Spontaneous self-assembly and disassembly of colloidal gold nanoparticles induced by tetrakis(hydroxymethyl) phosphonium chloride

Gulka, Christopher P.; Wong, Alexis; Wright, David W.

doi:10.1039/c5cc08211k

Cited by 18 publications

(20 citation statements)

References 32 publications

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“…The average particle size was found to be about 102.7 nm with a good zeta potential of −10.1 mV for the WDL-AuNPs (Figure 2c,d). This confirms the stability of the colloids [25]. This stipulates that the obtained AuNPs had a good a repulsive electrostatic force, which provides the mono dispersity of the particles.…”

Section: Resultssupporting

confidence: 77%

Antidiabetic Activity of Gold Nanoparticles Synthesized Using Wedelolactone in RIN-5F Cell Line

et al. 2019

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We synthesized the gold nanoparticles (AuNPs) using wedelolactone (WDL) and characterized them using UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopic (SEM), transmission electron microscopic (TEM), energy dispersive X-ray diffraction, and atomic force microscopic (AFM) studies. The electronic spectrum exhibited an absorption peak at 535 nm. The FT-IR results proved that WDL was stabilized on the surface of AuNPs by acting as a capping or reducing agent. The crystalline structure was affirmed by XRD pattern and the spherical shape of WDL-AuNPs was evidenced by SEM, TEM, and AFM. The synthesized WDL-AuNPS were evaluated for anti-diabetic activity in pancreatic RIN-5F cell lines. In vitro results showed that WDL-AuNPs did not only improve the insulin secretion affected by di-(2-ethylhexyl) phthalate (DEHP), but also the cell viability in RIN5F cells. WDL-AuNPs treatment modulates the pro-apoptotic proteins and anti-apoptotic proteins expression to prevent the cells undergoing apoptosis in DEHP-exposed RIN-5F cells. The exposure of DEHP causes an increase in ROS production and lipid peroxidation levels. The free radical scavenging and antioxidant properties of WDL-AuNPs increase the deleterious effect caused by DEHP. On the other side, WDL-AuNPs increase mRNA expressions of insulin-signaling proteins in RIN-5F cells. This study concludes that WDL-AuNPs can be successfully used to regulate the expression of Bcl-2 family proteins, reduce lipid peroxidation, and to improve the secretion of antioxidants and insulin through the GLUT2 pathway in RIN-5F cell lines.

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Section: Resultssupporting

confidence: 77%

Antidiabetic Activity of Gold Nanoparticles Synthesized Using Wedelolactone in RIN-5F Cell Line

et al. 2019

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“…By dissolving PLGA polymers in acetone to create an oil phase and gently pipetting the oil phase dropwise to the water phase under sonication, PLGA-THPC nanoparticles were formed. When the nanoparticles were placed in a Petri dish and irradiated with a UV light, THPC served as a reducing agent for Ag + in solution, as shown mechanistically in Figure 1 b. THPC is a strong reducing agent for synthesizing nanoparticles [ 32 , 37 ]. Photoreduction, along with adding a strong reducing agent, by reducing silver nitrate, allowed for a change in its morphology to a star shape.…”

Section: Resultsmentioning

confidence: 99%

“…Herein, a combinatorial method is described where a phosphonium salt—tetrakis (hydroxymethyl) phosphonium chloride (THPC)—is adsorbed on PLGA in the presence of silver nitrate seed. THPC has been used as a crosslinker of hydrogels [ 31 ] and a reducing agent for metal nanoparticles [ 32 , 33 ]. It is hypothesized that the chemical reduction of silver in the presence of THPC impregnated in PLGA nanoparticles causes the intraparticle morphology to vary.…”

Section: Introductionmentioning

confidence: 99%

Heteromer Nanostars by Spontaneous Self-Assembly

et al. 2017

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Heteromer star-shaped nanoparticles have the potential to carry out therapeutic agents, improve intracellular uptake, and safely release drugs after prolonged periods of residence at the diseased site. A one-step seed mediation process was employed using polylactide-co-glycolic acid (PLGA), polyvinyl alcohol (PVA), silver nitrate, and tetrakis(hydroxymethyl)phosphonium chloride (THPC). Mixing these reagents followed by UV irradiation successfully produced heteromer nanostars containing a number of arm chains attached to a single core with a high yield. The release of THPC from heteromer nanostars was tested for its potential use for breast cancer treatment. The nanostars present a unique geometrical design exhibiting a significant intracellular uptake by breast cancer cells but low cytotoxicity that potentiates its efficacy as drug carriers.

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“…AuNPs were synthesized according to the previous report with several modifications with reactants concentrations. , THPC from an 80% aqueous solution (0.12 mL) was diluted in DI water (10 mL). Sodium hydroxide solution (0.2 M, 1.5 mL) and the diluted THPC solution (1 mL) were then added to DI water (45 mL).…”

Section: Methodsmentioning

confidence: 99%

Mesoporous Silica Nanoparticles Doped with Gold Nanoparticles for Combined Cancer Immunotherapy and Photothermal Therapy

Ong

Geun

Kim

2019

ACS Appl. Bio Mater.

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Cancer immunotherapy is a treatment that utilizes the host immune system to fight against cancer. Inducing a cancer antigenspecific adaptive immune response is key in cancer immunotherapy. Although diverse immune cells including dendritic cells (DCs) and T cells infiltrate a tumor, the activation of such immune cells is inhibited, owing to an immunosuppressive tumor microenvironment. In this study, we propose mesoporous silica nanoparticles (XL-MSNs) decorated with gold nanoparticles (Au@XL-MSNs) for the delivery of a high amount of CpG-ODNs to the tumor site to activate DCs infiltrated within the tumor for the induction of an antigen-specific adaptive immune response. During an in vitro test, CpG-ODNs delivered using Au@XL-MSNs were shown to be more effectively internalized by bone-marrow-derived dendritic cells (BMDCs), resulting in an enhanced expression of costimulatory molecules and an increased secretion of pro-inflammatory cytokines compared to soluble CpG-ODNs. Furthermore, an in vivo test demonstrated a more significant tumor growth inhibition and an enhanced survival rate result from the intratumoral injection of Au@XL-MSN-CpG compared to that treated using soluble CpG-ODNs. Furthermore, through the induction of a photothermal effect based on the assembled AuNPs on XL-MSNs, an enhancement of the cancer immunotherapy was achieved by generating a cancer antigen at the tumor site, which can be processed by tumor-infiltrated DCs. These findings suggest that our approach can be applied as a synergistic platform for efficient cancer immunotherapy, enabling a delivery of immunostimulating signals as well as in situ antigen generation through a photothermal effect.

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Spontaneous self-assembly and disassembly of colloidal gold nanoparticles induced by tetrakis(hydroxymethyl) phosphonium chloride

Cited by 18 publications

References 32 publications

Antidiabetic Activity of Gold Nanoparticles Synthesized Using Wedelolactone in RIN-5F Cell Line

Antidiabetic Activity of Gold Nanoparticles Synthesized Using Wedelolactone in RIN-5F Cell Line

Heteromer Nanostars by Spontaneous Self-Assembly

Mesoporous Silica Nanoparticles Doped with Gold Nanoparticles for Combined Cancer Immunotherapy and Photothermal Therapy

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