Sporogenesis in Eusporangiate Ferns: II. Polyplastidic Meiosis in Ophioglossum (Ophioglossales)

Abstract

Structural studies of sporogenesis using combined methods of immunofluorescence labelling of microtubules and transmission electron microscopy provide details of meiosis and cytokinesis in Ophiog/ossum petiolatum. Unlike Angiopteris (Marattiales), which is monoplastidic, Ophiog/lossum undergoes polyplastidic meiosis like members of the fern-seed plant clade. The meiotic spindle is distinctly multipolar in origin and is consolidated into a bipolar spindle that is variously twisted and curved to accommodate the large number of chromosomes. Although a phragmoplast forms after first meiosis, no wall is deposited. Instead, an organelle band consisting of intermingled plastids and mitochondria is formed in the equatorial region between the dyad domains. Following second meiosis, a complex of phragmoplasts forms among sister and non-sister nuclei. Cell plates are deposited first between sister nuclei and then in the region of the organelle band resulting in a tetrad of spores each with a equal allotment of organelles.

Cell Biological Characterization of Male Meiosis and Pollen Development in Rice

“…The formation of cell plate in rice instead of an organelle band at the end of meiosis I may provide a way to study the reproduction and segregation of organelles. This clear organelle band occurs during meiosis in many plants, including ferns (Brown and Lemmon 2001), suggesting that it is a conserved process. The fact that rice is different from these others plants allows comparison studies of organelle regeneration and segregation.…”

Distribution of plastids and mitochondria during male gametophyte formation in Tinantia erecta (Jacq.) Fenzl

“…This type of chondriokinesis was also observed in Larix europea (Rodkiewicz et al 1984; Bednara and Rodkiewicz 1988), Larix decidua (Rodkiewicz and Duda 1988), Trandescantia virginica (Rodkiewicz et al 1984), and ”Vista Rainbow” orchid (Brown and Lemmon 1991). The neutral-equatorial type of chondriokinesis was also observed in the microsporogenesis with simultaneous cytokinesis in Chamaedorea karwinskiana (Suessenguth 1921), Tropaeolum peregrinum (Sugiura 1928), Ribes rubrum (Geneves 1967, 1971), Podocarpus macrophylla (Vasil and Aldrich 1970), Paeonia tenuifolia and Campanula rapanculoides (Dietrich 1973), Pteridium aquilinum (Sheffield and Bell 1979), Dryopteris borreri (Sheffield and Bell 1979; Sheffield et al 1983), Lycopersicon peruvianum (Pacini and Juniper 1984), Datura inoxa , Nicotiana tabacum and Antirrhinum majus (Dupuis et al 1988), Solanum nigrum (Bhandari and Sharma 1988), Polystichum loncitis (Bednara and Rodkiewicz 1988), “Sabine Queen” orchid (Brown and Lemmon 1991), Lilium longiflorum (Dickinson and Heslop-Harrison 1970; Tanaka 1991), Ophioglossum petiolatum (Brown and Lemmon 2001a, b), Psilotum nudum (Gabarayeva 1985; Lee 1982; Tchórzewska et al 1996; Tchórzewska and Bednara 2011), Ginkgo biloba (Wolniak 1976; Wang et al 1988; Brown and Lemmon 2005), Taranna gracilipes (Vinckier and Smets 2007), Armoracia rusticana (Winiarczyk et al 2007), and Arabidopsis thaliana (Brownfield et al 2015).…”

Ontogenia y aspectos histoquímicos de los esporangios y escamas receptáculares del helecho epífito Pleopeltis macrocarpa (Polypodiaceae)

“…Se verificó además en esta investigación que para P. macrocarpa, la banda de orgánulos cumple una función importante indicando el lugar exacto del depósito de la placa celular y por consiguiente en la forma como se organizan las esporas al interior de la tétrada. Estas observaciones están en concordancia con los hallazgos hechos por otros autores (Sheffield & Bell, 1979;Sheffield et al, 1983;Brown & Lemmon, 2001a, 2001bRincón et al, 2013).…”

“…(Marattiales) concluyendo que esta especie presenta meiosis monoplastídica. Por el contrario, a la meiosis poliplastidial que describieron durante la esporogénesis de Ophioglossum L. (Ophioglossales) (Brown & Lemmon 2001b). Por otro lado, Rincón, Forero, Gélvez, Torres y Rolleri (2011) describen el desarrollo del estróbilo y la esporogénesis en Equisetum giganteum L. de ejemplares recolectados en Colombia.…”