Spontaneous Slow Wave and Contractile Activity of the Guinea Pig Prostate

Exintaris, Betty; Klemm, Megan F; Lang, Richard J

doi:10.1097/00005392-200207000-00098

Cited by 36 publications

(88 citation statements)

References 26 publications

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“…Cajal [1] saw these cells as interstitial neurons. Güldner et al [40] demonstrated a system of interconnected ''fibroblasts'' in the stroma of duodenal villi containing thick and thin contractile filaments. The organization (Fig.…”

Section: The Myofibroblasts (Or Myoid Cells) Of the Intestinal Villimentioning

confidence: 99%

“…Further ultrastructural studies, and further investigations into the relationship of these cells to other cells in the pancreas, in particular the intrinsic nerves and smooth muscle cells, are eagerly awaited. Richard Lang and co-workers reported on c-kit positive interstitial cells in the guinea pig prostate that formed a network of interconnecting cells with associations with stromal smooth muscle cells and varicose axon bundles [40]. Further experimentation is underway to ascertain a possible origin of spontaneous slow waves in the guinea pig prostate interstitial cells [40].…”

Section: Interstitial Cells In Glandsmentioning

confidence: 99%

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About the presence of interstitial cells of Cajal outside the musculature of the gastrointestinal tract

Huizinga¹,

Faussone–Pellegrini²

2005

J Cellular Mol Med

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Santiago Ramon y Cajal observed a special cell type that appeared to function as endstructures of the intrinsic nervous system in several organs. These cells were structurally and functionally further characterized in the gut musculature and named interstitial cells of Cajal (ICC). In recent years, interstitial cells have been identified in the vasculature, urinary tract, glands and other organs. Their morphologies and functions are just beginning to be clarified. It is likely that amongst them, subtypes will be discovered that warrant the classification of interstitial cells of Cajal. This “point of view” continues the discussion on the criteria that should be used to identify ICC outside the musculature of the gut.

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Section: The Myofibroblasts (Or Myoid Cells) Of the Intestinal Villimentioning

confidence: 99%

Section: Interstitial Cells In Glandsmentioning

confidence: 99%

About the presence of interstitial cells of Cajal outside the musculature of the gastrointestinal tract

Huizinga¹,

Faussone–Pellegrini²

2005

J Cellular Mol Med

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“…In a series of recent papers, ICC-like cells have been claimed to occur in other organs: vas deferens [25], urethra [26][27][28], bladder [29][30][31][32][33], ureter [34,35], uterus [36,37], blood vessels [38][39][40], lymphatics [41], prostate [42,43], penis [44], mammary gland [45] and pancreas [46].…”

Section: Introductionmentioning

confidence: 99%

Novel type of interstitial cell (Cajal-like) in human fallopian tube

et al. 2005

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We describe here ‐ presumably for the first time‐a Cajal‐like type of tubal interstitial cells (t‐ICC), resembling the archetypal enteric ICC. t‐ICC were demonstrated in situ and in vitro on fresh preparations (tissue cryosections and primary cell cultures) using methylene‐blue, crystal‐violet, Janus‐Green B or Mito Tracker‐Green FM Probe vital stainings. Also, t‐ICC were identified in fixed specimens by light microscopy (methylene‐blue, Giemsa, trichrome stainings, Gomori silver‐impregnation) or transmission electron microscopy (TEM). The positive diagnosis of t‐ICC was strengthened by immunohistochemistry (IHC; CD117/c‐kit+ and other 14 antigens) and immunofluorescence (IF; CD117/c‐kit+ and other 7 antigens). The spatial density of t‐ICC (ampullar‐segment cryosections) was 100–150 cells/mm2. Non‐conventional light microscopy (NCLM) of Epon semithin‐sections revealed a network‐like distribution of t‐ICC in lammina propria and smooth muscle meshwork. t‐ICC appeared located beneath of epithelium, in a 10–15μ thick ‘belt’, where 18±2% of cells were t‐ICC. In the whole lamina propria, t‐ICC were about 9%, and in muscularis ∼7%. In toto, t‐ICC represent ∼8% of subepithelial cells, as counted by NCLM. In vitro, t‐ICC were 9.9±0.9% of total cell population. TEM showed that the diagnostic ‘gold standard’ (Huizinga et al., 1997) is fulfilled by ‘our’ t‐ICC. However, we suggest a ‘platinum standard’, adding a new defining criterion ‐ characteristic cytoplasmic processes (number: 1–5; length: tens of μm; thickness: ±0.5μ; aspect: moniliform; braching: dichotomous; organization: network, labyrinthic‐system). Quantitatively, the ultrastructural architecture of t‐ICC is: nucleus, 23.6±3.2% of cell volume, with heterochromatin 49.1±3.8%; mitochondria, 4.8±1.7%; rough and smooth endoplasmic‐reticulum (1.1±0.6%, 1.0±0.2%, respectively); caveolae, 3.4±0.5%. We found more caveolae on the surface of cell processes versus cell body, as confirmed by IF for caveolins. Occasionally, the so‐called ‘Ca2+‐release units’ (subplasmalemmal close associations of caveolae+endoplasmic reticulum±mitochondria) were detected in the dilations of cell processes. Electrophysiological single unit recordings of t‐ICC in primary cultures indicated sustained spontaneous electrical activity (amplitude of field potentials: 57.26±6.56mV). Besides the CD117/c‐kit marker, t‐ICC expressed variously CD34, caveolins 1&2, α‐SMA, S‐100, vimentin, nestin, desmin, NK‐1. t‐ICC were negative for: CD68, CD1a, CD62P, NSE, GFAP, chromogranin‐A, PGP9.5, but IHC showed the possible existence of (neuro)endocrine cells in tubal interstitium. We call them ‘JF cells’. In conclusion, the identification of t‐ICC might open the door for understanding some tubal functions, e.g. pace‐making/peristaltism, secretion (auto‐, juxta‐ and/or paracrine), regulation of neurotransmission (nitrergic/purinergic) and intercellular signaling, via the very long processes. Furthermore, t‐ICC might even be uncommitted bipotential progenitor cells.

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“…The role of ICCs in the human urinary tract has not been finally defined, but they probably act similarly to the widely known cells of Cajal of the gastrointestinal tract, which is suggested by studies carried out on animal models [21,40,41].…”

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confidence: 99%

Nature of Interstitial Cells of Cajal of the Upper Urinary Tract

Kolęda¹,

Pilecki²

2014

Adv Clin Exp Med

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Interstitial cells of Cajal (ICCs) were discovered in the gastrointestinal tract over 100 years ago and since then numerous digestive tract pathologies involving ICCs have been described. Many researchers explored ICCs presence and function in the upper urinary tract. Currently, we know that ICCs have potential plasticity, their own spontaneous activity and that they are responsible for Ca 2+ waves generation and neuromuscular transmission. ICCs are also involved in the conjugation, propagation and modulation of peristaltic waves in the upper urinary tract. Despite everything we know about ICCs, their role in the pathogenesis of the upper urinary tract abnormalities remains still unclear and results of published studies are confusing. The authors' intention was to review the scientific literature regarding ICCs and to summarise the current knowledge about their nature in the upper urinary tract (Adv Clin Exp Med 2014, 23, 4, 627-632).

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Spontaneous Slow Wave and Contractile Activity of the Guinea Pig Prostate

Cited by 36 publications

References 26 publications

About the presence of interstitial cells of Cajal outside the musculature of the gastrointestinal tract

About the presence of interstitial cells of Cajal outside the musculature of the gastrointestinal tract

Novel type of interstitial cell (Cajal-like) in human fallopian tube

Nature of Interstitial Cells of Cajal of the Upper Urinary Tract

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