Spot Form of Net Blotch Resistance in a Diverse Set of Barley Lines in Australia and Canada

McLean, Mark S.; Howlett, Barbara J.; Turkington, T. K.; Platz, G. J.; Hollaway, Grant

doi:10.1094/pdis-06-11-0477

Cited by 13 publications

(15 citation statements)

References 25 publications

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“…The distribution of SFNB infection types across the BCC accessions analyzed indicated that susceptibility or resistance is polygenic in nature consistent with previous studies (33,44,68,69). The distribution of SFNB infection types across the BCC accessions analyzed indicated that susceptibility or resistance is polygenic in nature consistent with previous studies (33,44,68,69).…”

Section: Discussionsupporting

confidence: 88%

“…Similarly, NFNB resistance QTL have been identified throughout the genome (33); however, resistance to both forms of the net blotch pathogen were rarely identified in similar locations (20,39), suggesting that the majority of resistances are distinct for the different forms of P. teres and in regards to breeding, the two need to be treated as separate diseases. Growing barley cultivars with genetic resistance to SFNB and NFNB is the most sustainable strategy of disease management (33,44). However, the diversity of virulence/avirulence genes within the pathogen populations and complexity of the interaction with host susceptibility targets and resistance genes requires robust genetic characterization of both the host and pathogen to understand the underlying genetic interactions involved.…”

Section: Discussionmentioning

confidence: 99%

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Association Mapping of Seedling Resistance to Spot Form Net Blotch in a Worldwide Collection of Barley

et al. 2015

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Spot form net blotch (SFNB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. maculata, is an important foliar disease of barley in major production regions around the world. Deployment of adequate host resistance is challenging because the virulence of P. teres f. maculata is highly variable and characterized minor-effect resistances are typically ineffective against the diverse pathogen populations. A world barley core collection consisting of 2,062 barley accessions of diverse origin and genotype were phenotyped at the seedling stage with four P. teres f. maculata isolates collected from the United States (FGO), New Zealand (NZKF2), Australia (SG1), and Denmark (DEN 2.6). Of the 2,062 barley accessions phenotyped, 1,480 were genotyped with the Illumina barley iSelect chip and passed the quality controls with 5,954 polymorphic markers used for further association mapping analysis. Genome-wide association mapping was utilized to identify and map resistance loci from the seedling disease response data and the single nucleotide polymorphism (SNP) marker data. The best among six different regression models was identified for each isolate and association analysis was performed separately for each. A total of 138 significant (-log10P value>3.0) marker-trait associations (MTA) were detected. Using a 5 cM cutoff, a total of 10, 8, 13, and 10 quantitative trait loci (QTL) associated with SFNB resistance were identified for the FGO, SG1, NZKF2, and DEN 2.6 isolates, respectively. Loci containing from 1 to 34 MTA were identified on all seven barley chromosomes with one locus at 66 to 69 cM on chromosome 2H common to all four isolates. Six distinct loci were identified by the association mapping (AM) analysis that corresponded to previously characterized SFNB resistance QTL identified by biparental population analysis (QRpt4, QRpt6, Rpt4, Rpt6, Rpt7, and a QTL on 4H that was not given a provisional gene or QTL nomenclature). The 21 putative novel loci identified may represent a broad spectrum of resistance and or susceptibility loci. This is the first comprehensive AM study to characterize SFNB resistance loci underlying broad populations of the barley host and P. teres f. maculata pathogen.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Association Mapping of Seedling Resistance to Spot Form Net Blotch in a Worldwide Collection of Barley

et al. 2015

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“…One experiment was sown using the conditions described by McLean et al (2010;2012), in which ten replicates of each treatment were sown with two seeds in 5 cm pots containing potting mixture supplemented with trace elements, nitrogen as isobutylidene diurea (IBDU), osmocote fertilizer, lime and iron. Treatments were arranged in a randomised complete block design and seedlings grown in a glasshouse, inoculated to leaf wetness with a spore and mycelium suspension (~2x10 3 parts/ml) of P. teres f. maculata isolate 03-0007 at the 2-3 leaf stage.…”

Section: Glasshouse Experimentsmentioning

confidence: 99%

Spot form of net blotch suppression and yield of barley in response to fungicide application in the Wimmera region of Victoria, Australia

McLean¹,

Weppler²,

Howlett

et al. 2015

Australasian Plant Pathol.

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Spot form of net blotch (SFNB), caused by Pyrenophora teres f. maculata caused up to 25 % (0.9 t/ha) grain yield loss to barley and significant reductions in grain quality parameters such as retention (13 %), screenings (5 %) and grain weight (6 %) in the Wimmera region of Victoria during 2005-11. Spot form of net blotch was suppressed by varying degrees between experiments, seasons, and crop growth stages when foliar fungicide (propiconazole) was applied. Of single applications, treatment at the stem elongation (Z31) or flag leaf (Z39) growth stages generally provided the greatest disease suppression. Single application at tillering (Z25) or ear emergence (Z55) were generally ineffective. Eight chemical seed treatments registered for use in barley were tested as potential low cost management options for SFNB. Four seed treatments reduced SFNB severity in the glasshouse, but none were effective in the field.

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“…Disease management tactics for SFNB consist of fungicide application, inoculum reducing cultural practices, and the introgression of resistance into popular cultivars (22,26). Very little research has been documented to recommend specific barley accessions as effective resistance sources for SFNB.…”

mentioning

confidence: 99%

“…Very little research has been documented to recommend specific barley accessions as effective resistance sources for SFNB. Likewise, McLean et al (22) evaluated the responses of 95 barley accessions and cultivars to SFNB seedling and adult plant resistance in Australia and Canada and found that only two were resistant to all isolates tested, whereas 15 accessions, including cultivars, were resistant to the Canadian isolates tested and a mixture of Australian isolates at the adult plant stage. Likewise, McLean et al (22) evaluated the responses of 95 barley accessions and cultivars to SFNB seedling and adult plant resistance in Australia and Canada and found that only two were resistant to all isolates tested, whereas 15 accessions, including cultivars, were resistant to the Canadian isolates tested and a mixture of Australian isolates at the adult plant stage.…”

mentioning

confidence: 99%

Evaluation of a Barley Core Collection for Spot Form Net Blotch Reaction Reveals Distinct Genotype-Specific Pathogen Virulence and Host Susceptibility

et al. 2015

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Spot form net blotch (SFNB) caused by Pyrenophora teres f. maculata is a major foliar disease of barley (Hordeum vulgare) worldwide. SFNB epidemics have recently been observed in major barley producing countries, suggesting that the local barley cultivars are not resistant and that virulence of the local pathogen populations may have changed. Here we attempt to identify sources of resistance effective against four diverse isolates of P. teres f. maculata collected from around the world. A total of 2,062 world barley core collection accessions were phenotyped using isolates of the pathogen collected in the United States (FGO), Australia (SG1), New Zealand (NZKF2), and Denmark (DEN 2.6). Isolate-specific susceptibility was identified in several of the barley accessions tested, indicating variability in both pathogen virulence and host resistance/susceptibility. Collectively, only 15 barley accessions were resistant across all isolates tested. These resistant accessions will be used to generate mapping populations and for germplasm development. Future research will involve the characterization of host resistance, pathogen virulence, and the host-pathogen interaction associated with SFNB of barley.

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Spot Form of Net Blotch Resistance in a Diverse Set of Barley Lines in Australia and Canada

Cited by 13 publications

References 25 publications

Association Mapping of Seedling Resistance to Spot Form Net Blotch in a Worldwide Collection of Barley

Association Mapping of Seedling Resistance to Spot Form Net Blotch in a Worldwide Collection of Barley

Spot form of net blotch suppression and yield of barley in response to fungicide application in the Wimmera region of Victoria, Australia

Evaluation of a Barley Core Collection for Spot Form Net Blotch Reaction Reveals Distinct Genotype-Specific Pathogen Virulence and Host Susceptibility

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