SPR study for analysis of a water-soluble glycopolymer interface and molecular recognition properties

Glycopolymers Mimicking GM1 Gangliosides: Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition

“…The MW of the glycopolymers were approximately the half of the theoretical value in all polymers. This might due to the unique folded conformation of the glycopolymers which was also indicated in the previous reports …”

“…Generally, polymers immobilized onto a material by adding pre‐synthesized polymer to a material (grafting‐to method) form globule‐like or spread‐out structures called mushroom‐ or pancake‐like structures, respectively . In our previous report, we demonstrated that water‐soluble glycopolymers immobilized onto a gold surface by the grafting‐to method formed mushroom‐like structures in water . Hence, there were small areas on the gold surface where Gal and Neu5Ac were placed close together, similar to the distance between Gal and Neu5Ac in GM1.…”

“…For the kinetic analysis of CTB binding onto the glycopolymers, CTB solution with concentration of 50, 75, 100, 150, 200, 500, and 1000 n m were added to the glycopolymer immobilized gold surfaces and the SPRI signal changes were monitored. The data fitting was performed by the single exponential curve and the binding rate constant k on and dissociation rate constants k off were calculated by relaxation method …”

“…The glycopolymers were synthesized with thiol residues at the termini for easy immobilization onto SPRI gold surfaces. The specific interactions of protein with glycopolymer‐immobilized gold surfaces have already been demonstrated . CTB interactions with the prepared glycopolymer arrays were analyzed by SPRI, and the differences in the molecular recognition properties of various glycopolymers were investigated.…”

“…The specific interactions of protein with glycopolymerimmobilized gold surfaces have already been demonstrated. [20] CTB interactions with the prepared glycopolymer arrays were analyzed by SPRI, and the differences in the molecular recognition properties of various glycopolymers were investigated. Furthermore, the effect of the cooperativity of the carbohydrate modules presented on the polymer side chain on the interaction with CTB was investigated.…”