Spotlight on microRNAs in allergy and asthma

Weidner, Julie; Bartel, Sabine; Kılıç, Ayşe; Zissler, Ulrich M.; Renz, Harald; Schwarze, Jürgen; Schmidt‐Weber, Carsten B.; Maes, Tania; Rebane, Ana; Krauss‐Etschmann, Susanne; Rådinger, Madeleine

doi:10.1111/all.14646

Cited by 108 publications

(79 citation statements)

References 200 publications

(360 reference statements)

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“…The profiling results and the outline of the study are briefly presented in the Additional file 1 . From the miRNAs whose expression significantly differed in asthmatics and controls in the profiling study, we chose those that had been previously tested in serum and were related to the presence of asthma or its clinical features [ 15 , 20 ]. Additionally, although not shown as significant in the profiling results, miRNA-126a-5p was also included in the analysis, as many studies have reported it to have an important role in anti-inflammatory responses [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Circulating miRNA expression in asthmatics is age-related and associated with clinical asthma parameters, respiratory function and systemic inflammation

et al. 2021

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Background The course of asthma may differ between elderly asthmatics (EA) and non-elderly asthmatics (nEA), which may be partially associated with an age-dependent aberrant immune response. The aim of the study was to determine the influence of serum miRNA expression on asthma characteristics and systemic inflammation markers in EA and nEA. Methods Control and severity of asthma, pulmonary function and FeNO were assessed in 28 EA and 31 nEA patients. The control group included 59 elderly and non-elderly healthy individuals. The expression of selected miRNAs in serum was measured with rt-PCR, and proinflammatory cytokine activity was assayed by ELISA or flow cytometry. Results No difference in serum miRNA expression was observed between the asthmatics and healthy controls. EA demonstrated lower expression of miRNA-106a and miRNA-126a than nEA (p = 0.003 and p = 0.02) and EC had lower expression of miRNA-146a, -126a, -106a and 19b than nEC (p = 0.001, p = 0.003, p = 0.005 and p < 0.001 respectively). Only nEA demonstrated a relationship between the expression of selected miRNAs and the level of asthma control (assessed with ACT) and with airway inflammation, measured by FeNO level. All patients with asthma demonstrated elevated TNFα, IL-6 and sTNF RI levels compared to controls (p = 0.026, p = 0.03 and p < 0.001 respectively). EA demonstrated a higher TNFα level than EC (p < 0.001), and EA had a higher level of sTNF RI than nEA (p < 0.001). A significant correlation was observed between serum levels of proinflammatory cytokines and selected miRNAs. Conclusion Serum miRNA expression was found to correlate with clinical characteristics of asthma and systemic inflammation in an age-dependent fashion, suggesting that miRNA may differentially contribute to asthma pathogenesis in elderly and non-elderly patients.

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Section: Methodsmentioning

confidence: 99%

Circulating miRNA expression in asthmatics is age-related and associated with clinical asthma parameters, respiratory function and systemic inflammation

et al. 2021

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“…Alhamwe et al [92] reviewed the role of histone modifications in different allergic conditions including asthma. Additionally, Svitich et al [92] and Weidner et al [93] reviewed the role of miRNAs in asthma and allergy. Recently, Wasti, Liu, and Xiang reviewed the role of different epigenetic classes including histone modifications and miRNA in asthma [94].…”

Section: Epigenomics Of Asthmamentioning

confidence: 99%

Multi-Omics Profiling Approach to Asthma: An Evolving Paradigm

Gautam

Johansson

Mersha

2022

JPM

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Asthma is a complex multifactorial and heterogeneous respiratory disease. Although genetics is a strong risk factor of asthma, external and internal exposures and their interactions with genetic factors also play important roles in the pathophysiology of asthma. Over the past decades, the application of high-throughput omics approaches has emerged and been applied to the field of asthma research for screening biomarkers such as genes, transcript, proteins, and metabolites in an unbiased fashion. Leveraging large-scale studies representative of diverse population-based omics data and integrating with clinical data has led to better profiling of asthma risk. Yet, to date, no omic-driven endotypes have been translated into clinical practice and management of asthma. In this article, we provide an overview of the current status of omics studies of asthma, namely, genomics, transcriptomics, epigenomics, proteomics, exposomics, and metabolomics. The current development of the multi-omics integrations of asthma is also briefly discussed. Biomarker discovery following multi-omics profiling could be challenging but useful for better disease phenotyping and endotyping that can translate into advances in asthma management and clinical care, ultimately leading to successful precision medicine approaches.

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“…In asthmatic patients, the expression of let-7a, and miR-21, -133a, -155, -328 and -1248 in exhaled breath condensates has been shown to be significantly lower than in healthy individuals ( 64 ). Serum miRNA expression is age-dependent and is associated with clinical asthma features and systemic inflammation, suggesting that miRNAs play differential roles in the pathogenesis of asthma in patients from different age groups ( 65 ).…”

Section: Role Of Micrornas In the Pathogenesis Of Pulmonary Fibrosismentioning

confidence: 99%

Current and prospective applications of exosomal microRNAs in pulmonary fibrosis (Review)

et al. 2022

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Pulmonary fibrosis (PF) is a chronic, progressive, irreversible and life-threatening lung disease. However, the pathogenesis and molecular mechanisms of this condition remain unclear. Extracellular vesicles (EVs) are structures derived from the plasma membrane, with a diameter ranging from 30 nm to 5 µ m, that play an important role in cell-to-cell communications in lung disease, particularly between epithelial cells and the pulmonary microenvironment. In particular, exosomes are a type of EV that can deliver cargo molecules, including endogenous proteins, lipids and nucleic acids, such as microRNAs (miRNAs/miRs). These cargo molecules are encapsulated in lipid bilayers through target cell internalization, receptor-ligand interactions or lipid membrane fusion. miRNAs are single-stranded RNA molecules that regulate cell differentiation, proliferation and apoptosis by degrading target mRNAs or inhibiting translation to modulate gene expression. The aim of the present review was to discuss the current knowledge available on exosome biogenesis, composition and isolation methods. The role of miRNAs in the pathogenesis of PF was also reviewed. In addition, emerging diagnostic and therapeutic properties of exosomes and exosomal miRNAs in PF were described, in order to highlight the potential applications of exosomal miRNAs in PF.

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Spotlight on microRNAs in allergy and asthma

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 108 publications

References 200 publications

Circulating miRNA expression in asthmatics is age-related and associated with clinical asthma parameters, respiratory function and systemic inflammation

Circulating miRNA expression in asthmatics is age-related and associated with clinical asthma parameters, respiratory function and systemic inflammation

Multi-Omics Profiling Approach to Asthma: An Evolving Paradigm

Current and prospective applications of exosomal microRNAs in pulmonary fibrosis (Review)

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