Spores germination and gametophytes of Alsophila odonelliana (Cyatheaceae) in different sterile media

Bonomo, M. C.; Martinez, O. M. M.; Tanco, M. E.; Cardozo, Rojas; Avilés, Zulma

doi:10.32604/phyton.2013.82.119

Cited by 7 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The advantage of spore culture over tissue culture for the conservation of rare species is that it retains the genetic variability inherited in the genetic makeup of the species (Marimuthu & Manickam, 2011). In many fern species, multiplication of gametophytes increased when the medium was supplemented with PGRs under in vitro conditions (Bharati et al, 2013; Bonomo et al, 2013; Das et al, 2013; Ravi et al, 2014). Similar findings were made by Bonomo et al (2013) while studying Alsophila odonelliana (Alston) Lehnert (Cyatheaceae), who discovered that MS media supplemented with BAP caused filamentous gametophytes to grow longitudinally and proliferate from early stages, whereas 2,4‐D caused short filamentous gametophytes to develop.…”

Section: Discussionmentioning

confidence: 99%

“…In many fern species, multiplication of gametophytes increased when the medium was supplemented with PGRs under in vitro conditions (Bharati et al, 2013; Bonomo et al, 2013; Das et al, 2013; Ravi et al, 2014). Similar findings were made by Bonomo et al (2013) while studying Alsophila odonelliana (Alston) Lehnert (Cyatheaceae), who discovered that MS media supplemented with BAP caused filamentous gametophytes to grow longitudinally and proliferate from early stages, whereas 2,4‐D caused short filamentous gametophytes to develop. In various studies, gametophytes were regarded as the most suitable material for large‐scale propagation of fern sporophytes (Johari & Singh, 2018; Singh et al, 2013, 2017).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro development of gametophytes and sporophytes of critically endangered fern Christella kendujharensis S.K. Behera & S.K. Barik (Thelypteridaceae)

2023

View full text Add to dashboard Cite

Christella kendujharensis S.K. Behera & S.K. Barik (family: Thelypteridaceae) is a critically endangered fern endemic to India. We developed its mass propagation protocol through in vitro spore culture in a liquid suspended medium for germination of gametophytes that produced sporophytes subsequently. The spores were monolete, reniform (mean length 36.49 ± 3.6 × breadth 25.67 ± 4.2 μm) to spheroidal with fimbriate and broad‐winged perispore. The maximum germination was in the Parker's and Thompson's (P&TV) medium with 0.1 mg L−1 thiamin, 0.1 mg L−1 riboflavin, 0.1 mg L−1 biotin, 0.1 mg L−1 pyridoxin, 0.5 mg L−1 nicotinic acid, 100 mg L−1 myoinositol, 0.01 mg L−1 folic acid, and 3 mg L−1 glycine. Spore germination was Vittaria type and gametophyte development was Aspidium type. The cordate gametophyte developed archegonia on the 38th day and antheridia on the 42nd day of spore sowing. Both isolate and composite cultures developed sporophytes indicating the presence of intra‐gametophytic selfing. Based on the maximum number of regenerated gametophytes, two combinations, that is, (i) 2 mg L−1 kinetin, and 0.01 mg L−1 2,4‐dichlorophenoxyacetic acid, and (ii) 0.1 mg L−1 naphthaleneacetic acid and 2 mg L−1 kinetin in P&TV media were recommended for mass propagation of C. kendujharensis.

show abstract