Spread of Multidrug-Resistant Bacteria and Antibiotic Resistance Genes in a Swine Wastewater Treatment Plant

de Paula Dias, Camila; Pereira, Andressa Rezende; de Oliveira Paranhos, Aline Gomes; Rodrigues, Marcus Vinícius Duarte; de Lima, Wanderson Geraldo; de Aquino, Sérgio Francisco; de Queiroz Silva, Silvana

doi:10.1007/s41742-023-00552-8

Cited by 1 publication

(1 citation statement)

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“…Antibiotic resistance is an inevitable consequence of the selection pressure exerted on bacteria, and it may swiftly develop and spread in bacteria that adhere to surfaces or form biofilms [1]. Bacterial biofilms consist of a population of sessile microorganisms, which exhibit a substantial increase in resistance to antibiotics, reaching levels up to 1000 times higher than their individual planktonic state, a phenomenon that poses a major barrier in treating and eradicating surface-attached bacteria [2].…”

Section: Introductionmentioning

confidence: 99%

Emerging Issues on Antibiotic-Resistant Bacteria Colonizing Plastic Waste in Aquatic Ecosystems

Ferheen,

Spurio,

Marcheggiani

2024

Antibiotics

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Antibiotic-resistant bacteria (ARB) adhesion onto plastic substrates is a potential threat to environmental and human health. This current research investigates the prevalence of two relevant human pathogens, Staphylococcus spp. and Klebsiella spp., and their sophisticated equipment of antibiotic-resistant genes (ARGs), retrieved from plastic substrates submerged into an inland water body. The results of microbiological analysis on selective and chromogenic media revealed the presence of colonies with distinctive phenotypes, which were identified using biochemical and molecular methods. 16S rDNA sequencing and BLAST analysis confirmed the presence of Klebsiella spp., while in the case of Staphylococcus spp., 63.6% of strains were found to be members of Lysinibacillus spp., and the remaining 36.3% were identified as Exiguobacterium acetylicum. The Kirby–Bauer disc diffusion assay was performed to test the susceptibility of the isolates to nine commercially available antibiotics, while the genotypic resistant profile was determined for two genes of class 1 integrons and eighteen ARGs belonging to different classes of antibiotics. All isolated bacteria displayed a high prevalence of resistance against all tested antibiotics. These findings provide insights into the emerging risks linked to colonization by potential human opportunistic pathogens on plastic waste commonly found in aquatic ecosystems.

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Section: Introductionmentioning

confidence: 99%