Spontaneous DNA Synthesis in Rheumatoid Arthritis: Evidence of Enhanced Circulating Non‐T‐Cell Proliferation

Al‐Balaghi, S.; Ström, Håkan; Möller, Erna

doi:10.1111/j.1365-3083.1983.tb00820.x

Cited by 15 publications

(8 citation statements)

References 27 publications

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“…A number of explanations may be suggested for this lack of correlation, which contrasts with previous findings by others of a strong correlation between lymphocyte activation and disease activity (6,7). Serum IL-2R may accumulate over time, and thus may be less sensitive to fluctuations in clinical disease activity.…”

Section: Discussioncontrasting

confidence: 59%

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Elevated soluble interleukin‐2 receptor levels in the sera and synovial fluids of patients with rheumatoid arthritis

Keystone

Snow

Bombardier

et al. 1988

Arthritis & Rheumatism

106

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In a previous study, we used an enzyme‐linked immunosorbent assay to measure soluble human interleukin‐2 receptors (IL‐2R), and found that when activated lymphocytes produce cell‐associated IL‐2R, they also release a soluble form of IL‐2R into culture supernatants in vitro. Soluble IL‐2R have also been detected circulating in vivo at low levels in the serum of healthy individuals, and at abnormal levels in a variety of diseases, particularly those where immune dysfunction is thought to play an important role. We therefore evaluated serum IL‐2R levels in 77 patients with rheumatoid arthritis (RA), and compared them with levels in 46 age‐matched healthy controls. Nineteen additional RA patients with concurrently obtained sera and synovial fluid (SF) samples were compared with 14 patients with osteoarthritis of the knee or hip. The serum IL‐2R levels were significantly elevated in RA patients, compared with the control groups (P < 0.0001). Serum IL‐2R levels in the RA patients did not correlate with disease activity as determined by a variety of clinical and laboratory parameters. RA SF IL‐2R levels were significantly higher than corresponding RA serum IL‐2R levels (P = 0.0001). No such difference was noted in the osteoarthritis group, where serum and SF IL‐2R levels were comparable with serum levels in healthy controls. These findings support the hypothesis that in vivo lymphocyte activation plays an important role in RA; moreover, soluble IL‐2R measurement in serum and SF may be a very useful way to identify patients at risk for, or manifesting, a chronic immune‐mediated inflammatory arthropathy.

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Section: Discussioncontrasting

confidence: 59%

“…Serum IL-2R levels were comparable with those in healthy controls (data not shown) and lower than those in the RA patient group (P = 0.002) (Figure 2B). In 6 OA patients, SF were obtained at the time of serum sampling. Soluble IL-2R levels in these samples were generally lower than those observed in RA SF.…”

Section: Isd"mentioning

confidence: 99%

Elevated soluble interleukin‐2 receptor levels in the sera and synovial fluids of patients with rheumatoid arthritis

Keystone

Snow

Bombardier

et al. 1988

Arthritis & Rheumatism

106

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“…Interestingly, malnutrition [13] and severe sepsis [15] have been shown to be associated with an impairment in the monocyte release of interleukin 1 in vitro, which is in line with the present indication of an inverse relationship between SpP and interleukin 1 activity in infected malnourished patients. The finding that SpP is higher after 24 h than after 72 h of cell culture has previously been reported in patients with active rheumatoid arthritis [1], and is suggestive of a lymphocyte population with a high rate of DNA synthesis and a short life span. Consequently, characteristics of spontaneous as well as mitogen-induced lymphocyte proliferation may be overlooked using longer periods of cell culture.…”

Section: Discussionsupporting

confidence: 77%

Spontaneous Lymphocyte Proliferation during Trauma and Infection

Soop¹,

Soop²,

Sundqvist³

1988

Scand J Immunol

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Spontaneous lymphocyte proliferation (SpP), measured in vitro as the rate of [14C]thymidine incorporation in blood lymphocytes, was investigated in non-infected postoperative patients, infected postoperative patients, and healthy volunteers, with 72, 24, and 3 h of lymphocyte culture. With 24-h cultures, infected postoperative patients showed 17-fold higher SpP than non-infected postoperative patients (2527 +/- 1552 versus 151 +/- 77 cpm, mean +/- SD, P less than 0.001) and 37-fold higher SpP than healthy volunteers (P less than 0.001). Postoperative patients without infection had twice as high SpP as healthy volunteers (P less than 0.001). Lymphocytes harvested after 24 h of cell culture showed significantly higher SpP than corresponding values at 72 and 3 h, in patients as well as in healthy volunteers (P less than 0.01). Infected postoperative patients showed a higher SpP than non-infected patients after only 3 h of cell culture (270 +/- 192 versus 48 +/- 10 cpm, P less than 0.001). An inverse correlation was observed between the level of SpP and body temperature in patients with postoperative infection (r = -0.62, P less than 0.05). The results indicate that lymphocytes are activated by uncomplicated surgery and particularly by postoperative infection, and that characteristics of SpP are reproducible in short cell-culture periods, which suggests that in vitro measurements of SpP may be of value in the detection of severe postoperative infection.

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“…Spontaneous DNA synthesis in lymphocytes has been reported to be elevated in patients with connective tissue disorders (27,28), allergy (29), infections (19,30), and malignant tumors (31). IL-6 in serum also exhibits elevation in acute and chronic inflammation and in septic shock and injury (32,33).…”

Section: Discussionmentioning

confidence: 99%