Spore Germination and Prothallium Development Conditions of Lygodium japonicum (Thunb.) Sw.

Abstract

-This study was conducted to determine the optimal conditions of growth medium, temperature, and light quality for efficient propagation of Lygodium japonicum spores. The rate of spore germination and prothalium development was high in Knop and 1/8MS and 1/4MS media, which had low mineral content; in particular, the germination rate exceeded 74%, and the germinated spores developed into heart-shaped prothallia. However, in Knop's medium with the lowest mineral content, a rapid prothallium senescence was observed; in 1/4MS medium, prothallium development was delayed. Germination rate increased with the increase in temperature and reached its maximum, 86.7%, at 30°C; however, at this temperature, the prothallia were thinner and abnormal development of rhizoids was observed compared to normally developed prothallia and rhizoids at 25°C. Therefore, the results suggested that the optimal temperature for L. japonicum spore germination was 25°C. The rate of germination was also measured under different light conditions, and the highest rate of 90.6% was observed under LED red light compared to fluorescent (77.2%) or LED blue (5.4%) lights. The germinated spores developed into heart-shaped prothallia under LED red light; however, 15 days after seeding, prothallium development decreased and the became elongated. In contrast, a normal and continuous development of heart-shaped prothallia was observed under fluorescent light.

Conditions of In Vitro Spore Germination and Prothallium Culture for Sporophyte propagation of Polystichum braunii (Spenn.) Fée

“…대다수의 양치식물 포자는 발아를 위한 영양분을 포함하고 있어 물과 소량의 영양분으로 발아와 초기 전엽체 발달이 진 행되는 것으로 알려져 있다 (Miller 1968). 그러나 종에 따라 발아 후 초기 전엽체 형성에 다량의 영양분이 필요하며, 저 농도 배지에서는 전엽체의 노화 진행속도가 빠르다 (Kwon et al 2016). 따라서 양치식물의 종 특성에 따른 포자발아 연구 는 매우 필요한 것으로 사료된다.…”