Sporulation-specific expression of the yvgW (cadA) gene and the effect of blockage on spore properties in Bacillus subtilis

Irigul, Oyku; Karataş, Ayten Yazgan

doi:10.1016/j.gene.2006.06.014

Cited by 1 publication

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References 36 publications

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“…There are also other elements found in bacterial spores that play important roles in stress resistance and in germination. For example, small acid-soluble proteins (SASP) are the predominant spore core proteins and they comprise three different types, a, b, and c [23,24]. SASP have two important functions; one exerted during dormancy and the other during germination.…”

Section: Discussionmentioning

confidence: 99%

Variation of gene expression in Bacillus subtilis samples of fermentation replicates

Zhou

2011

Bioprocess Biosyst Eng

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The application of comprehensive gene expression profiling technologies to compare wild and mutated microorganism samples or to assess molecular differences between various treatments has been widely used. However, little is known about the normal variation of gene expression in microorganisms. In this study, an Agilent customized microarray representing 4,106 genes was used to quantify transcript levels of five-repeated flasks to assess normal variation in Bacillus subtilis gene expression. CV analysis and analysis of variance were employed to investigate the normal variance of genes and the components of variance, respectively. The results showed that above 80% of the total variation was caused by biological variance. For the 12 replicates, 451 of 4,106 genes exhibited variance with CV values over 10%. The functional category enrichment analysis demonstrated that these variable genes were mainly involved in cell type differentiation, cell type localization, cell cycle and DNA processing, and spore or cyst coat. Using power analysis, the minimal biological replicate number for a B. subtilis microarray experiment was determined to be six. The results contribute to the definition of the baseline level of variability in B. subtilis gene expression and emphasize the importance of replicate microarray experiments.

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Section: Discussionmentioning

confidence: 99%

Variation of gene expression in Bacillus subtilis samples of fermentation replicates

Zhou

2011

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

Sporulation-specific expression of the yvgW (cadA) gene and the effect of blockage on spore properties in Bacillus subtilis

Cited by 1 publication

References 36 publications

Variation of gene expression in Bacillus subtilis samples of fermentation replicates

Variation of gene expression in Bacillus subtilis samples of fermentation replicates

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