Spotlight on fungal pectin utilization—from phytopathogenicity to molecular recognition and industrial applications

Schmitz, Kevin; Protzko, Ryan J.; Zhang, Lisha; Benz, J. Philipp

doi:10.1007/s00253-019-09622-4

Cited by 25 publications

(16 citation statements)

References 157 publications

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“…Economic viability of the fermentative production of biofuels and other valuable chemicals from plant biomass, such as lignocellulosic 1 and pectin-rich 2 residual material, is dependent on the ability of engineered microbial cell factories to efficiently utilize all carbohydrates present in it. Besides glucose, these feedstocks contain large fractions of the pentoses xylose and/or arabinose in varying proportions depending on the biomass type.…”

Section: Introductionmentioning

confidence: 99%

Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity

Rojas

Schadeweg

Kirchner

et al. 2021

Sci Rep

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As abundant carbohydrates in renewable feedstocks, such as pectin-rich and lignocellulosic hydrolysates, the pentoses arabinose and xylose are regarded as important substrates for production of biofuels and chemicals by engineered microbial hosts. Their efficient transport across the cellular membrane is a prerequisite for economically viable fermentation processes. Thus, there is a need for transporter variants exhibiting a high transport rate of pentoses, especially in the presence of glucose, another major constituent of biomass-based feedstocks. Here, we describe a variant of the galactose permease Gal2 from Saccharomyces cerevisiae (Gal2N376Y/M435I), which is fully insensitive to competitive inhibition by glucose, but, at the same time, exhibits an improved transport capacity for xylose compared to the wildtype protein. Due to this unique property, it significantly reduces the fermentation time of a diploid industrial yeast strain engineered for efficient xylose consumption in mixed glucose/xylose media. When the N376Y/M435I mutations are introduced into a Gal2 variant resistant to glucose-induced degradation, the time necessary for the complete consumption of xylose is reduced by approximately 40%. Moreover, Gal2N376Y/M435I confers improved growth of engineered yeast on arabinose. Therefore, it is a valuable addition to the toolbox necessary for valorization of complex carbohydrate mixtures.

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Section: Introductionmentioning

confidence: 99%

Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity

Rojas

Schadeweg

Kirchner

et al. 2021

Sci Rep

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“…A schematic diagram of the structure of viscous β-glucan is shown in Figure 1C. Pectin, a hetero-polysaccharide widely existing in the middle lamella and primary cell walls of the plants, is mainly composed of Dgalacturonic acids linked by an α-1,4-glycosidic bond and contains a small amount of rhamnose, arabinose, and galactose [33]. Gums are high-molecular-weight carbohydrates that at low concentrations can combine with water to form gels.…”

Section: Sdfs Include Many Different Substancesmentioning

confidence: 99%

Soluble Dietary Fiber, One of the Most Important Nutrients for the Gut Microbiota

2021

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Dietary fiber is a widely recognized nutrient for human health. Previous studies proved that dietary fiber has significant implications for gastrointestinal health by regulating the gut microbiota. Moreover, mechanistic research showed that the physiological functions of different dietary fibers depend to a great extent on their physicochemical characteristics, one of which is solubility. Compared with insoluble dietary fiber, soluble dietary fiber can be easily accessed and metabolized by fiber-degrading microorganisms in the intestine and produce a series of beneficial and functional metabolites. In this review, we outlined the structures, characteristics, and physiological functions of soluble dietary fibers as important nutrients. We particularly focused on the effects of soluble dietary fiber on human health via regulating the gut microbiota and reviewed their effects on dietary and clinical interventions.

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“…wood, straw and grasses) (Balat 2011 ). Pectin-rich agricultural residues and agro-food industry residues are potential feedstocks for the production of biofuels and other relevant bioproducts (Schmitz et al 2019 ). Currently, a large fraction of the pectin-rich residues (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…After pre-treatment, the enzymatic or acidic hydrolysis of cellulose, hemicellulose and pectin structures allow the release of monomeric sugars (saccharification) that will subsequently be converted into ethanol and/or other bioproducts by yeasts. Filamentous fungi, in particular Aspergillus sp., Trichoderma reesei and Neurospora crassa , naturally have degrading machinery consisting in hydrolytic and oxidative enzymes which play an important role in plant biomass degradation (Schmitz et al 2019 ). A recent review details the enzyme repertoire of filamentous fungi and their specific transcriptional regulation patterns for efficient biomass degradation (Benocci et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Valorisation of pectin-rich agro-industrial residues by yeasts: potential and challenges

Martins

Monteiro

Semedo

et al. 2020

Appl Microbiol Biotechnol

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Pectin-rich agro-industrial residues are feedstocks with potential for sustainable biorefineries. They are generated in high amounts worldwide from the industrial processing of fruits and vegetables. The challenges posed to the industrial implementation of efficient bioprocesses are however manyfold and thoroughly discussed in this review paper, mainly at the biological level. The most important yeast cell factory platform for advanced biorefineries is currently Saccharomyces cerevisiae, but this yeast species cannot naturally catabolise the main sugars present in pectin-rich agro-industrial residues hydrolysates, in particular Dgalacturonic acid and L-arabinose. However, there are non-Saccharomyces species (non-conventional yeasts) considered advantageous alternatives whenever they can express highly interesting metabolic pathways, natively assimilate a wider range of carbon sources or exhibit higher tolerance to relevant bioprocess-related stresses. For this reason, the interest in nonconventional yeasts for biomass-based biorefineries is gaining momentum. This review paper focuses on the valorisation of pectin-rich residues by exploring the potential of yeasts that exhibit vast metabolic versatility for the efficient use of the carbon substrates present in their hydrolysates and high robustness to cope with the multiple stresses encountered. The major challenges and the progresses made related with the isolation, selection, sugar catabolism, metabolic engineering and use of nonconventional yeasts and S. cerevisiae-derived strains for the bioconversion of pectin-rich residue hydrolysates are discussed. The reported examples of value-added products synthesised by different yeasts using pectin-rich residues are reviewed. Key Points • Review of the challenges and progresses made on the bioconversion of pectin-rich residues by yeasts. • Catabolic pathways for the main carbon sources present in pectin-rich residues hydrolysates. • Multiple stresses with potential to affect bioconversion productivity. • Yeast metabolic engineering to improve pectin-rich residues bioconversion.

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Spotlight on fungal pectin utilization—from phytopathogenicity to molecular recognition and industrial applications

Cited by 25 publications

References 157 publications

Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity

Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity

Soluble Dietary Fiber, One of the Most Important Nutrients for the Gut Microbiota

Valorisation of pectin-rich agro-industrial residues by yeasts: potential and challenges

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