Spread of the autonomous transposable element hobo in the genome of Drosophila melanogaster.

Galindo, Máximo Ibo; Ladevèze, Véronique; Lemeunier, Françoise; Kalmes, Robert; Périquet, Georges; Pascual, Luis Alberto Malvárez

doi:10.1093/oxfordjournals.molbev.a040251

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Finally, our data reveal a gradation of degeneration in the TEs and other REs with the ages of the gene duplicates that the repeats flank ( Figure 5 ). This gradation is consistent with observed degeneration rate of functionless elements in Drosophila [ 30 ], as well as any potential internal deletions that could be part of a self-regulation system as seen in D. melanogaster TEs [ 45 ].…”

Section: Resultssupporting

confidence: 85%

Repetitive Element-Mediated Recombination as a Mechanism for New Gene Origination in Drosophila

Yang

Arguello

et al. 2008

PLoS Genet

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Previous studies of repetitive elements (REs) have implicated a mechanistic role in generating new chimerical genes. Such examples are consistent with the classic model for exon shuffling, which relies on non-homologous recombination. However, recent data for chromosomal aberrations in model organisms suggest that ectopic homology-dependent recombination may also be important. Lack of a dataset comprising experimentally verified young duplicates has hampered an effective examination of these models as well as an investigation of sequence features that mediate the rearrangements. Here we use ∼7,000 cDNA probes (∼112,000 primary images) to screen eight species within the Drosophila melanogaster subgroup and identify 17 duplicates that were generated through ectopic recombination within the last 12 mys. Most of these are functional and have evolved divergent expression patterns and novel chimeric structures. Examination of their flanking sequences revealed an excess of repetitive sequences, with the majority belonging to the transposable element DNAREP1 family, associated with the new genes. Our dataset strongly suggests an important role for REs in the generation of chimeric genes within these species.

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Section: Resultssupporting

confidence: 85%

Repetitive Element-Mediated Recombination as a Mechanism for New Gene Origination in Drosophila

Yang

Arguello

et al. 2008

PLoS Genet

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“…The strains were obtained from the Bloomington Drosophila Stock Center and characterized using molecular (PCR analysis) and genetic (instability of the mini-white locus) methods, which made it possible to determine the content of full-length hobo copies in their genomes (Galindo et al, 1995;Bazin and Higuet, 1996;Zakharenko et al, 2006;Yushkova and Zainullin, 2014).…”

Section: Fly Strainsmentioning

confidence: 99%

“…OR S (genotype: C 1 DX, y 1 f 1 ;OR) is a subline of the H-cytotype with attached X-chromosomes. It is derived from the wild-type Oregon-R (OR) strain containing full functional copies of the hobo element in the genotype (Galindo et al, 1995). It was created by crossing three females of the C 1 DX, y 1 f 1 /Y strain with three OR males followed by backcrossing their offspring (females) with OR males, for 8 generations (Margulies, 1990).…”

Section: Strains With Functional Hobo Transposonsmentioning

confidence: 99%

“…HiR S (genotype: C 1 DX, y 1 f 1 ; HiR) is a subline of the E-cytotype that does not have hobo transposons. It was obtained according to the same crossing scheme as the OR S subline (from the wild-type Hikone-R strain which does not contain hobo elements (Galindo et al, 1995). We used it in crosses as a reactive (sensitive to hobo transposition) E-cytotype strain that is not subject to mutability in the mini-white locus.…”

Section: Strains Without Hobo Transposonsmentioning

confidence: 99%

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Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Yushkova

2020

Front. Genet.

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The study of the genetic basis of the manifestation of radiation-induced effects and their transgenerational inheritance makes it possible to identify the mechanisms of adaptation and possible effective strategies for the survival of organisms in response to chronic radioactive stress. One persistent hypothesis is that the activation of certain genes involved in cellular defense is a specific response of the cell to irradiation. There is also data indicating the important role of transposable elements in the formation of radiosensitivity/radioresistance of biological systems. In this work, we studied the interaction of the systems of hobo transposon activity and DNA repair in the cell under conditions of chronic low-dose irradiation and its participation in the inheritance of radiation-induced transgenerational instability in Drosophila. Our results showed a significant increase of sterility and locus-specific mutability, a decrease of survival, fertility and genome stability (an increase the frequency of dominant lethal mutations and DNA damage) in non-irradiated F1/F2 offspring of irradiated parents with dysfunction of the mus304 gene which is responsible for excision and post-replicative recombination repair and repair of double-stranded DNA breaks. The combined action of dysfunction of the mus309 gene and transpositional activity of hobo elements also led to the transgenerational effects of irradiation but only in the F1 offspring. Dysfunction of the genes of other DNA repair systems (mus101 and mus210) showed no visible effects inherited from irradiated parents subjected to hobo transpositions. The mei-41 gene showed specificity in this type of interaction, which consists in its higher efficiency in sensing events induced by transpositional activity rather than irradiation.

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“…Class 2 TEs often accumulate internal deletions over time that render the elements inactive and so unable to cause further harm to the host organism [24]. The presence of intact forms of Herves and other hAT TEs, such as Hermes , indicate that at least some hAT elements appear, for reasons unknown, less prone to accumulating internal deletions however the significance of this in absence of information concerning MITEs (Miniature Inverted Terminal Elements) generated from them remains unknown [23,25,26]. …”

Section: Introductionmentioning

confidence: 99%

DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae

et al. 2011

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BackgroundDetermining the mechanisms by which transposable elements move within a genome increases our understanding of how they can shape genome evolution. Class 2 transposable elements transpose via a 'cut-and-paste' mechanism mediated by a transposase that binds to sites at or near the ends of the transposon. Herves is a member of the hAT superfamily of class 2 transposons and was isolated from Anopheles gambiae, a medically important mosquito species that is the major vector of malaria in sub-Saharan Africa. Herves is transpositionally active and intact copies of it are found in field populations of A gambiae. In this study we report the binding activities of the Herves transposase to the sequences at the ends of the Herves transposon and compare these to other sequences recognized by hAT transposases isolated from other organisms.ResultsWe identified the specific DNA-binding sites of the Herves transposase. Active Herves transposase was purified using an Escherichia coli expression system and bound in a site-specific manner to the subterminal and terminal sequences of the left and right ends of the element, respectively, and also interacted with the right but not the left terminal inverted repeat. We identified a common subterminal DNA-binding motif (CG/AATTCAT) that is critical and sufficient for Herves transposase binding.ConclusionsThe Herves transposase binds specifically to a short motif located at both ends of the transposon but shows differential binding with respect to the left and right terminal inverted repeats. Despite similarities in the overall structures of hAT transposases, the regions to which they bind in their respective transposons differ in sequence ensuring the specificity of these enzymes to their respective transposon. The asymmetry with which the Herves terminal inverted repeats are bound by the transposase may indicate that these differ in their interactions with the enzyme.

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Spread of the autonomous transposable element hobo in the genome of Drosophila melanogaster.

Cited by 9 publications

References 39 publications

Repetitive Element-Mediated Recombination as a Mechanism for New Gene Origination in Drosophila

Repetitive Element-Mediated Recombination as a Mechanism for New Gene Origination in Drosophila

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae

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