Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa

Hossain, Amjad; Osuamkpe, C.; Hossain, Shaikat; Phelps, John Y.

doi:10.1007/s10815-009-9375-x

Cited by 31 publications

(21 citation statements)

References 17 publications

(24 reference statements)

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“…But it must be noted that the method is not suitable for spermatozoa that have been processed, specifically cryopreserved then thawed or column‐washed samples, as these spontaneously develop tail swellings (Hossain et al ., ).…”

Section: Hypo‐osmotic Swelling Testmentioning

confidence: 97%

How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologist's view

Nordhoff

2014

Andrology

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SUMMARYA viable spermatozoon is the prerequisite for initiating fertilization in intracytoplasmic sperm injection. Usually motility is the primary sign used to determine a sperm's viability. However, in every in vitro fertilization (IVF) laboratory cases are observed in which none or only few spermatozoa are motile. This can occur in treatment cycles involving ejaculated samples but is most common in cases where surgically extracted testicular spermatozoa are used. To aid in selection, several techniques have been developed to identify viable spermatozoa from the immotile fraction. Amongst the more commonly used approaches are (i) the hypo-osmotic swelling test (ii) chemical substances for induction of tail movements (iii) the sperm tail flexibility test and (iv) laser-assisted immotile sperm selection. All can be used routinely in an IVF laboratory with each having both strengths and weaknesses. It is the purpose of this short review to focus on the technical issues involved in the performance of each of these techniques and to highlight the advantages and disadvantages of each approach.

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Section: Hypo‐osmotic Swelling Testmentioning

confidence: 97%

How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologist's view

Nordhoff

2014

Andrology

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“…However, false positive and false negative results reduce the test accuracy [53]. According to Hossain et al, the HOS method can not be considered a fully reliable alternative for vital staining because of spontaneously developed tail curling in fresh (5.9%) as well as in frozen (6.7%) sperm samples [54]. …”

Section: What Progress Did We Make?mentioning

confidence: 99%

Processing and selection of surgically-retrieved sperm for ICSI: a review

Verheyen¹,

Popović-Todorović²,

Tournaye³

2017

Basic Clin. Androl.

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Although the technique of intracytoplasmic sperm injection (ICSI) has been a revolution in the alleviation of male infertility, the use of testicular sperm for ICSI was a formerly unseen breakthrough in the treatment of the azoospermic man with primary testicular failure. At the clinical level, different procedures of testicular sperm retrieval (conventional TESE, micro-TESE, FNA/TESA, MESA, PESA) are being performed, the choice is mainly based on the cause of azoospermia (obstructive versus non-obstructive) and the surgeon’s skills. At the level of the IVF laboratory, mechanical procedures to harvest the sperm from the tissue may be combined with enzymatic treatment in order to increase the sperm recovery rates. A number of techniques have been developed for viable sperm selection in males with only immotile testicular sperm available. However, large, well-designed studies on the benefit and safety of one over the other technique are lacking. Despite all the available methods and combinations of laboratory procedures which have a common goal to maximize sperm recovery from testicular samples, a large proportion of NOA patients fail to father a genetically own child. Advanced technology application may improve recovery rates by detection of the testicular foci with active spermatogenesis and/or identification of the rare individual sperm in the testicular suspensions. On the other hand, in vitro spermatogenesis or sperm production from embryonic stem cells or induced pluripotent stem cells might be future options. The present review summarizes the available strategies which aim to maximize sperm recovery from surgically retrieved samples.

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“…As an important source of DNA toxins, smoking was associated with DNA strands breaks formation in spermatozoa (Niu et al ., ). Hypo‐osmotic swelling test (HOS test) as a measure for investigating sperm membrane integrity was extended to include sperm viability assessment (Hossain et al ., ). Also, seminal zinc (Zn) provides a protective role against heavy metal accumulation in semen that demonstrated significant decrease correlated with the extent of smoking (Liu et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Smoking influence on sperm vitality, DNA fragmentation, reactive oxygen species and zinc in oligoasthenoteratozoospermic men with varicocele

et al. 2013

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This study aimed to assess the influence of smoking duration and intensity on sperm vitality, sperm DNA fragmentation, reactive oxygen species (ROS) and zinc (Zn) levels in oligoasthenoteratozoospermic (OAT) men with varicocele (Vx). A total of 246 men were investigated who were divided into OAT nonsmokers, OAT smokers, OAT nonsmokers and OAT smokers with Vx. They were subjected to history taking, clinical examination and semen analysis. In their semen, sperm hypo-osmotic swelling (HOS) test, sperm DNA fragmentation test, seminal ROS and seminal Zn were assessed. The results demonstrated significantly decreased HOS test, seminal Zn level and significantly increased sperm DNA fragmentation, seminal ROS levels in OAT smokers with Vx more than OAT smokers compared with OAT nonsmokers. Smoking intensity, smoking duration and Vx grade demonstrated significant negative correlations with sperm motility, HOS test percentage and significant positive correlations with sperm DNA fragmentation, seminal ROS level. It is concluded that smoking has a negative impact on sperm progressive motility, HOS test, seminal Zn and positive impact on sperm DNA fragmentation, semen ROS level that are exaggerated if Vx is associated being correlated with smoking intensity, smoking duration and Vx grade.

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Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa

Cited by 31 publications

References 17 publications

How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologist's view

How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologist's view

Processing and selection of surgically-retrieved sperm for ICSI: a review

Smoking influence on sperm vitality, DNA fragmentation, reactive oxygen species and zinc in oligoasthenoteratozoospermic men with varicocele

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