Sporolactobacillus pectinivorans sp. nov., an anaerobic bacterium isolated from spoiled jelly

Lan, Quan Xue; Chen, Jing; Lin, Lin; Ye, Xiu Ling; Yan, Qiong Ying; Huang, Jian; Liu, Cong Cong; Yang, Guo Wu

doi:10.1099/ijsem.0.001351

Cited by 10 publications

(3 citation statements)

References 18 publications

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“…Other species have since been isolated from soil or the rhizosphere of plants [ 32 – 35 ]. In recent years, novel species of Sporolactobacillus have been cultured from decaying foodstuffs such as spoiled orange juice ( S. putidus ) [ 36 ] and spoiled jelly ( S. pectinovorans ) [ 37 ], but to our knowledge there are no publications on the role of Sporolactobacillus in the gut microbiota. Because of its lactic acid properties S. inulinus has been suggested as a possible probiotic [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

The relation between Blastocystis and the intestinal microbiota in Swedish travellers

et al. 2017

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Background Blastocystis sp. is a unicellular eukaryote that is commonly found in the human intestine. Its ability to cause disease is debated and a subject for ongoing research. In this study, faecal samples from 35 Swedish university students were examined through shotgun metagenomics before and after travel to the Indian peninsula or Central Africa. We aimed at assessing the impact of travel on Blastocystis carriage and seek associations between Blastocystis and the bacterial microbiota.ResultsWe found a prevalence of Blastocystis of 16/35 (46%) before travel and 15/35 (43%) after travel. The two most commonly Blastocystis subtypes (STs) found were ST3 and ST4, accounting for 20 of the 31 samples positive for Blastocystis. No mixed subtype carriage was detected. All ten individuals with a typable ST before and after travel maintained their initial ST. The composition of the gut bacterial community was not significantly different between Blastocystis-carriers and non-carriers. Interestingly, the presence of Blastocystis was accompanied with higher abundances of the bacterial genera Sporolactobacillus and Candidatus Carsonella. Blastocystis carriage was positively associated with high bacterial genus richness, and negatively correlated to the Bacteroides-driven enterotype. These associations were both largely dependent on ST4 – a subtype commonly described from Europe – while the globally prevalent ST3 did not show such significant relationships.ConclusionsThe high rate of Blastocystis subtype persistence found during travel indicates that long-term carriage of Blastocystis is common. The associations between Blastocystis and the bacterial microbiota found in this study could imply a link between Blastocystis and a healthy microbiota as well as with diets high in vegetables. Whether the associations between Blastocystis and the microbiota are resulting from the presence of Blastocystis, or are a prerequisite for colonization with Blastocystis, are interesting questions for further studies.Electronic supplementary materialThe online version of this article (10.1186/s12866-017-1139-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

The relation between Blastocystis and the intestinal microbiota in Swedish travellers

et al. 2017

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“…In addition, Sporolactobacillus vineae was isolated from vineyard soil in Korea [9], and Sporolactobacillus putidus isolated from a spoiled orange juice was proposed [10]. Sporolactobacillus shorae and Sporolactobacillus spathodeae from tree barks in Thailand [11] and Sporolactobacillus pectinivoran s from spoiled jelly in China were discovered [12]. Sporolactobacillus shoreicorticis was recently isolated from tree bark in northeast Thailand and assigned to a novel species [13].…”

Section: Introductionmentioning

confidence: 99%

Sporolactobacillus mangiferae sp. nov., a spore-forming lactic acid bacterium isolated from tree bark in Thailand

Phuengjayaem

Poothong

Sitdhipol

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, facultatively anaerobic and endospore-forming rod-shaped bacterium, designed strain CPB3-1T, was isolated from tree bark. This homofermentative strain produced dl-lactic acid from glucose. It grew at 20–45 °C, pH 4.0–9.5 and in 0-3.0 % (w/v) NaCl. It contained meso-diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant component. The major fatty acid was anteiso-C17 : 0. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown lipid. Based on the results of 16S rRNA gene sequence analysis, strain CPB3-1T belonged to the genus Sporolactobacillus and was closely related to Sporolactobacillus kofuensis DSM 11701T and Sporolactobacillus spathodeae BK117-1T (both 96.7 % similarity), Sporolactobacillus inulinus NRIC 1133T and Sporolactobacillus terrae DSM 11697T (both 96.6 % similarity), and Sporolactobacillus shoreicorticis MK21-7T, Sporolactobacillus laevolacticus DSM 442T, Sporolactobacillus shoreae BK92T and Sporolactobacillus pectinivorans GD201205T (all 95.8–96.5 % similarity). The draft genome of strain CPB3-1T contained 2 930 919 bps with 3117 coding genes. The DNA G+C content was 45.1 mol%. The digital DNA–DNA hybridization values between strain CPB3-1T and closely related type strains were 19.2–24.0 %. The average nucleotide identity (84.0–87.6 %) and average amino acid identity (66.5–76.3 %) values were lower than the cut-off values for species delineation. Strain CPB3-1T was clearly distinguished from related Sporolactobacillus species based on its phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence similarity and the results of draft genome analysis. Therefore, the strain represents a novel species of the genus Sporolactobacillus , for which the name Sporolactobacillus mangiferae sp. nov. is proposed. The type strain is CPB3-1T (=JCM 35082T=TISTR 10004T).

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“…racemicus, S. terrae, S. kofuensis, S. lactosus and S. laevolacticus were isolated from soil, and fermentation starters were proposed by Yanagida et al (1997). In addition, S. vineae, S. putidus and S. pectinivorans were isolated from vineyard soil in Korea, spoiled orange juice and spoiled jelly, respectively Fujita et al, 2010;Lan et al, 2016). Sporolactobacillus spathodeae and Sporolactobacillus shoreae were isolated from tree barks in Thailand (Thamacharoensuk et al, 2015).…”

Section: Genus Lactobacillusmentioning

confidence: 99%

Screening of spore forming d-lactic acid producing bacteria and its optimization

Prasirtsak

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In this study, isolation, screening, fermentation optimization, and determination of the expression of key enzymes involving in D-lactic acid production were conducted for selection of the novel D-lactic acid isolate with the high yield, productivity, and optical purity. From the screening experiment, the novel isolate, NK26-11T, was obtained. The isolate NK26-11T was a Gram-stain-positive, catalase-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium isolated from soil sample in Thailand. This isolate homofermentatively fermented glucose for D-lactic acid and grew at the wide range of temperature between 20 and 45 ?C and the pH of 5-8.5. The cell-wall peptidoglycan of NK26-11T contained meso-diaminopimelic acid. The major respiratory quinone was menaquinone 7 (MK-7), the DNA G+C content was 42.6 mol%, and the major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. On the basis of 16S rRNA gene sequences analysis, the isolate NK26-11T was closely related to Bacillus solimangrovi JCM 18994T (93.89% 16S rRNA gene sequence similarity), Pullulanibacillus naganoensis LMG 12887T (93.32%), Sporolactobacillus inulinus NRIC 1133T (92.99%), Tuberibacillus calidus JCM 13397T (92.98%) and Thalassobacillus devorans DSM 16966T (?90.93%). The isolate NK26-11T represents a novel species of a new genus between Bacillus and Sporolactobacillus clusters, for which the name Terrilactibacillus laevilacticus gen. nov., sp. nov. was proposed. The type strain of the type species is NK26-11T (=LMG 27803T =TISTR 2241T =PCU 335T). From the fermentation screening of the selected D-lactic acid producing isolates, it was found that Terrilactibacillus laevilacticus SK5-6 exhibited a good D-lactate production performance (99.27 g/L final lactate titer with 0.90 g/g yield, 1.38 g/L.h, and 99.00% D-enantiomer equivalent) compared with other Sporolactobacillus sp. and Terrilactibabacillus sp. This isolate could ferment a wide range of sugars for D-lactic acid. Unlike the typical D-lactic acid producers, such as catalase negative Sporolactobacillus sp., T. laevilacticus SK5-6 acquired catalase activity; therefore, a 2-phase fermentation was simply employed for D-lactic acid production. Under an aerobic preculture stage, high-cell-density biomass was rapidly obtained as the result of aerobic respiration. At the correct physiological stage (inoculum age) and a proper concentration of biomass (inoculum size) transferred to the fermentation stage, SK5-6 rapidly converted glucose into D-lactic acid under anaerobic conditions resulting in a high final lactic acid titer (102.22 g/L), yield (0.84 g/g), and productivity (2.13 g/L.h) without byproduct formation. It was found that SK5-6 exhibited both fermentation kinetic and expression level of the key enzymes higher than those of S. laevilacticus, a catalase negative D-lactate producer. Low phosphofructokinase activity revealed that glycolysis controlled the apparent D-lactic acid productivity by SK5-6. Increasing the pH during fermentation phase activated the activity of D-LDH (D-lactate dehydrogenase) beyond that of L-LDH, resulting in the high optical purity of D-lactate, while the acidic pH promoted the activities of the kinases in glycolysis. The conversion of L-lactate to D-lactate by isomerase was also observed during fermentation. The findings in this study demonstrated the remarkable characteristics of SK5-6, in particular, a high product yield was obtained without byproduct formation. From the key characteristics of SK5-6, this isolate can be claimed as an industrial D-lactic acid producer.

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Sporolactobacillus pectinivorans sp. nov., an anaerobic bacterium isolated from spoiled jelly

Cited by 10 publications

References 18 publications

The relation between Blastocystis and the intestinal microbiota in Swedish travellers

The relation between Blastocystis and the intestinal microbiota in Swedish travellers

Sporolactobacillus mangiferae sp. nov., a spore-forming lactic acid bacterium isolated from tree bark in Thailand

Screening of spore forming d-lactic acid producing bacteria and its optimization

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