Spontaneous Malignant Transformation of Human Mesenchymal Stem Cells Reflects Cross-Contamination: Putting the Research Field on Track – Letter

Torsvik, Anja; Røsland, Gro Vatne; Svendsen, Agnete; Molven, Anders; Immervoll, Heike; McCormack, Emmet; Lønning, Per Eystein; Primon, Monika; Sobala, Ewa; Tonn, Joerg‐Christian; Goldbrunner, Roland; Schichor, Christian; Mysliwietz, Josef; Lah, Tamara T.; Motaln, Helena; Knappskog, Stian; Bjerkvig, Rolf

doi:10.1158/0008-5472.can-10-1305

Cited by 266 publications

(174 citation statements)

References 15 publications

(16 reference statements)

Supporting

Mentioning

169

Contrasting

Unclassified

Order By: Relevance

“…cross-contamination with tumor cell lines. [33][34][35] Acknowledging the error, we followed stringent working procedures and verified that the cells in our studies were not contaminated during the long-term in vitro culture. In this study, all of the hUC-MSC clones underwent a decrease in telomerase activity and shortening of telomere length and senesced in culture ultimately.…”

Section: Discussionmentioning

confidence: 99%

Long-term cultured mesenchymal stem cells frequently develop genomic mutations but do not undergo malignant transformation

et al. 2014

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Long-term cultured mesenchymal stem cells frequently develop genomic mutations but do not undergo malignant transformation

et al. 2014

View full text Add to dashboard Cite

“…Regarding the challenges of MSCs therapies in general, MSCs spontaneous transformation in culture leading to tumorigenesis is a possible occurrence with invitro murine MSCs cultures, and less frequently with human MSCs [197][198][199][200][201][202][203][204][205][206]. Two hours after irradiation, most of the aMSCs DNA damage/repair-related genes showed up regulation that disappeared within 6 hours after IR.…”

Section: Chapter 7 Discussionmentioning

confidence: 99%

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

et al. 2016

View full text Add to dashboard Cite

“…Recently, the use of cell lines has been questioned again as reports show high incidence of cross-contaminations between cell lines with all disastrous consequences, 41 for which the ATCC has developed a cell line identification standard. 42 One advantage of the cell lines series used here is that they all were previously characterized at genetic level, 18 thereby allowing for checking cell identities and excluding cross-contaminations.…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease

Mohseny

Machado

Cai

et al. 2011

Laboratory Investigation

157

151

View full text Add to dashboard Cite

Cancer cell lines represent in vitro models for studying malignancies, general cell biology, drug discovery and more. Whether they can be considered as exact representative models of the parental tumors remains uncertain given the acquisition of additional ex vivo changes of the cells and the lack of tissue architecture and stroma. Previously, within the EuroBoNeT consortium, we characterized a collection of bone sarcoma cell lines on genomic and proteomic level. Here, we address the phenotypical and functional characterization of the unique set of osteosarcoma cell lines (n ¼ 19) in vitro and in vivo. For functional analysis of differentiation capacity, cells were stimulated towards osteoblasts, adipocytes and chondrocytes. Furthermore, all cell lines were injected subcutaneously and intramuscularly into nude mice to assay their in vivo tumor formation capacity as well as for phenotypical analysis of the tumors. All formed tumors were further characterized histologically and immunohistochemically. Out of 19 cell lines, 17 (89%) showed adipogenic differentiation, 13/19 (68%) could differentiate towards osteoblasts and in 6/19 (32%) cell lines chondrogenic differentiation was evident. About half of the cell lines (8/19, 42%) produced tumors in vivo after subcutaneous and intramuscular injections. Several cell lines showed invasion into adjacent tissues and one tumor developed several lung metastases. The use of cell lines, especially in cancer research, is of paramount importance. Here, we identify comprehensively characterized osteosarcoma cell lines, which robustly represent clinical osteosarcoma providing researchers useful in vitro and in vivo models to study the genetics and functional characteristics of this highly malignant neoplasm.

show abstract

Spontaneous Malignant Transformation of Human Mesenchymal Stem Cells Reflects Cross-Contamination: Putting the Research Field on Track – Letter

Cited by 266 publications

References 15 publications

Long-term cultured mesenchymal stem cells frequently develop genomic mutations but do not undergo malignant transformation

Long-term cultured mesenchymal stem cells frequently develop genomic mutations but do not undergo malignant transformation

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease

Contact Info

Product

Resources

About