Sporulation activated via σ<sup>W</sup> protects <i>Bacillus</i> from a Tse1 peptidoglycan hydrolase T6SS effector

Pérez-Lorente, Alicia Isabel; Molina-Santiago, Carlos; Vicente, Antonio de; Romero, Diego

doi:10.1101/2022.02.23.481616

Cited by 4 publications

(2 citation statements)

References 88 publications

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“…Pairwise interactions between bacilli and pseudomonads might therefore play a crucial role in determining plant growth, health, and local microbiome composition. Studies investigating such pairwise interactions are revealing more detail, outlining the molecular mechanisms, and determining the degree to which interactions and their effector molecules are conserved [21][22][23][24][25]. However, despite these advances, the large bioactive potential of Bacillus and Pseudomonas makes mapping the overarching interaction mechanisms challenging, and a consensus has not been reached on the general interactions between members of these two genera.…”

Section: Introductionmentioning

confidence: 99%

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Lyng

Jørgensen

Schostag

et al. 2023

Preprint

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Siderophores have long been implicated in sociomicrobiology as determinants of bacterial interrelations. For plant-associated genera like Bacillus and Pseudomonas, siderophores are well known for their biocontrol functions. Here, we explored the functional role of the Bacillus subtilis siderophore bacillibactin in an antagonistic interaction with Pseudomonas marginalis. The presence of bacillibactin strongly influenced the outcome of the interaction in an iron-dependent manner. The bacillibactin producer B. subtilis restricts colony spreading of P. marginalis by repressing the transcription of histidine kinase-encoding gene gacS, thereby abolishing production of secondary metabolites such as pyoverdine and viscosin. By contrast, lack of bacillibactin restricted B. subtilis colony growth in a mechanism reminiscent of a siderophore tug-of-war for iron. Our analysis revealed that the Bacillus-Pseudomonas interaction is conserved across fluorescent Pseudomonas spp., expanding our understanding of the interplay between two genera of the most well-studied soil microbes.

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Section: Introductionmentioning

confidence: 99%

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Lyng

Jørgensen

Schostag

et al. 2023

Preprint

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“…Eco upregulates its envelope stress responses Rcs and BaeSR after exposure to the Vibrio cholerae (V52) T6SS effectors TseH (a PG hydrolase) [21] and TseL (a lipase) [22], suggesting that Eco could counter cell envelope damage by re-enforcing its surface [23]. Similarly, Bacillus subtilis triggers protective sporulation in response to a Pseudomonas chlororaphis (PCL1606) T6SS effector, Tse1 (a muramidase) [24]. Additional recipient-cell coordinators of T6SS effector toxicity include reactive oxygen species [25] and glucose-dependent gene expression [26].…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide transport regulates bacterial sensitivity to a cell wall-degrading intermicrobial toxin

et al. 2023

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Gram-negative bacteria can antagonize neighboring microbes using a type VI secretion system (T6SS) to deliver toxins that target different essential cellular features. Despite the conserved nature of these targets, T6SS potency can vary across recipient species. To understand the functional basis of intrinsic T6SS susceptibility, we screened for essential Escherichia coli (Eco) genes that affect its survival when antagonized by a cell wall-degrading T6SS toxin from Pseudomonas aeruginosa, Tae1. We revealed genes associated with both the cell wall and a separate layer of the cell envelope, lipopolysaccharide, that modulate Tae1 toxicity in vivo. Disruption of genes in early lipopolysaccharide biosynthesis provided Eco with novel resistance to Tae1, despite significant cell wall degradation. These data suggest that Tae1 toxicity is determined not only by direct substrate damage, but also by indirect cell envelope homeostasis activities. We also found that Tae1-resistant Eco exhibited reduced cell wall synthesis and overall slowed growth, suggesting that reactive cell envelope maintenance pathways could promote, not prevent, self-lysis. Together, our study reveals the complex functional underpinnings of susceptibility to Tae1 and T6SS which regulate the impact of toxin-substrate interactions in vivo.

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Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics

Qin

Xiao

Zhou

et al. 2022

Sig Transduct Target Ther

436

323

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Pseudomonas aeruginosa (P. aeruginosa) is a Gram-negative opportunistic pathogen that infects patients with cystic fibrosis, burn wounds, immunodeficiency, chronic obstructive pulmonary disorder (COPD), cancer, and severe infection requiring ventilation, such as COVID-19. P. aeruginosa is also a widely-used model bacterium for all biological areas. In addition to continued, intense efforts in understanding bacterial pathogenesis of P. aeruginosa including virulence factors (LPS, quorum sensing, two-component systems, 6 type secretion systems, outer membrane vesicles (OMVs), CRISPR-Cas and their regulation), rapid progress has been made in further studying host-pathogen interaction, particularly host immune networks involving autophagy, inflammasome, non-coding RNAs, cGAS, etc. Furthermore, numerous technologic advances, such as bioinformatics, metabolomics, scRNA-seq, nanoparticles, drug screening, and phage therapy, have been used to improve our understanding of P. aeruginosa pathogenesis and host defense. Nevertheless, much remains to be uncovered about interactions between P. aeruginosa and host immune responses, including mechanisms of drug resistance by known or unannotated bacterial virulence factors as well as mammalian cell signaling pathways. The widespread use of antibiotics and the slow development of effective antimicrobials present daunting challenges and necessitate new theoretical and practical platforms to screen and develop mechanism-tested novel drugs to treat intractable infections, especially those caused by multi-drug resistance strains. Benefited from has advancing in research tools and technology, dissecting this pathogen’s feature has entered into molecular and mechanistic details as well as dynamic and holistic views. Herein, we comprehensively review the progress and discuss the current status of P. aeruginosa biophysical traits, behaviors, virulence factors, invasive regulators, and host defense patterns against its infection, which point out new directions for future investigation and add to the design of novel and/or alternative therapeutics to combat this clinically significant pathogen.

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Sporulation activated via σ^W protects Bacillus from a Tse1 peptidoglycan hydrolase T6SS effector

Cited by 4 publications

References 88 publications

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Lipopolysaccharide transport regulates bacterial sensitivity to a cell wall-degrading intermicrobial toxin

Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics

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Sporulation activated via σW protects Bacillus from a Tse1 peptidoglycan hydrolase T6SS effector

Cited by 4 publications

References 88 publications

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Lipopolysaccharide transport regulates bacterial sensitivity to a cell wall-degrading intermicrobial toxin

Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics

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Sporulation activated via σ^W protects Bacillus from a Tse1 peptidoglycan hydrolase T6SS effector