowski

Kokorniak

et al. 1974

Nahrung

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The method of aflotoxins M, and M, determination in presence of aflatoxins B,, B,, G, and G, is presented. The fluorescence properties of aflatoxin M, and M, are discribed. A simple method of aflatoxin M extraction is proposed. Results of different tests for confirmation of aflatoxins M are discussed. During control of powdered milk from three factories aflatoxins M were not detected in final products. As aflatoxins M, and M, usually accompany aflatoxins B,, B,, G, and G, as well as their other metabolites present mainly in milk and milk products it is reasonable to determine in the samples affected by mould simultaneously all six mentioned toxins [I, 3, 8, 9, 101. To achieve this simultaneously the determination of all six toxins has been carried out in order to choose the most convenient method for extraction, purification and chromatographic separation. Further more there have been examined the fluorescent properties of the aflatoxins MI and M, in solutions and on chromatograms. Material and MethodsA quantitative standard of the aflatoxin M, having a concentration of 10,14 pg/ml and moreover a standard of the aflatoxin M, having a concentration 6,o Fg/ml. These standards have been received from the Northern Marketing and Nutrition Research Division, Fermentation Laboratory Peoria, Illinois. A qualitative standard of the aflatoxin M,, from the Central Veterinary Laboratory, New Haw Weybridge Surrey, England. Echtblausalz B (fast blue salt B for chromatography) from the firm Merck (di-o-anisidine, tetrazolium chloride).

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Terminal and subterminal oxidation of n-alkanes by molds

Pelz

Rehm

1973

Archiv. Mikrobiol.

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Sprühreagens zum Nachweis von Aflatoxinen

Cited by 10 publications

References 2 publications

Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B₁, B₂, G₁ and G₂ contents in Grain crops¹

Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B₁, B₂, G₁ and G₂ contents in Grain crops¹

Aflatoxins in Foods and Feedstuffs. Part 3. Fluorodensitometric method for determination of aflatoxins M₁ and M₂ in presence of aflatoxins B₁, B₂, G₁ and G₂¹

Terminal and subterminal oxidation of n-alkanes by molds

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Sprühreagens zum Nachweis von Aflatoxinen

Cited by 10 publications

References 2 publications

Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B1, B2, G1 and G2 contents in Grain crops1

Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B1, B2, G1 and G2 contents in Grain crops1

Aflatoxins in Foods and Feedstuffs. Part 3. Fluorodensitometric method for determination of aflatoxins M1 and M2 in presence of aflatoxins B1, B2, G1 and G21

Terminal and subterminal oxidation of n-alkanes by molds

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Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B₁, B₂, G₁ and G₂ contents in Grain crops¹

Aflatoxins in Foods and Feedstuffs. Part 2. Determination of the aflatoxin B₁, B₂, G₁ and G₂ contents in Grain crops¹

Aflatoxins in Foods and Feedstuffs. Part 3. Fluorodensitometric method for determination of aflatoxins M₁ and M₂ in presence of aflatoxins B₁, B₂, G₁ and G₂¹