Spontaneous release of DNA in sequential genetic order by Bacillus subtilis

Borenstein, Shoshana; Ephrati-Elizur, Erela

doi:10.1016/0022-2836(69)90216-2

Cited by 97 publications

(60 citation statements)

References 17 publications

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“…Early experiments revealed that this DNA could transform competent strains (Catlin, 1960;Takahashi, 1962) and suggested that cell death could not always account for the levels of extracellular DNA observed (Smithies & Gibbons, 1955;Ottolenghi & Hotchkiss, 1962). Outgrowing spores of B. subtilis 168 were then found to release genetic markers in map order, implying the existence of a specific mechanism of secretion in B. subtilis (Borenstein & Ephrati-Elizur, 1969).…”

Section: Introductionmentioning

confidence: 99%

DNA secretion and gene-level selection in bacteria

Draghi

Turner

2006

Microbiology

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Natural genetic transformation can facilitate gene transfer in many genera of bacteria and requires the presence of extracellular DNA. Although cell lysis can contribute to this extracellular DNA pool, several studies have suggested that the secretion of DNA from living bacteria may also provide genetic material for transformation. This paper reviews the evidence for specific secretion of DNA from intact bacteria into the extracellular environment and examines this behaviour from a population-genetics perspective. A mathematical model demonstrates that the joint action of DNA secretion and transformation creates a novel type of gene-level natural selection. This model demonstrates that gene-level selection could explain the existence of DNA secretion mechanisms that provide no benefit to individual cells or populations of bacteria. Additionally, the model predicts that any trait affecting DNA secretion will experience selection at the gene level in a transforming population. This analysis confirms that the secretion of DNA from intact bacterial cells is fully explicable with evolutionary theory, and reveals a novel mechanism for bacterial evolution.

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Section: Introductionmentioning

confidence: 99%

DNA secretion and gene-level selection in bacteria

Draghi

Turner

2006

Microbiology

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“…When spores are germinated DNA replication begins in a sequential and synchronous manner. (Borenstein and Ephrati-Elizur, 1969). These characteristics have been used to refine the original mapping techniques that were elegantly developed by Sueoka and Yoshikawa (1963) and Yoshikawa and Sueoka (1963a,b).…”

Section: Localization Df Mutationsmentioning

confidence: 93%

“…Lorence and Nester, 1967;Nasser and Nester, 1967 asa. Harford and Sueoka, 1970 See Dubnau, 1970 Dul andFreese et at., 1964Reeve et at., 1973Bazill and Karamata, 1972Borenstein and Ephrati-Elizur, 1969;Karamata andGross, 1970 Karamata andGross, 1970;Gross, 1967 Bazill andRe tie f, 1969;Borenstein and EphratiElizur, 1969;Karamata and Gross, 1970 Korch and Doi, 1971Champney and Jensen, 1969Goldthwaite et al, 1970Takahashi, 1965 Grant andSimon, 1969 Unable to grow on fructose; gene order by transformation is Gay M. S. Pritchard, 1963, 1965; M. S. Kelly, 1967a,b;T. J. Kelly and Smith, 1970 UDP:glucose pyrophosphorylase but does not have signifi- et al, Goldthwait...…”

Section: Localization Df Mutationsmentioning

confidence: 97%

Bacillus subtilis

Young¹,

Wilson²

1974

Bacteria, Bacteriophages, and Fungi

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“…Although apoptosis and necrosis are considered to be the main processes for producing cirDNA, numerous studies have demonstrated that cirDNA can also be derived from active cellular release mechanisms [7][8][9][10][11][12]. Further examination showed that actively released DNA is often associated with RNA and a lipid-protein complex, can translocate to neighboring or remote parts of the body, enter target cells, and alter their biology [12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

A historical and evolutionary perspective on the biological significance of circulating DNA and extracellular vesicles

Aucamp

Bronkhorst

Badenhorst

et al. 2016

Cell. Mol. Life Sci.

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The discovery of quantitative and qualitative differences of the circulating DNA (cirDNA) between healthy and diseased individuals inclined researchers to investigate these molecules as potential biomarkers for non-invasive diagnosis and prognosis of various pathologies. However, except for some prenatal tests, cirDNA analyses have not been readily translated to clinical practice due to a lack of knowledge regarding its composition, function, and biological and evolutionary origins. We believe that, to fully grasp the nature of cirDNA and the extracellular vesicles (EVs) and protein complexes with which it is associated, it is necessary to probe the early and badly neglected work that contributed to the discovery and development of these concepts. Accordingly, this review consists of a schematic summary of the major events that developed and integrated the concepts of heredity, genetic information, cirDNA, EVs, and protein complexes. Cir-DNA enters target cells and provokes a myriad of gene regulatory effects associated with the messaging functions of various natures, disease progression, somatic genome variation, and transgenerational inheritance. This challenges the traditional views on each of the former topics. All of these discoveries can be traced directly back to the iconic works of Darwin, Lamarck, and their followers. The history of cirDNA that has been revisited here is rich in information that should be considered in clinical practice, when designing new experiments, and should be very useful for generating an empirically up-to-date view of cirDNA and EVs. Furthermore, we hope that it will invite many flights of speculation and stimulate further inquiry into its biological and evolutionary origins.

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Spontaneous release of DNA in sequential genetic order by Bacillus subtilis

Cited by 97 publications

References 17 publications

DNA secretion and gene-level selection in bacteria

DNA secretion and gene-level selection in bacteria

Bacillus subtilis

A historical and evolutionary perspective on the biological significance of circulating DNA and extracellular vesicles

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