Phage tail-like particles, known as tailocins, are sophisticated nanomolecular machines with bactericidal activity. They are produced by various bacteria to gain fitness advantages in the environment, and hence they play a critical role in bacterial ecology across all known habitats. Here we characterized phage tail-like particles produced by Dickeya dadantii strain 3937 - one of the widest-characterized members of plant pathogenic Soft Rot Pectobacteriaceae (SRP). Tailocins were induced from D. dadantii strain 3937 cells with mitomycin C and visualized and characterized with microscopic techniques (TEM and AFM). They were ca. 166 nm long, tubes surrounded by contractive sheaths with baseplates with tail fibers at one end. Furthermore, we identified a 22-kb cluster in the genome of D. dadantii 3937 involved in their synthesis that expressed high homology to the cluster coding for the tail of the Enterobacteriophage P2. The D. dadantii 3937 tailocins were named dickeyocins P2D1 (=phage P2-like dickeyocin 1). P2D1 were prone to inactivation by pH (3.5-12), temperature (4-50 C), and elevated osmolarity (NaCl concentration: 0.01-1 M). P2D1 could kill different Dickeya spp.; however, they were unable to kill any strain of Pectobacterium spp. tested. Likewise, they were not toxic to Caenorhabditis elegans.