Sporulation of Bacillus spp. within biofilms: A potential source of contamination in food processing environments

Faille, Christine; Bénézech, Thierry; Midelet, Graziella; Lequette, Yannick; Clarisse, Martine; Ronse, Gilles; Ronse, Annette; Slomianny, Christian

doi:10.1016/j.fm.2013.12.004

Cited by 117 publications

(77 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…cereus is an industrial contaminant and a public health hazard widespread in nature and frequently isolated from dairy products and equipment (Blel et al, 2008;Faille et al, 2014;Lee et al, 2010;Nam et al, 2014;Peng et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…The substrate loading rate also affects strongly the transport of the substrate to the cells, therefore influencing their metabolic growth, the production of extracellular polymeric substance (EPS) and ultimately the structure of the biofilm (Vieira et al, 1993). B. cereus is an industrial contaminant and a public health hazard widespread in nature and frequently isolated from dairy products and equipment (Blel et al, 2008;Faille et al, 2014;Lee et al, 2010;Nam et al, 2014;Peng et al, 2002). …”

mentioning

confidence: 99%

See 1 more Smart Citation

The effect of shear stress on the formation and removal of Bacillus cereus biofilms

Lemos

Mergulhão

Melo

et al. 2015

Food and Bioproducts Processing

View full text Add to dashboard Cite

A b s t r a c tThe influence of the shear stress (rw) under which biofilms were formed was assessed on their susceptibility to removal when exposed to chemical and mechanical stresses. A rotating cylinder reactor was used to form biofilms, allowing the simulation of rw conditions similar to those found in industrial settings, particularly in areas with low rw like elbows, corners, valves and dead zones. Bacillus cereus was used as a model bacterium for biofilm formation.Biofilms were formed on AISI316 stainless steel cylinders under different rw (estimated at 0.02, 0.12 and 0.17 Pa) for 7 days. Some phenotypic characteristics, including thickness, biomass production, cellular density and extracellular proteins and polysaccharides content were assessed. Biofilm density was found to increase significantly with rw while the thickness decreased. Also, biofilms formed at 0.02 Pa had lowest biomass content, cell density and extracellular polysaccharide content. Those characteristics were not statistically different for the biofilms formed under 0.12 and 0.17 Pa.Ex situ tests were performed by treating the biofilms with the biocide benzyldimethyldodecyl ammonium chloride (BDMDAC), followed by exposure to increasing rw conditions, up to 1.84 Pa (whereas the maximum rw used during growth was 0.17 Pa). The biofilms formed under low rw were more resistant to removal caused by the BDMDAC action alone. Those formed under higher rw were more resistant to the mechanical and the combined chemical and mechanical treatments. The amount of biofilm remaining on the cylinders, after both treatments was statistically similar for biofilms formed under 0.12 and 0.17Pa. The resistance of biofilms to removal by mechanical treatment (alone and combined with BDMDAC) was related to the amount of matrix polysaccharides.However, none of the methods investigated were able to remove all the biofilm from the cylinders. IntroductionThere is a lack of efficient strategies to clean stagnant zones in industrial plants (Brooks and Flint, 2008). Crevices, corners, dead zones, valves or areas where the mixing rate is low are almost inevitable. Stagnation promotes bacterial accumulation, ultimately leading to biofouling (Manuel et al., 2010). Biofouling is a damaging problem, affecting the energetic efficiency of industrial processes, causing corrosion of the surfaces, decreasing product quality and eventually promoting the spread of pathogens and resistant infectious diseases (Costerton et al., 1999;Ludensky, 2003;Srey et al., 2013). In industrial settings, surface disinfection is usually focused on the use of biocides, aiming to inactivate the microorganisms (Cloete et al., 1998;Faille et al., 2013). Since biofilms are complex biological structures adhered to a sur-face, these strategies often fail, as the removal of biomass is neglected. Hence, cleaning the biomass from the surfaces is fundamental for controlling biofilm development (Flemming, 2011).In the biofilm formation process, the hydrodynamic conditions define the transport of the cells, oxyg...

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

The effect of shear stress on the formation and removal of Bacillus cereus biofilms

Lemos

Mergulhão

Melo

et al. 2015

Food and Bioproducts Processing

View full text Add to dashboard Cite

show abstract

“…Biofilms are a possible cause of recontamination in food production and should be considered in development of hazard analysis and critical control point plans (HACCP) Anand 2002, Shi andZhu 2009). It is generally assumed that (re)contamination originating from biofilms is a major determinant affecting food quality and safety (Faille et al 2014). Besides the recontamination risk, biofilms are undesired in processing environments since their presence may also cause corrosion and equipment inefficiencies (Kumar and Anand 1998).…”

Section: Microbial Biofilmsmentioning

confidence: 99%

“…Attached spores may germinate, grow out and eventually lead to the development of mature biofilms when favourable conditions are encountered (Wijman et al 2007). Within established biofilms, B. cereus is able to form spores (Lindsay et al 2006, Wijman et al 2007, Faille et al 2014, which forms an additional route of contamination via release of spores from the biofilm and thus close the vicious cycle, as depicted in Fig. 2.…”

Section: Influence Of Environmental Factors: Iron Sources and Biofilmmentioning

confidence: 99%

“…Different studies report biofilm formation by B. cereus on abiotic surfaces including stainless steel, polystyrene and glass (Auger et al 2006, Lindsay et al 2006, Wijman et al 2007, Houry et al 2010, Karunakaran and Biggs 2011) but a direct comparison between different substrata has not been performed. Within the established biofilms, B. cereus is able to form spores (Lindsay et al 2006, Wijman et al 2007, Faille et al 2014 which may lead to product contamination upon release. The objective of this study was to assess the biofilm forming capacity of 21 B. cereus food isolates and two reference strains, ATCC 14579 and ATCC 10987 on stainless steel and polystyrene and to evaluate the influence of iron.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacillus cereus growth and biofilm formation: the impact of substratum, iron sources, and transcriptional regulator Sigma 54

Hayrapetyan¹

View full text Add to dashboard Cite

Overview of the Problems Resulting from Biofilm Contamination in the Dairy Industry

Seale¹,

Bremer²,

Flint³

et al. 2015

Biofilms in the Dairy Industry

View full text Add to dashboard Cite

Sporulation of Bacillus spp. within biofilms: A potential source of contamination in food processing environments

Cited by 117 publications

References 53 publications

The effect of shear stress on the formation and removal of Bacillus cereus biofilms

The effect of shear stress on the formation and removal of Bacillus cereus biofilms

Bacillus cereus growth and biofilm formation: the impact of substratum, iron sources, and transcriptional regulator Sigma 54

Overview of the Problems Resulting from Biofilm Contamination in the Dairy Industry

Contact Info

Product

Resources

About