Sporotrichosis

Urabe, Harukuni; Honbo, Shozo

doi:10.1111/j.1365-4362.1986.tb02238.x

Cited by 29 publications

(14 citation statements)

References 2 publications

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“…S. schenckii is a dimorphic fungus, but mainly the yeast form of cell growth was observed in all three liquid media. Production of proteinases in the yeast form of the organism coincides with the clinical observation that the yeast form of the organism is predominant in affected tissues (15,25). Isolated extracellular proteinases I and II were demonstrated to be homogeneous by the elution pattern of gel filtration, PAGE, and isoelectric focusing.…”

Section: Discussionsupporting

confidence: 62%

“…Sporotrichosis, a fungus infection caused by Sporothrix schenckii, is widely distributed throughout the world (15) with approximately 150 cases being reported annually in Japan (25). S. schenckii from the soil invades the skin and produces nodules and ulcers along the lymphatics.…”

mentioning

confidence: 99%

“…S. schenckii from the soil invades the skin and produces nodules and ulcers along the lymphatics. S. schenckii is a dimorphic fungus, but the yeast form is dominant in affected tissues and is believed to be more virulent (15,25). However, factors related to the invasion of the parasite which lead to the instigation of these lesions have not yet been clarified.…”

mentioning

confidence: 99%

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Isolation and properties of extracellular proteinases from Sporothrix schenckii

Tsuboi¹,

T²,

Takamori³

et al. 1987

J Bacteriol

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Sporothrix schenckii, mainly in the yeast form of the organism, produced extracelluilar proteinases when cultivated in liquid media containing albumin or collagen as a nitrogen source, but did not do so in brain heart infusion medium. Isolation of two extracellular proteinases from albumin-containing medium was performed by chromatography on DEAE-Sepharose CL-6B and Sephacryl S-200. Proteinase I had a molecular weight of 36,500, an optimal pH at 6.0, and a pI at 4.8. Despite its activities in weakly acidic conditions, proteinase I demonstrated chymotrypsinlike characteristics, these being .indicated by strong inhibitory activity by phenylmethylsulfonyl fluoride and chymostatin and good kinetic constants for a synthetic chymotrypsin substrate, Suc-Ala-Ala-Pro-Phe-MCA. Proteinase II had a molecular weight of 39,000, an optimal pH at 3.5, and a pl at 3.8. Proteinase II showed cathepsin D-like characteristics, these being indicated by strong inhibitory activity by pepstatin, an acidic optimal pH, and good kinetic constants for hemoglobin. These two enzymes hydrolyzed natural substrates such as stratum corneum, type I collagen, and elastin although not type IV collagen. Proteinase production and cell growth in collagen-containing medium and the enzymatic digestion of skin constituents by isolated proteinases suggested that these two proteinases cooperatively enable the organism to invade skin and to obtain peptides from insoluble proteins. proteinases, 200-ml samples of BSA-containing media in 500-ml flasks were incubated under the same conditions as described above for 1 week.Assay of proteinase activity. Proteinase activity was assayed with Azocoll (Sigma) as a substrate. A 5-mg sample of Azocoll in 50 mM citric acid-100 mM disodium phosphate buffer was incubated with 50 to 100 ,ul of enzyme solution (total volume, 1.0 ml) for 1 h at 37°C. Incubation was terminated by the addition of 2 ml of cold distilled water. After centrifugation at 1,500 x g for 10 min, released dye in the supernatant was measured at 520 nm with a Hitachi spectrophotometer (model 100-60). One unit of proteinase activity was defined as the amount of enzyme which produced an increase in absorbance of 0.10 under the abovementioned conditions. Determination of protein concentration. Protein concentration was determined by the method of Lowry et al. (9) (Amicon Corp., Lexington, Mass.). After centrifugation at 1,500 x g for 15 min, the supernatant was applied to a DEAE-Sepharose CL-6B column (1.2 by 11 cm) which had been previously equilibrated with the same 4104

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Section: Discussionsupporting

confidence: 62%

mentioning

confidence: 99%

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Isolation and properties of extracellular proteinases from Sporothrix schenckii

Tsuboi¹,

T²,

Takamori³

et al. 1987

J Bacteriol

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“…Als Infektionsmodus werden am häufigsten Verletzungen durch Pflanzenstachel oder Insekten angegeben [11]. Die Erkrankung kann jedoch offenbar auch als Zoonose übertragen werden [6].…”

Section: Besprechungunclassified

“…Da optimale Wachtstumbedingungen für den Erreger bei Temperaturen unter 37 °C bestehen, stellt offenbar auch die lokale Thermotherapie eine effektive Behandlungsmethode dar [11]. Heute ist der Einsatz von Itraconazol in einer Tagesdosis von 1-2 x 100 mg über 3 Monate zu empfehlen.…”

Section: Therapieunclassified