Spore Germination and Carbon Metabolism in <i>Fusarium solani</i> V. Changes in Anaerobic Metabolism and Related Enzyme Activities during Development

Rado

Journal of General Microbiology

1971

SUMMARYNet synthesis of protein and RNA in germinating macroconidia of Fusarium solani began at the time of appearance of germ tubes, RNA being formed first; net DNA synthesis began much later. Studies on precursor incorporation indicated that the ungerminated spore, at the time of its removal from the parent mycelium, had a low but real capacity to synthesize both RNA and protein. Leucine incorporation rose very rapidly to a maximum at about 40 min. after harvest; even in the time required for conventional washing and filtration, incorporation capacity increased threefold. Ultracentrifuge profiles showed polysome peaks after I 5 min. incubation ; evidence from ribonuclease treatment was consistent with the existence of polysomes in the spore at the time of removal from the mycelium. Neither protein nor RNA synthesis required a complete medium, but DNA was synthesized only in a medium that supported germination.

Section: Organism and Cultivationmentioning

confidence: 99%

Synthesis of Macromolecules and Polyribosome Formation in Early Stages of Spore Germination in Fusarium solani

Rado

Journal of General Microbiology

1971

American Journal of Botany

“…The endogenous respiration of this fungus, as in many others, proceeds at a fairly high level (Cochrane, 1963). It was possible to lower the level of endogenous activity and at the same time increase the response to added substrate by growing the fungus on a dilute medium (Bonner and Machlis, 1957) followed by aeration in buffer for 2 hr.…”

mentioning

confidence: 92%

“…); water to 3 ml; (5) glyceraldehyde-3-phosphate dehydrogenase (Cochrane and Cochrane, 1966); (6) (Kornberg, 1955); (11) isocitric dehydrogenase (Ochoa, 1955a); (12) malic dehydrogenase (Ochoa, 1955b); and (13) "'malic" enzyme (Ochoa, 1955c). All mixtures contained 0.1 ml of extract and were adjusted to a final volume of 3 ml with distilled water.…”

mentioning

confidence: 99%

“…Following this NADH-requiring step, glycerol-phosphate can then be converted directly to glycerol through the action of a specific phosphatase. This side branch of the EMP scheme would appear to be of particular significance in the fungi since the principal reserve food is often lipid (Cochrane, 1963). Further mention of this enzyme system in Ach.lya will be made below.…”

mentioning

confidence: 99%

“…Spectrophotometric analyses also revealed the presence of a NADHdependent lactic dehydrogenase. Although the formation of lactic acid by several groups of phycomycetes is well documented (Cochrane, 1963;Gleason et al, 1966) this fermentative pathway is not known among members of the Saprolegniales. Lactic dehydrogenase was observed in extracts of both female and male strains of A. ambisexualis.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Respiratory Metabolism in Achlya ambisexualis

Warren¹,

Mullins²

1969

Respiratory Metabolism in Achlya Ambisexualis

Warren

Mullins

1969

American J of Botany

Manometric and spectrophotometric techniques were utilized in studying respiratory metabolism in intact mycelia and cell‐free extracts of the heterothallic water mold, Achlya ambisexualis Raper. Enzymes representing the hexose monophosphate pathway, the Embden‐Meyerhof‐Parnas scheme, the tricarboxylic acid (TCA) cycle and a terminal cytochrome system were demonstrated. In addition, glycerol phosphate dehydrogenase and lactic dehydrogenase activities were observed. Activities of selected enzymes were observed to decline with culture‐age, with the exceptions of glucose‐6‐phosphate dehydrogenase and glycerol‐phosphate dehydrogenase. Oxygen uptake in the male strain more than doubled in the presence of sexual hormone A.