Spontaneous extraskeletal osteosarcoma with various histological growth patterns in the abdominal wall of an ICR mouse

Ito, Tetsuya; Katoh, Yoshitaka; Shimada, Yuko; Ohnuma-Koyama, Aya; Takahashi, Naofumi; Kuwahara, Maki; Harada, Takanori

doi:10.1293/tox.2015-0046

Cited by 3 publications

(5 citation statements)

References 17 publications

(21 reference statements)

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“…An IHC stain for sp7, also known as Osterix, a transcription factor that induces mesenchymal cells to differentiate into osteoblasts and subsequently into osteocytes, is currently available. IHC evaluations of 2 extraskeletal osteosarcomas, 1 each in a mouse and a rat, found good positive staining of neoplastic cells (Ito et al 2016; Nagaike et al 2011). This suggests sp7 may be a useful marker in the diagnosis of highly cellular osteosarcomas in which osteoid formation is minimal or absent.…”

Section: Discussionmentioning

confidence: 97%

Immunohistochemical Characterization of Sarcomas in Trp53+/− Haploinsufficient Mice

et al. 2017

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The use of immunohistochemical (IHC) staining in determining and/or confirming the cellular origin of poorly differentiated sarcomas was evaluated in this study. Sarcomatous neoplasms were evaluated in a research study conducted in two strains of p53+/− haploinsufficient mice. The most common neoplasms were undifferentiated sarcomas, followed by osteosarcomas, and rhabdomyosarcomas (RMSs). The RMSs were poorly differentiated and appeared similar to the pleomorphic, or adult type, RMS of humans. All sarcomas stained positive by IHC for the mesenchymal cell intermediate filament vimentin. The RMSs were identified by positive IHC staining for myogenin, a transcription factor specific to skeletal muscle. Osteosarcomas were easily identifiable on hematoxylin and eosin (H&E) stained slides; no generally accepted IHC stain specific for bone is presently available. Some of the undifferentiated sarcomas contained numerous macrophages that stained positive for F4/80, a macrophage marker; the positive-staining cells were considered to be infiltrating macrophages. One-third of the neoplasms observed in this study were associated with subcutaneous implanted electronic microchips, used for animal identification. Based upon histopathologic evaluation and IHC staining, it was not possible to distinguish neoplasms associated with subcutaneous microchips from neoplasms not associated with microchips.

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Section: Discussionmentioning

confidence: 97%

Immunohistochemical Characterization of Sarcomas in Trp53+/− Haploinsufficient Mice

et al. 2017

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“…Osteosarcoma is a malignant tumor that normally develops in the bone tissue; however, there have been few reports on extraskeletal osteosarcoma (EO) in rats and mice 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 . Here, we describe the first reported case of spontaneous EO in the neck skeletal muscle of a mouse.…”

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confidence: 99%

“…Osteocalcin and osterix are reliable markers for osteosarcoma, while myogenin is a marker for rhabdomyosarcoma. Some EO cases have been shown to be positive for vimentin, S-100, and α-SMA 2 , 3 , 4 , 5 , 6 , 7 . PCNA was used to determine proliferation activity.…”

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confidence: 99%

Spontaneous extraskeletal osteosarcoma in the neck skeletal muscle of a Crlj:CD1 (ICR) mouse

et al. 2021

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Extraskeletal osteosarcoma is extremely rare in humans and animals, especially in rodents. This is the first case report on spontaneous extraskeletal osteosarcoma in the neck skeletal muscle of a Crlj:CD1 (ICR) mouse (36 weeks, dead). Necropsy revealed a solid white mass located in the neck skeletal muscle (scalenus muscle). Histological examination showed that the tumor consisted of atypical polygonal cells, a small osteoid clump, and bone tissue. Mitotic figures were observed. Serial sections showed that neoplastic cells lacked clear invasive proliferation to adjacent normal skeletal muscle and continuity with normal bone tissue. Immunohistochemical analysis showed that the neoplastic cells were positive for osteocalcin, osterix, vimentin, and S-100. Based on these results, the tumor was diagnosed as extraskeletal osteosarcoma in the neck skeletal muscle.

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“…Specifically, it has been reported in the skin (mouse) 1 , subcutaneous tissue (rat) 2 , 3 , thoracic cavity (rat) 4 , abdominal wall (mouse) 5 , stomach (rat) 6 , and cecum (rat) 7 . This paper describes the first case of spontaneous extraskeletal osteosarcoma detected in the duodenum of a mouse.…”

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confidence: 99%

“…Osteocalcin and osteonectin are well-known reliable markers for osteogenic tumors, and some extraskeletal osteosarcomas are said to positively react with anti-vimentin, S-100 protein, and α-SMA antibodies 2 , 3 , 5 , 6 . Judging from the abovementioned results, the present tumor was diagnosed as an extraskeletal osteosarcoma originating from the duodenum.…”

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confidence: 99%

Spontaneous extraskeletal osteosarcoma in the duodenum of a Crlj:CD1 (ICR) mouse

et al. 2016

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Extraskeletal osteosarcoma is a very rare tumor in humans and animals. This paper describes a case of extraskeletal osteosarcoma observed in the duodenum of a male ICR mouse. Grossly, a solid mass pushing up the tunica serosa was observed in the duodenal wall. Histologically, the tumor was located in the lamina propria mucosae and tela mucosa. Neoplastic cells densely proliferated in these areas, and replaced of the normal tissue components. A small amount of osteoid and a small clump of bone tissue were observed in the area of neoplastic cell proliferation, especially in the lamina propria mucosae. Neoplastic cells consisted of atypical polygonal cells and pleomorphic spindle-shaped cells, and the former were predominant. Mitotic figures were occasionally observed. Neither invasion of vessels in the duodenum nor metastasis to distant organs was observed. There were no skeletal tumors in the body. Immunohistochemically, the neoplastic cells were positive for anti-osteocalcin, osteonectin, vimentin, and S-100 protein. Judging from these results, the present tumor was diagnosed as extraskeletal osteosarcoma. This is the first report of spontaneous extraskeletal osteosarcoma arising from the duodenum of a mouse.

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Spontaneous extraskeletal osteosarcoma with various histological growth patterns in the abdominal wall of an ICR mouse

Cited by 3 publications

References 17 publications

Immunohistochemical Characterization of Sarcomas in Trp53+/− Haploinsufficient Mice

Immunohistochemical Characterization of Sarcomas in Trp53+/− Haploinsufficient Mice

Spontaneous extraskeletal osteosarcoma in the neck skeletal muscle of a Crlj:CD1 (ICR) mouse

Spontaneous extraskeletal osteosarcoma in the duodenum of a Crlj:CD1 (ICR) mouse

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