Spore formation and germination inSaccharomyces

Miller, J. J.; Hoffmann-Ostenhof, C

doi:10.1002/jobm.3630040404

Cited by 31 publications

(3 citation statements)

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“…Reviews on yeast ascospore germination (1,5,6) have indicated this to be a relatively unexplored area. More recently, Rousseau et al (7) have studied some physiological and morphological developments which occur during germination such as the change in dry weight, light absorbance, and protein and carbohydrate concentration.…”

Section: Introductionmentioning

confidence: 98%

Germination studies on pure yeast ascospores

Savarese¹

1974

Can. J. Microbiol.

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Pure ascospore suspensions of three strains of Saccharotnyces cereuisiae have been prepared by sequential treatment with 2-mercaptoethanol, Glusulase, and sonication. Light microscopy and acid-fast staining revealed the presence of only ascospores. Various germination media tested using the ascospore preparation revealed different nutritional requirements for the germination of auxotrophic and prototrophic yeast strains. Acid-fast staining of the germinating ascospores was shown to be an effective method for studying the kinetics of ascospore germination. The anaerobic nature of ascospore germination was demonstrated using this staining technique. SAVARESE, J. J. 1974. Germination studies on pure yeast ascospores. Can. J. Microbiol. 20:1517-1522. Nous avons prepart des suspensions pures d'ascospores de trois lignees de Saccharotnyces cereuisiae par traitement sequentiels avec le 2-mercaptoethanol, le glusulase et la sonication. La microscopie photonique et la coloration "acid fast" revelent la presence d'ascospores seulenlent. Des milieux varies pour la germination en utilisant la preparation des ascospores revelent des exigences nutritionnelles pour la germination des lignees auxotrophique et prototrophique. La coloration "acid fast" des ascospores en germination est une mCthode efficace pour etudier les cinetiques de la germination des ascospores. La nature anaerobique des ascospores en germination fut demontree en i~tilisant cette technique de coloration.[Traduit par le journal]

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Section: Introductionmentioning

confidence: 98%

Germination studies on pure yeast ascospores

Savarese¹

1974

Can. J. Microbiol.

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“…Diploid cells homozygous (a/a or a/a) for mating type are not capable of these processes (12,30,34). A state of cellular metabolism conducive to sporulation can be brought about by nitrogen starvation with good aeration and a carbon source other than glucose or ethanol (11,23).…”

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confidence: 99%

Macromolecule Synthesis and Breakdown in Relation to Sporulation and Meiosis in Yeast

et al. 1974

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The time course of synthesis and breakdown of various macromolecules has been compared for sporulating ( a /α) and nonsporulating ( a/a and α/α) yeast cells transferred to potassium acetate sporulation medium. Both types of cells incorporate label into ribonucleic acid and protein. The gel electrophoresis patterns of proteins synthesized in sporulation medium are identical for sporulating and nonsporulating diploids; both are different from electropherograms of vegetative cells. Sporulating and nonsporulating strains differ with respect to deoxyribonucleic acid synthesis; no deoxyribonucleic acid is synthesized in the latter case, whereas the deoxyribonucleic acid complement is doubled in the former. Glycogen breakdown occurs only in sporulating strains. Breakdown of preexisting vegetative ribonucleic acid and protein molecules occurs much more extensively in sporulating than in nonsporulating cells. A timetable of these data is presented.

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“…It is well-established that in Saccharornyces growth can occur both aerobically and anaerobically, and that sporulation is aerobic (Miller and Hoffmann-Ostenhof 1964). The present work indicates that ascospore germination in Saccharomyces occurs under anaerobic conditions (although it is not prevented by the presence of oxygen).…”

Section: Discussionmentioning

confidence: 61%

Observations on acid-fastness and respiration of germinating yeast ascospores

Seigel¹,

Miller²

1971

Can. J. Microbiol.

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1971. Observations on acid-fastness and respiration of germinating yeast ascospores. Can. J. Microbiol. 17: 837-845. Germination of the ascospores of a strain of Saccharomyces cerevisiae Hansen was stimulated by fermentable carbon sources. Nitrogen sources alone had little or no stimulating effect on germination unless glucose was present, but peptone and casein hydrolysate were exceptions. Loss of acid-fastness proved to be a useful criterion of spore germination. Evidence was obtained that germination occurs anaerobically, but is not restricted by oxygen. It is concluded that the respiratory characteristics of the three main phases of the yeast life cycle differ in that growth can occur aerobically or anaerobically, sporulation is restricted to aerobic conditions, and spore germination is anaerobic.

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Spore formation and germination inSaccharomyces

Cited by 31 publications

References 50 publications

Germination studies on pure yeast ascospores

Germination studies on pure yeast ascospores

Macromolecule Synthesis and Breakdown in Relation to Sporulation and Meiosis in Yeast

Observations on acid-fastness and respiration of germinating yeast ascospores

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