Spreading of Tissue Spheroids on an Electrospun Polyurethane Matrix

Koudan, Elizaveta V.; Буланова, Елена А.; Pereira, Frederico D. A. S.; Parfenov, Vladislav A.; Kasyanov, Vladimir; Hesuani, U. J.

doi:10.1007/s10527-016-9575-5

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…The surface could be sensed through the 3D microsphere, and the cell on the periphery could be receiving the information cues to begin to spread and colonize the membrane following the random orientation of the PLA fiber. These cell behaviors are in agreement with previous studies reporting that the microenvironment, the physical signals mediated by the fibers mimicking the ECM, and the surface energy regulating the adhesive properties of the spreading and migration of cells could be utilized as a quantitative indicator of tissue biocompatibility [53][54][55].…”

Section: Discussionsupporting

confidence: 91%

In Vitro Bone Differentiation of 3D Microsphere from Dental Pulp-Mesenchymal Stem Cells

et al. 2023

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Bone defects lead to the structural loss of normal architecture, and those in the field of bone tissue engineering are searching for new alternatives to aid bone regeneration. Dental pulp-mesenchymal stem cells (DP-MSC) could provide a promising alternative to repair bone defects, principally due to their multipotency and capacity to fabricate three-dimensional (3D) spheroids. The present study aimed to characterize the 3D DP-MSC microsphere and the osteogenic differentiation capacity potential cultured by a magnetic levitation system. To achieve this, the 3D DP-MSC microsphere was grown for 7, 14, and 21 days in an osteoinductive medium and compared to 3D human fetal osteoblast (hFOB) microspheres by examining the morphology, proliferation, osteogenesis, and colonization onto PLA fiber spun membrane. Our results showed good cell viability for both 3D microspheres with an average diameter of 350 μm. The osteogenesis examination of the 3D DP-MSC microsphere revealed the lineage commitment, such as the hFOB microsphere, as evidenced by ALP activity, the calcium content, and the expression of osteoblastic markers. Finally, the evaluation of the surface colonization exhibited similar patterns of cell-spreading over the fibrillar membrane. Our study demonstrated the feasibility of forming a 3D DP-MSC microsphere structure and the cell-behavior response as a strategy for the applications of bone tissue guiding.

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Section: Discussionsupporting

confidence: 91%

In Vitro Bone Differentiation of 3D Microsphere from Dental Pulp-Mesenchymal Stem Cells

et al. 2023

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“…As a follow-up to our ongoing studies on the systematic characterization of different types of TS, 24,25 we elaborate on CS in this work. For that purpose, we evaluated their biomechanical properties using a tensiometry method combined with routine histological analysis of structural determinants to predict the most important properties of TS, spreading on adhesive substrates and fusion.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Matrix Determines Biomechanical Properties of Chondrospheres during Their Maturation In Vitro

et al. 2018

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Objective Chondrospheres represent a variant of tissue spheroids biofabricated from chondrocytes. They are already being used in clinical trials for cartilage repair; however, their biomechanical properties have not been systematically investigated yet. The aim of our study was to characterize chondrospheres in long-term in vitro culture conditions for morphometric changes, biomechanical integrity, and their fusion and spreading kinetics. Results It has been demonstrated that the increase in chondrospheres secant modulus of elasticity is strongly associated with the synthesis and accumulation of extracellular matrix. Additionally, significant interplay has been found between biomechanical properties of tissue spheroids and their fusion kinetics in contrast to their spreading kinetics. Conclusions Extracellular matrix is one of the main structural determinants of chondrospheres biomechanical properties during chondrogenic maturation in vitro. The estimation of tissue spheroids' physical behavior in vitro prior to operative treatment can be used to predict and potentially control fusogenic self-assembly process after implantation in vivo.

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