Spread of carbapenem-resistant Acinetobacter baumannii carrying a plasmid with two genes encoding OXA-72 carbapenemase in Lithuanian hospitals

Povilonis, Justas; Šeputienė, Vaida; Krasauskas, Renatas; Juškaitė, Raminta; Miškinytė, Monika; Suziedelis, Kestutis; Sužiedėlienė, Edita

doi:10.1093/jac/dks499

Cited by 54 publications

(59 citation statements)

References 28 publications

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“…OXA-72 is a single-amino-acid variant of OXA-24/40 (G224D). In contrast to OXA-23, which is the most common acquired carbapenemase in A. baumannii worldwide, OXA-72-producing isolates have been reported in only a few countries, including Brazil, Colombia, Taiwan, France, Croatia, Poland, Italy, Lithuania, and Sweden (19,21,(23)(24)(25)(26)(27)(28)(29)(30). The clonal spread of OXA-72-producing A. baumannii isolates was previously reported in the Balkan region, in a Croatian university hospital (24).…”

Section: Discussionmentioning

confidence: 88%

First Occurrence of OXA-72-Producing Acinetobacter baumannii in Serbia

Dortet

Bonnin

Bernabeu

et al. 2016

Antimicrob Agents Chemother

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e Here, we characterized the first OXA-72-producing Acinetobacter baumannii isolate (designated MAL) recovered from a urine sample from a Serbian patient. Antimicrobial susceptibility testing, plasmid analysis, and whole-genome sequencing (WGS) were performed to fully characterize the resistome of the A. baumannii MAL clinical isolate. The isolate was multidrug resistant and remained susceptible only to colistin and tigecycline. PCR analysis revealed the presence of the carbapenemase OXA-72, an OXA-40 variant. Extraction by the Kieser method revealed the presence of two plasmids, and one of these, a ca. 10-kb plasmid, harbored the bla OXA-72 gene. WGS revealed 206 contigs corresponding to a genome of 3.9 Mbp in size with a G؉C content of 38.8%. The isolate belonged to sequence type 492 and to worldwide clone II (WWCII). Naturally occurring ␤-lactamase-encoding genes (bla ADC-25 and bla OXA-66 ) were also identified. Aminoglycoside resistance genes encoding one aminoglycoside adenyltransferase (aadA2), three aminoglycoside phosphatases (strA, strB, aphA6), and one 16S RNA methylase (armA) conferring resistance to all aminoglycosides were identified. Resistance to fluoroquinolones was likely due to mutations in gyrA, parC, and parE. Of note, the resistome matched perfectly with the antibiotic susceptibility testing results.

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Section: Discussionmentioning

confidence: 88%

First Occurrence of OXA-72-Producing Acinetobacter baumannii in Serbia

Dortet

Bonnin

Bernabeu

et al. 2016

Antimicrob Agents Chemother

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“…We have performed a PCR-based search for TA systems, validated in this work, with a collection of clinical A. baumannii isolates (n ϭ 476) from Lithuanian hospitals. Most of these isolates belonged to the multidrug-resistant clonal lineages known as European clone I (ECI) and ECII; others were sporadic or nontypeable isolates (N/S) according to a previous pulsed-field gel electrophoresis (PFGE) analysis (24).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, according to our recent observations, these TA modules are harbored by the newly observed ϳ11-kb plasmid pAB120. This small plasmid is prevalent in carbapenem-resistant clinical A. baumannii ECII isolates and carries two copies of the gene for OXA-72 ␤-lactamase, conferring resistance to carbapenems (24). These observations suggest a probable role of plasmid-borne A. baumannii TAs as plasmid stabilization systems contributing to the evolution of antibiotic resistance in this opportunistic pathogen.…”

Section: Figmentioning

confidence: 93%

“…DNAs of A. baumannii isolates (n ϭ 476), recovered from various clinical specimens of patients hospitalized in Lithuanian regional medical centers during the years 2010 and 2011 and genotyped as described previously (24), were used for detection and screening of TA systems. A. baumannii cell lysates were prepared by boiling in water for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…A. baumannii cell lysates were prepared by boiling in water for 10 min. Putative TA genes were detected by PCR using the DNAs of the selected A. baumannii isolates, corresponding to all the genotype groups (24). The screening of validated A. baumannii TAs in the whole collection of clinical A. baumannii isolates was undertaken by PCR using the Tecan Freedom EVO robotic system.…”

Section: Methodsmentioning

confidence: 99%

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Identification and Characterization of Type II Toxin-Antitoxin Systems in the Opportunistic Pathogen Acinetobacter baumannii

Jurenaite

Markuckas

Sužiedėlienė

2013

Journal of Bacteriology

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Acinetobacter baumannii is an opportunistic pathogen that causes nosocomial infections. Due to the ability to persist in the clinical environment and rapidly acquire antibiotic resistance, multidrug-resistant A. baumannii clones have spread in medical units in many countries in the last decade. The molecular basis of the emergence and spread of the successful multidrug-resistant A. baumannii clones is not understood. Bacterial toxin-antitoxin (TA) systems are abundant genetic loci harbored in low-copynumber plasmids and chromosomes and have been proposed to fulfill numerous functions, from plasmid stabilization to regulation of growth and death under stress conditions. In this study, we have performed a thorough bioinformatic search for type II TA systems in genomes of A. baumannii strains and estimated at least 15 possible TA gene pairs, 5 of which have been shown to be functional TA systems. Three of them were orthologs of bacterial and archaeal RelB/RelE, HicA/HicB, and HigB/HigA systems, and others were the unique SplT/SplA and CheT/CheA TA modules. The toxins of all five TA systems, when expressed in Escherichia coli, inhibited translation, causing RNA degradation. The HigB/HigA and SplT/SplA TA pairs of plasmid origin were highly prevalent in clinical multidrug-resistant A. baumannii isolates from Lithuanian hospitals belonging to the international clonal lineages known as European clone I (ECI) and ECII.

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